目的构建结肠腺癌和正常黏膜基因的mRNA表达谱,探讨其表达差异,为进一步研究提供线索。方法用SAGE(Serial analysis of geneexpression)方法构建两者的mRNA表达文库,挑选一定数量克隆进行测序,结果用SAGE2000软件分析并进行比较。结果...目的构建结肠腺癌和正常黏膜基因的mRNA表达谱,探讨其表达差异,为进一步研究提供线索。方法用SAGE(Serial analysis of geneexpression)方法构建两者的mRNA表达文库,挑选一定数量克隆进行测序,结果用SAGE2000软件分析并进行比较。结果构建了同一患者结肠正常黏膜和腺癌的SAGE表达文库,软件分析分别获得标签7926和7672个。癌组织与正常黏膜组织及与国外先期构建的结肠腺癌SAGE文库相比较其高表达基因之间均存在明显的表达差异。结论 SAGE技术是构建mRNA表达谱的有效方法 ;结肠腺癌与正常黏膜高表达基因之间存在明显差别;中国人结肠腺癌的基因表达可能具有自身的特点。展开更多
PURPOSE: Platelet-derived endothelial cell growth factor, identified to be an angiogenic factor, has been implicated in metastases of colorectal cancer. This study aimed to clarify the role and localization of platele...PURPOSE: Platelet-derived endothelial cell growth factor, identified to be an angiogenic factor, has been implicated in metastases of colorectal cancer. This study aimed to clarify the role and localization of platelet-derived endothelial cell growth factor associated with human colorectal cancer invasion. METHODS: Thirty-two patients with colorectal cancer who had undergone surgery were analyzed. Platelet-derived endothelial cell growth factor enzyme activities in the colorectal cancer specimens were measured. Cells that expressed platelet-derived endothelial cell growth factor were identified and localized by immunohistochemical analysis with anti-human platelet-derived endothelial cell growth factor antibody and by in situ hybridization with specific RNA probe. RESULTS: Platelet-derived endothelial cell growth factor enzyme activity increased significantly in cancer tissues compared with normal colonic mucosa at various distances from the cancer. Immunohistochemical analysis and in situ hybridization demonstrated platelet-derived endothelial cell growth factor expression in stromal macrophages and fibroblasts located in cancer tissues and surrounding noncancerous tissues, although the tumor cells and normal colonic mucosa were negative. The value of platelet-derived endothelial cell growth factor expression was highest at the border of the colorectal cancer (35.3 ±8.9 percent), followed by the cancer nest (15.2 ±9.2 percent) and normal mucosa (7.7 ±3.4 percent). In the border area, the highest value of platelet-derived endothelial cell growth factor expressionwas observed in the submucosa (35.3 ±8.9 percent), followed by the muscular propria (21.9 ±7.7 percent) and the subserosa (14.9 ±5.5 percent). CONCLUSIONS: Stromal macrophages and fibroblasts are responsible for elevated platelet-derived endothelial cell growth factor activity in colorectal cancer. The significance of enhanced expression of plateletderived endothelial cell growth factor in the submucosa at the cancer border remains unclear. Cancer stroma may be an important factor for cancer angiogenesis and may serve as a treatment target through specific modulation of angiogenic factors.展开更多
文摘PURPOSE: Platelet-derived endothelial cell growth factor, identified to be an angiogenic factor, has been implicated in metastases of colorectal cancer. This study aimed to clarify the role and localization of platelet-derived endothelial cell growth factor associated with human colorectal cancer invasion. METHODS: Thirty-two patients with colorectal cancer who had undergone surgery were analyzed. Platelet-derived endothelial cell growth factor enzyme activities in the colorectal cancer specimens were measured. Cells that expressed platelet-derived endothelial cell growth factor were identified and localized by immunohistochemical analysis with anti-human platelet-derived endothelial cell growth factor antibody and by in situ hybridization with specific RNA probe. RESULTS: Platelet-derived endothelial cell growth factor enzyme activity increased significantly in cancer tissues compared with normal colonic mucosa at various distances from the cancer. Immunohistochemical analysis and in situ hybridization demonstrated platelet-derived endothelial cell growth factor expression in stromal macrophages and fibroblasts located in cancer tissues and surrounding noncancerous tissues, although the tumor cells and normal colonic mucosa were negative. The value of platelet-derived endothelial cell growth factor expression was highest at the border of the colorectal cancer (35.3 ±8.9 percent), followed by the cancer nest (15.2 ±9.2 percent) and normal mucosa (7.7 ±3.4 percent). In the border area, the highest value of platelet-derived endothelial cell growth factor expressionwas observed in the submucosa (35.3 ±8.9 percent), followed by the muscular propria (21.9 ±7.7 percent) and the subserosa (14.9 ±5.5 percent). CONCLUSIONS: Stromal macrophages and fibroblasts are responsible for elevated platelet-derived endothelial cell growth factor activity in colorectal cancer. The significance of enhanced expression of plateletderived endothelial cell growth factor in the submucosa at the cancer border remains unclear. Cancer stroma may be an important factor for cancer angiogenesis and may serve as a treatment target through specific modulation of angiogenic factors.
