AIM: To investigate the effect of vascular endothelial growth factor (VEGF) transfection on hepatic sinusoidal capillarization. METHODS: Enhanced green fluorescent protein (EGFP)/ VEGF transfection was confirmed by im...AIM: To investigate the effect of vascular endothelial growth factor (VEGF) transfection on hepatic sinusoidal capillarization. METHODS: Enhanced green fluorescent protein (EGFP)/ VEGF transfection was confirmed by immunofluorescence microscopy and immunohistoche-mistry both in primary hepatocytes and in normal liver. Cirrhotic rats were generated by thioacetamide (TAA) administration and then divided into a treatment group, which received injections of 400 μg of plasmid DNA encoding an EGFP- VEGF fusion protein, and a blank group, which received an equal amount of normal saline through the portal vein. The portal vein pressure was measured in the normal and cirrhotic state, in treated and blank groups. The average number of fenestrae per hepatic sinusoid was determined using transmission electron microscopy (TEM), while the relative abundance of VEGF transcripts was examined by Gene array. RESULTS: Green fluorescent protein was observed in the cytoplasms of liver cells under immunofluorescence microscopy 24 h after transfection with EGFP/VEGF plasmid in vitro. Staining with polyclonal antibodies against VEGF illustrated that hepatocytes expressedimmunodetectable VEGF both in vitro and in vitro. There were significant differences in the number of fenestrae and portal vein pressures between normal and cirrhotic rats (7.40 ± 1.71 vs 2.30 ± 1.16 and 9.32 ± 0.85 cmH2O vs 17.92 ± 0.90 cmH2O, P < 0.01), between cirrhotic and treated rats (2.30 ± 1.16 cmH2O vs 4.60 ± 1.65 and 17.92 ± 0.90 cmH2O vs 15.52 ± 0.93 cmH2O, P < 0.05) and between the treatment group and the blank group (4.60 ± 1.65 cmH2O vs 2.10 ± 1.10 cmH2O and 15.52 ± 0.93 cmH2O vs 17.26 ± 1.80 cmH2O, P < 0.05). Gene- array analysis revealed that the relative abundance of transcripts of VEGF family members decreased in the cirrhotic state and increased after transfection. CONCLUSION: Injection of a plasmid encoding VEGF through the portal vein is an effective method to induce the formation of fenestrae and decrease portal vein pressure in cirrhotic rats. Therefore, it may be a good choice for treating hepatic cirrhosis and portal hypertension.展开更多
During the process of coal prospecting and exploration, different measurement time, different logging instruments and series can lead to systematic errors in well logs. Accordingly, all logging curves need to be norma...During the process of coal prospecting and exploration, different measurement time, different logging instruments and series can lead to systematic errors in well logs. Accordingly, all logging curves need to be normalized in the mining area. By studying well-logging normalization methods, and focusing on the characteristics of the coalfield, the frequency histogram method was used in accordance with the condition of the Guqiao Coal Mine. In this way, the density and sonic velocity at marker bed in the non-key well were made to close to those in the key well, and were eventually equal. Well log normalization was completed when this method was applied to the entire logging curves. The results show that the scales of logging data were unified by normalizing coal logging curves, and the logging data were consistent with wave impedance inversion data. A satisfactory inversion effect was obtained.展开更多
The Bézier curve is one of the most commonly used parametric curves in CAGD and Computer Graphics and has many good properties for shape design. Developing more convenient techniques for designing and modifying B...The Bézier curve is one of the most commonly used parametric curves in CAGD and Computer Graphics and has many good properties for shape design. Developing more convenient techniques for designing and modifying Bézier curve is an im- portant problem, and is also an important research issue in CAD/CAM and NC technology fields. This work investigates the optimal shape modification of Bézier curves by geometric constraints. This paper presents a new method by constrained optimi- zation based on changing the control points of the curves. By this method, the authors modify control points of the original Bézier curves to satisfy the given constraints and modify the shape of the curves optimally. Practical examples are also given.展开更多
Making use of a new generalized ans?tze and a proper transformation, we generalized the extended tanh-function method. Applying the generalized method with the aid of Maple, we consider some nonlinear evolution equati...Making use of a new generalized ans?tze and a proper transformation, we generalized the extended tanh-function method. Applying the generalized method with the aid of Maple, we consider some nonlinear evolution equations. As a result, we can successfully recover the previously known solitary wave solutions that had been found by the extended tanh-function method and other more sophisticated methods. More importantly, for some equations, we also obtain other new and more general solutions at the same time. The results include kink-profile solitary-wave solutions, bell-profile solitary-wave solutions, periodic wave solutions, rational solutions, singular solutions and new formal solutions.展开更多
