目的:比较生蒲黄与炒蒲黄、蒲黄炭提取物对正常BALB/c小鼠非特异性免疫功能的影响。方法:将正常BALB/c雄性小鼠,随机分为4组,即空白对照组、生蒲黄组、炒蒲黄组、蒲黄炭组。各药物组分别按0.15 m L/10g体重每天灌胃1次,连续15 d。空白...目的:比较生蒲黄与炒蒲黄、蒲黄炭提取物对正常BALB/c小鼠非特异性免疫功能的影响。方法:将正常BALB/c雄性小鼠,随机分为4组,即空白对照组、生蒲黄组、炒蒲黄组、蒲黄炭组。各药物组分别按0.15 m L/10g体重每天灌胃1次,连续15 d。空白对照组给予等体积蒸馏水。通过对正常BALB/c小鼠免疫器官指数、炭粒廓清实验、血清溶菌酶含量等非特异性免疫指标来检测上述药物对其影响。结果:生蒲黄对正常BALB/c小鼠无非特异性免疫作用;炒蒲黄、蒲黄炭组分别通过促进溶菌酶分泌、增加胸腺指数来增强免疫作用。结论:炒蒲黄、蒲黄炭对正常BALB/c小鼠具有增强非特异性免疫的作用。展开更多
目的观察电针对正常高值血压盐敏感大鼠肾脏内质网应激时GRP78、CHOP及肾细胞凋亡的影响,探讨电针调节内质网应激保护肾脏的作用机制。方法将24只7周龄雄性Dahl盐敏感大鼠(Dahl salt sensitive rat,DS)按随机数字表法随机分为模型组、...目的观察电针对正常高值血压盐敏感大鼠肾脏内质网应激时GRP78、CHOP及肾细胞凋亡的影响,探讨电针调节内质网应激保护肾脏的作用机制。方法将24只7周龄雄性Dahl盐敏感大鼠(Dahl salt sensitive rat,DS)按随机数字表法随机分为模型组、针刺组、非经非穴组。另将8只同龄雄性盐抵抗大鼠(Dahl salt resistant rats,DR)设为空白组。采用8%高盐饲料喂养法制备正常高值血压模型。正常高值血压大鼠模型为BP130~139/85~89 mm Hg。造模成功后改为普通饲料喂养。空白组、模型组每天捆绑固定20 min,不作其他干预;针刺组予双侧足三里、曲池电针治疗20 min;非经非穴组在双侧髂脊上10~15 mm、后正中线旁开20 mm区段内选择1个固定对照点电针治疗20 min。每日1次,每周6天,休息1 d,连续治疗4周。取大鼠肾组织,采用荧光定量PCR法和蛋白免疫印迹法(Western bolt)检测GRP78、CHOP的表达,采用TUNEL检测肾细胞凋亡水平。结果干预4周后,与模型组比较,针刺组血压显著降低(P<0.05),非经非穴组血压差异无统计学意义(P>0.05);针刺组GRP78、CHOP的mRNA和蛋白表达及肾细胞凋亡率显著降低(P<0.05)。与针刺组比较,非经非穴组GRP78、CHOP的mRNA和蛋白表达及肾细胞凋亡率差异有统计学意义(P<0.05)。结论电针足三里、曲池可能通过抑制内质网应激,抑制肾细胞凋亡,对正常高值血压DS大鼠肾脏起到保护作用。展开更多
AIM: To study the sensitivity of gastric smooth muscle to C-type natriuretic peptide (CNP) in streptozotocin (STZ)-induced diabetic rats. METHODS: The spontaneous contraction of a gastric smooth muscle strip was recor...AIM: To study the sensitivity of gastric smooth muscle to C-type natriuretic peptide (CNP) in streptozotocin (STZ)-induced diabetic rats. METHODS: The spontaneous contraction of a gastric smooth muscle strip was recorded by using physiological methods in rats. The expressions of CNP and natriuretic peptide receptor-B (NPR-B) in gastric tissue were examined by using immunohistochemistry techniques in the diabetic rat. RESULTS: At 4 wk after injection of STZ and vehicle, the frequency of spontaneous contraction of gastric smooth muscle was significantly reduced in diabetic rats, and the frequency was decreased from 3.10 ± 0.14 cycle/min in controls to 2.23 ± 0.13 cycle/min (n = 8, P < 0.01). However, the amplitude of spontaneous contraction was not significant different from the normal rat. CNP significantly inhibited spontaneous contraction of gastric smooth muscle in normal and diabetic rats, but the inhibitory effect was significantly potentiated in the diabetic rats. The amplitudes of spontaneous contraction were suppressed by 75.15% ± 0.71% and 58.92% ± 1.32% while the frequencies were decreased by 53.33% ± 2.03% and 26.95% ± 2.82% in diabetic and normal rats, respectively (n = 8, P < 0.01). The