Apoptosis plays a pivotal role in the etiology or pathogenesis of numerous medical disorders, and thus, target- ing of apoptotic cells may substantially advance patient care. In our quest for novel low-molecular-weigh...Apoptosis plays a pivotal role in the etiology or pathogenesis of numerous medical disorders, and thus, target- ing of apoptotic cells may substantially advance patient care. In our quest for novel low-molecular-weight probes for apoptosis, we focused on the uncommon amino acid T-carboxyglutamic acid （Gla）, which plays a vital role in the binding of clotting factors to negatively charged phospholipid surfaces. Based on the alkyl-malonic acid motif of Gla, we have developed and now present ML-10 （2-（5-fluoro-pentyl）-2-methyl-malonic acid, MW=206 Da）, the pro- totypical member of a novel family of small-molecule detectors of apoptosis. ML-10 was found to perform selective uptake and accumulation in apoptotic cells, while being excluded from either viable or necrotic cells. ML-10 uptake correlates with the apoptotic hallmarks of caspase activation, Annexin-V binding and disruption of mitochondrial membrane potential. The malonate moiety was found to be crucial for ML-10 function in apoptosis detection. ML- 10 responds to a unique complex of features of the cell in early apoptosis, comprising irreversible loss of membrane potential, permanent acidification of cell membrane and cytoplasm, and preservation of membrane integrity. ML-10 is therefore the most compact apoptosis probe known to date. Due to its fluorine atom, ML-10 is amenable to radio- labeling with the lSF isotope, towards its potential future use for clinical positron emission tomography imaging of apoptosis.展开更多
In this paper, a mathematical model about the optimal booking level problem in airline overbooking rnanageanent is proposed. Firstly, the normal distribution is employed to approximate Poisson requests and binomial go...In this paper, a mathematical model about the optimal booking level problem in airline overbooking rnanageanent is proposed. Firstly, the normal distribution is employed to approximate Poisson requests and binomial goshows. By calculating the mean loss, a model to describe the relationship between booking level and flight revenue is established. Then, an equation is deduced to decide the optimal booking level. Finally, a numerical example is utilized to illustrate the proposed model and discuss the impact of refund rate on the optimal booking level and the mean loss.展开更多
In order to accurately predict the productivity of herringbone multilateral well,a new productivity prediction model was founded.And based on this model,orthogonal test and multiple factor variance analysis were appli...In order to accurately predict the productivity of herringbone multilateral well,a new productivity prediction model was founded.And based on this model,orthogonal test and multiple factor variance analysis were applied to study optimization design of herringbone multilateral well.According to the characteristics of herringbone multilateral well,by using pressure superposition and mirror image reflection theory,the coupled model of herringbone multilateral well was developed on the basis of a three-dimensional pseudo-pressure distribution model for horizontal wells.The model was formulated in consideration of friction loss,acceleration loss of the wellbore and mixed loss at the confluence of main wellbore and branched one.After mathematical simulation on productivity of the herringbone multilateral well with the coupled model,the effects of well configuration on productivity were analyzed.The results show that lateral number is the most important factor,length of main wellbore and length of branched wellbore are the secondary ones,angle between main and branched one has the least influence.展开更多
Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the...Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.展开更多
Nutrient resorption is a crucial mechanism for plant nutrient conservation,but most previous studies did not consider the leaf-mass loss during senescence due to lack of measured data.This would lead to an underestima...Nutrient resorption is a crucial mechanism for plant nutrient conservation,but most previous studies did not consider the leaf-mass loss during senescence due to lack of measured data.This would lead to an underestimation of nutrient resorption efficiency(NuRE),or calculating NuRE of various species based on the average mass loss at plant-functional-group level in the literature,thus affecting its accuracy.Here we measured the leaf-mass loss to correct NuRE with the species-specific mass loss correction factor(MLCF),so as to foster a more accurate calculation of the nutrient fluxes within and between plants and the soil.Green leaves and senesced leaves were collected from 35 dominant woody plants in northern China.Mass of green and senesced leaves were measured to calculate the MLCF at species level.The MLCF was reported for each of the 35 dominant woody plants in northern China.These species averagely lost 17%of the green-leaf mass during leaf senescence,but varied greatly from 1.3%to 36.8%mass loss across the 35 species,or 11.7%to 19.6%loss across the functional types.Accordingly,the MLCF varied from 0.632 to 0.987 across the 35 species with an average value 0.832.The NuRE corrected with MLCF was remarkably increased on the whole(e.g.both the average nitrogen and phosphorus NuRE became about 9%higher,or more accurate),compared with the uncorrected ones,especially in the case of low resorption efficiencies.Our field data provide reliable references for the MLCF of plants in related regions at both species and functional-type levels,and are expected to promote more accurate calculations of NuRE.展开更多
We consider an Error-in-Variable partially linear model where the covariates of linear part are measured with error which follows a normal distribution with a known covariance matrix. We propose a corrected-loss estim...We consider an Error-in-Variable partially linear model where the covariates of linear part are measured with error which follows a normal distribution with a known covariance matrix. We propose a corrected-loss estimation of the covariate effect. The proposed estimator is asymptotically normal. Simulation studies are presented to show that the proposed method performs well with finite samples, and the proposed method is applied to a real data set.展开更多
