In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder ...In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder and maltose were the optimal nitrogen and carbon sources,and MgSO4 was the optimal inorganic salt.The orthogonal experiment results showed that the optimal combination of liquid seed culture medium for Grifola frondosa was:20%potato,0.75%soybean powder,5%maltose,0.1%KH2PO4,0.05%MgSO4,0.001%VB1.Using the optimized medium,shaking culture for 10 d under the inoculum amount of 5%,the temperature of 28℃,and the rotation speed of 150 r/min,the dry weight of mycelium exceeded 1.3228 g/100mL.展开更多
A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employe...A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode.展开更多
文摘In this paper,the liquid seed medium of Grifola frondosa was optimized by single factor and orthogonal experiments using the shake flask culture method.The single factor experiments results showed that soybean powder and maltose were the optimal nitrogen and carbon sources,and MgSO4 was the optimal inorganic salt.The orthogonal experiment results showed that the optimal combination of liquid seed culture medium for Grifola frondosa was:20%potato,0.75%soybean powder,5%maltose,0.1%KH2PO4,0.05%MgSO4,0.001%VB1.Using the optimized medium,shaking culture for 10 d under the inoculum amount of 5%,the temperature of 28℃,and the rotation speed of 150 r/min,the dry weight of mycelium exceeded 1.3228 g/100mL.
基金supported by the National Natural Science Foundation of China (Nos.30973627 and 30772640)the public projects of the State Oceanic Administration (No.2010418022-3)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University (No.IRT0944)the Natural Science Fund of Shandong Province,P.R.China (No.ZR2009CZ016)
文摘A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode.