expression of CNP in gastric tissue was not changed in diabetic rats, however the expression of NPR-B was significantly increased in diabetic rats, and the staining indexes of NPR-B were 30.67 ± 1.59 and 17.63 ± 1.49 in diabetic and normal rat, respectively (n = 8, P < 0.01).CONCLUSION: The results suggest that CNP induced an inhibitory effect on spontaneous contraction of gastric smooth muscle, potentiated in diabetic rat via up-regulation of the natriuretic peptides-NPR-B-particulate guanylyl cyclase-cyclic GMP signal pathway.展开更多
文摘目的:比较生蒲黄与炒蒲黄、蒲黄炭提取物对正常BALB/c小鼠非特异性免疫功能的影响。方法:将正常BALB/c雄性小鼠,随机分为4组,即空白对照组、生蒲黄组、炒蒲黄组、蒲黄炭组。各药物组分别按0.15 m L/10g体重每天灌胃1次,连续15 d。空白对照组给予等体积蒸馏水。通过对正常BALB/c小鼠免疫器官指数、炭粒廓清实验、血清溶菌酶含量等非特异性免疫指标来检测上述药物对其影响。结果:生蒲黄对正常BALB/c小鼠无非特异性免疫作用;炒蒲黄、蒲黄炭组分别通过促进溶菌酶分泌、增加胸腺指数来增强免疫作用。结论:炒蒲黄、蒲黄炭对正常BALB/c小鼠具有增强非特异性免疫的作用。
基金Supported by The National Natural Science Foundation of China, No. 30760068
文摘AIM: To study the sensitivity of gastric smooth muscle to C-type natriuretic peptide (CNP) in streptozotocin (STZ)-induced diabetic rats. METHODS: The spontaneous contraction of a gastric smooth muscle strip was recorded by using physiological methods in rats. The expressions of CNP and natriuretic peptide receptor-B (NPR-B) in gastric tissue were examined by using immunohistochemistry techniques in the diabetic rat. RESULTS: At 4 wk after injection of STZ and vehicle, the frequency of spontaneous contraction of gastric smooth muscle was significantly reduced in diabetic rats, and the frequency was decreased from 3.10 ± 0.14 cycle/min in controls to 2.23 ± 0.13 cycle/min (n = 8, P < 0.01). However, the amplitude of spontaneous contraction was not significant different from the normal rat. CNP significantly inhibited spontaneous contraction of gastric smooth muscle in normal and diabetic rats, but the inhibitory effect was significantly potentiated in the diabetic rats. The amplitudes of spontaneous contraction were suppressed by 75.15% ± 0.71% and 58.92% ± 1.32% while the frequencies were decreased by 53.33% ± 2.03% and 26.95% ± 2.82% in diabetic and normal rats, respectively (n = 8, P < 0.01). The expression of CNP in gastric tissue was not changed in diabetic rats, however the expression of NPR-B was significantly increased in diabetic rats, and the staining indexes of NPR-B were 30.67 ± 1.59 and 17.63 ± 1.49 in diabetic and normal rat, respectively (n = 8, P < 0.01).CONCLUSION: The results suggest that CNP induced an inhibitory effect on spontaneous contraction of gastric smooth muscle, potentiated in diabetic rat via up-regulation of the natriuretic peptides-NPR-B-particulate guanylyl cyclase-cyclic GMP signal pathway.
