目的研究分化抗原簇蛋白24(cluster of differentiation-24,CD24)在宫颈癌的临床分期和病理组织分型中的表达和关系。方法以宫颈癌40例为观察组1;宫颈上皮内瘤变(CIN)35例为观察组2;慢性宫颈炎正常宫颈组织20例为对照组。三组患者均采...目的研究分化抗原簇蛋白24(cluster of differentiation-24,CD24)在宫颈癌的临床分期和病理组织分型中的表达和关系。方法以宫颈癌40例为观察组1;宫颈上皮内瘤变(CIN)35例为观察组2;慢性宫颈炎正常宫颈组织20例为对照组。三组患者均采用免疫组化链霉菌抗生物素蛋白-过氧化物酶方法(streptavidin peroxidase,SP)进行CD24表达检测。结果宫颈癌及CIN患者中CD24蛋白表达主要在癌细胞及病变细胞的细胞膜和胞浆中。宫颈癌细胞CD24阳性36例,阳性率为90%;CIN患者宫颈组织中CD24阳性15例,阳性率为42.9%;正常宫颈组织中CD24的表达阳性为0例,阳性率为0.00%。阳性率随着宫颈病变程度逐渐升高,三组间差异有统计学意义(P<0.05),宫颈癌的组织病理分型及临床分期、CIN的组织病理分级的CD24表达阳性率差异无统计学意义(P>0.05)。结论在宫颈癌及CIN宫颈组织中CD24表达率明显高于正常宫颈组织,且与病理组织分型及临床分期无关。CD24的高表达可能为宫颈癌的治疗提供新理论依据。展开更多
Foxp3（Forkhead box protein 3）是转录因子家族中的一个成员,是CD4＋CD25＋调节性T细胞（Tregs）特异的标志物[1,2]。在一些肿瘤病人的肿瘤组织和外周血中均存在Foxp3表达增强和Tregs增多的现象[3,4],其通过协助肿瘤细胞逃避免疫系统...Foxp3（Forkhead box protein 3）是转录因子家族中的一个成员,是CD4＋CD25＋调节性T细胞（Tregs）特异的标志物[1,2]。在一些肿瘤病人的肿瘤组织和外周血中均存在Foxp3表达增强和Tregs增多的现象[3,4],其通过协助肿瘤细胞逃避免疫系统的监视,促进肿瘤的免疫逃逸。而Foxp3是Treg发挥作用的关键调节基因。本研究通过对宫颈癌、展开更多
Objective. Co- expression patterns of the genes implicated in angiogenesis and tumor invasion in cervical carcinoma cells were investigated together with invasive activity of tumor cells. Transcript levels of those ge...Objective. Co- expression patterns of the genes implicated in angiogenesis and tumor invasion in cervical carcinoma cells were investigated together with invasive activity of tumor cells. Transcript levels of those genes were also compared between tumor cells and normal cervical tissues. Methods. Real-time quantitative RT- PCR analysis was conducted on selected 11 genes (total VEGF- A, VEGF121,VEGF165,VEGF189,VEGF- B, C and D, bFGF, dThdPase, MMP- 2 and uPA) using 11 cervical carcinoma cell lines and 14 normal cervical tissues. Protein ex-pression of VEGF- C and MMP- 2 and invasive activity of tumor cells were evaluated for each cell line by sandwich ELISA and haptoinvasion assay, respectively. Results. Gene co-expression analysis revealed the significant correlation between angiogenic factors and proteinases in malignant but not in normal cervical samples. Gene or protein expression levels of VEGF- C and MMP- 2 were well correlated with the number of invaded tumor cells. VEGF- A splicing variants were increased in malignant compared to normal cervical samples but not associated with the invasive activity of the cells. Conclusion. VEGF- C and MMP- 2 were closely related to invasive phenotype of tumor cells, whereas VEGF- A isoforms were considered to be involved in cervical carcinogenesis.展开更多
文摘目的研究分化抗原簇蛋白24(cluster of differentiation-24,CD24)在宫颈癌的临床分期和病理组织分型中的表达和关系。方法以宫颈癌40例为观察组1;宫颈上皮内瘤变(CIN)35例为观察组2;慢性宫颈炎正常宫颈组织20例为对照组。三组患者均采用免疫组化链霉菌抗生物素蛋白-过氧化物酶方法(streptavidin peroxidase,SP)进行CD24表达检测。结果宫颈癌及CIN患者中CD24蛋白表达主要在癌细胞及病变细胞的细胞膜和胞浆中。宫颈癌细胞CD24阳性36例,阳性率为90%;CIN患者宫颈组织中CD24阳性15例,阳性率为42.9%;正常宫颈组织中CD24的表达阳性为0例,阳性率为0.00%。阳性率随着宫颈病变程度逐渐升高,三组间差异有统计学意义(P<0.05),宫颈癌的组织病理分型及临床分期、CIN的组织病理分级的CD24表达阳性率差异无统计学意义(P>0.05)。结论在宫颈癌及CIN宫颈组织中CD24表达率明显高于正常宫颈组织,且与病理组织分型及临床分期无关。CD24的高表达可能为宫颈癌的治疗提供新理论依据。
文摘Foxp3（Forkhead box protein 3）是转录因子家族中的一个成员,是CD4＋CD25＋调节性T细胞（Tregs）特异的标志物[1,2]。在一些肿瘤病人的肿瘤组织和外周血中均存在Foxp3表达增强和Tregs增多的现象[3,4],其通过协助肿瘤细胞逃避免疫系统的监视,促进肿瘤的免疫逃逸。而Foxp3是Treg发挥作用的关键调节基因。本研究通过对宫颈癌、
文摘Objective. Co- expression patterns of the genes implicated in angiogenesis and tumor invasion in cervical carcinoma cells were investigated together with invasive activity of tumor cells. Transcript levels of those genes were also compared between tumor cells and normal cervical tissues. Methods. Real-time quantitative RT- PCR analysis was conducted on selected 11 genes (total VEGF- A, VEGF121,VEGF165,VEGF189,VEGF- B, C and D, bFGF, dThdPase, MMP- 2 and uPA) using 11 cervical carcinoma cell lines and 14 normal cervical tissues. Protein ex-pression of VEGF- C and MMP- 2 and invasive activity of tumor cells were evaluated for each cell line by sandwich ELISA and haptoinvasion assay, respectively. Results. Gene co-expression analysis revealed the significant correlation between angiogenic factors and proteinases in malignant but not in normal cervical samples. Gene or protein expression levels of VEGF- C and MMP- 2 were well correlated with the number of invaded tumor cells. VEGF- A splicing variants were increased in malignant compared to normal cervical samples but not associated with the invasive activity of the cells. Conclusion. VEGF- C and MMP- 2 were closely related to invasive phenotype of tumor cells, whereas VEGF- A isoforms were considered to be involved in cervical carcinogenesis.