文摘Apoptosis plays a pivotal role in the etiology or pathogenesis of numerous medical disorders, and thus, target- ing of apoptotic cells may substantially advance patient care. In our quest for novel low-molecular-weight probes for apoptosis, we focused on the uncommon amino acid T-carboxyglutamic acid （Gla）, which plays a vital role in the binding of clotting factors to negatively charged phospholipid surfaces. Based on the alkyl-malonic acid motif of Gla, we have developed and now present ML-10 （2-（5-fluoro-pentyl）-2-methyl-malonic acid, MW=206 Da）, the pro- totypical member of a novel family of small-molecule detectors of apoptosis. ML-10 was found to perform selective uptake and accumulation in apoptotic cells, while being excluded from either viable or necrotic cells. ML-10 uptake correlates with the apoptotic hallmarks of caspase activation, Annexin-V binding and disruption of mitochondrial membrane potential. The malonate moiety was found to be crucial for ML-10 function in apoptosis detection. ML- 10 responds to a unique complex of features of the cell in early apoptosis, comprising irreversible loss of membrane potential, permanent acidification of cell membrane and cytoplasm, and preservation of membrane integrity. ML-10 is therefore the most compact apoptosis probe known to date. Due to its fluorine atom, ML-10 is amenable to radio- labeling with the lSF isotope, towards its potential future use for clinical positron emission tomography imaging of apoptosis.
文摘In this paper, a mathematical model about the optimal booking level problem in airline overbooking rnanageanent is proposed. Firstly, the normal distribution is employed to approximate Poisson requests and binomial goshows. By calculating the mean loss, a model to describe the relationship between booking level and flight revenue is established. Then, an equation is deduced to decide the optimal booking level. Finally, a numerical example is utilized to illustrate the proposed model and discuss the impact of refund rate on the optimal booking level and the mean loss.
基金Project(12521044) supported by Scientific and Technological Research Program of Heilongjiang Provincial Education Department,China
文摘In order to accurately predict the productivity of herringbone multilateral well,a new productivity prediction model was founded.And based on this model,orthogonal test and multiple factor variance analysis were applied to study optimization design of herringbone multilateral well.According to the characteristics of herringbone multilateral well,by using pressure superposition and mirror image reflection theory,the coupled model of herringbone multilateral well was developed on the basis of a three-dimensional pseudo-pressure distribution model for horizontal wells.The model was formulated in consideration of friction loss,acceleration loss of the wellbore and mixed loss at the confluence of main wellbore and branched one.After mathematical simulation on productivity of the herringbone multilateral well with the coupled model,the effects of well configuration on productivity were analyzed.The results show that lateral number is the most important factor,length of main wellbore and length of branched wellbore are the secondary ones,angle between main and branched one has the least influence.
文摘Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.
基金TheNational Key Researchand Development Program of China(2018YFC0507204)"Strategic Priority Research Program"of the Chinese Academy of Sciences(XDA26040202)the National Natural Science Foundation of China(41473068).
文摘Nutrient resorption is a crucial mechanism for plant nutrient conservation,but most previous studies did not consider the leaf-mass loss during senescence due to lack of measured data.This would lead to an underestimation of nutrient resorption efficiency(NuRE),or calculating NuRE of various species based on the average mass loss at plant-functional-group level in the literature,thus affecting its accuracy.Here we measured the leaf-mass loss to correct NuRE with the species-specific mass loss correction factor(MLCF),so as to foster a more accurate calculation of the nutrient fluxes within and between plants and the soil.Green leaves and senesced leaves were collected from 35 dominant woody plants in northern China.Mass of green and senesced leaves were measured to calculate the MLCF at species level.The MLCF was reported for each of the 35 dominant woody plants in northern China.These species averagely lost 17%of the green-leaf mass during leaf senescence,but varied greatly from 1.3%to 36.8%mass loss across the 35 species,or 11.7%to 19.6%loss across the functional types.Accordingly,the MLCF varied from 0.632 to 0.987 across the 35 species with an average value 0.832.The NuRE corrected with MLCF was remarkably increased on the whole(e.g.both the average nitrogen and phosphorus NuRE became about 9%higher,or more accurate),compared with the uncorrected ones,especially in the case of low resorption efficiencies.Our field data provide reliable references for the MLCF of plants in related regions at both species and functional-type levels,and are expected to promote more accurate calculations of NuRE.
基金supported by National Natural Science Foundation of China(Grant Nos.10901020 and 11371062)the Fundamental Research Funds for the Central Universities,Beijing Center for Mathematics and Information Interdisciplinary Sciences,China Zhongdian Project(Grant No.11131002)
文摘We consider an Error-in-Variable partially linear model where the covariates of linear part are measured with error which follows a normal distribution with a known covariance matrix. We propose a corrected-loss estimation of the covariate effect. The proposed estimator is asymptotically normal. Simulation studies are presented to show that the proposed method performs well with finite samples, and the proposed method is applied to a real data set.