Is the paper [2] the authors defined the singular Darboux transformations and established an explicit formula for constructing unitons from a simply connected Riemann surface. M to the group U(N). The formula is obtai...Is the paper [2] the authors defined the singular Darboux transformations and established an explicit formula for constructing unitons from a simply connected Riemann surface. M to the group U(N). The formula is obtained as a limit of an infinite consequence of Darboux transformations through some renormalization procedure. In the present paper the authors give a complete proof of the fact that the formula gives a global solution of harmonic maps without singularity.展开更多
We prove that any abelian cover over a smooth variety is defined by some cyclic equations. From the defining equations, we compute explicitly the normalization, branch locus, ramification indices, global invariants, a...We prove that any abelian cover over a smooth variety is defined by some cyclic equations. From the defining equations, we compute explicitly the normalization, branch locus, ramification indices, global invariants, and the resolution of singularities. As an application, we construct a new algebraic surface which is the quotient of ball.展开更多
基金The National Natural Science Fundation of China, No. 30300341The Natural Science Fundation of Shandong Province, No. Y2004Z12
文摘AIM: To investigate the effect of vascular endothelial growth factor (VEGF) transfection on hepatic sinusoidal capillarization. METHODS: Enhanced green fluorescent protein (EGFP)/ VEGF transfection was confirmed by immunofluorescence microscopy and immunohistoche-mistry both in primary hepatocytes and in normal liver. Cirrhotic rats were generated by thioacetamide (TAA) administration and then divided into a treatment group, which received injections of 400 μg of plasmid DNA encoding an EGFP- VEGF fusion protein, and a blank group, which received an equal amount of normal saline through the portal vein. The portal vein pressure was measured in the normal and cirrhotic state, in treated and blank groups. The average number of fenestrae per hepatic sinusoid was determined using transmission electron microscopy (TEM), while the relative abundance of VEGF transcripts was examined by Gene array. RESULTS: Green fluorescent protein was observed in the cytoplasms of liver cells under immunofluorescence microscopy 24 h after transfection with EGFP/VEGF plasmid in vitro. Staining with polyclonal antibodies against VEGF illustrated that hepatocytes expressedimmunodetectable VEGF both in vitro and in vitro. There were significant differences in the number of fenestrae and portal vein pressures between normal and cirrhotic rats (7.40 ± 1.71 vs 2.30 ± 1.16 and 9.32 ± 0.85 cmH2O vs 17.92 ± 0.90 cmH2O, P < 0.01), between cirrhotic and treated rats (2.30 ± 1.16 cmH2O vs 4.60 ± 1.65 and 17.92 ± 0.90 cmH2O vs 15.52 ± 0.93 cmH2O, P < 0.05) and between the treatment group and the blank group (4.60 ± 1.65 cmH2O vs 2.10 ± 1.10 cmH2O and 15.52 ± 0.93 cmH2O vs 17.26 ± 1.80 cmH2O, P < 0.05). Gene- array analysis revealed that the relative abundance of transcripts of VEGF family members decreased in the cirrhotic state and increased after transfection. CONCLUSION: Injection of a plasmid encoding VEGF through the portal vein is an effective method to induce the formation of fenestrae and decrease portal vein pressure in cirrhotic rats. Therefore, it may be a good choice for treating hepatic cirrhosis and portal hypertension.
基金Supported by the National Basic Research Program of China (2009CB219603, 2010CB226800) the National Natural Science Foundation of China (40874071, 40672104)
文摘During the process of coal prospecting and exploration, different measurement time, different logging instruments and series can lead to systematic errors in well logs. Accordingly, all logging curves need to be normalized in the mining area. By studying well-logging normalization methods, and focusing on the characteristics of the coalfield, the frequency histogram method was used in accordance with the condition of the Guqiao Coal Mine. In this way, the density and sonic velocity at marker bed in the non-key well were made to close to those in the key well, and were eventually equal. Well log normalization was completed when this method was applied to the entire logging curves. The results show that the scales of logging data were unified by normalizing coal logging curves, and the logging data were consistent with wave impedance inversion data. A satisfactory inversion effect was obtained.
基金Project (No.10471128) supported by the National Natural ScienceFoundation of China
文摘The Bézier curve is one of the most commonly used parametric curves in CAGD and Computer Graphics and has many good properties for shape design. Developing more convenient techniques for designing and modifying Bézier curve is an im- portant problem, and is also an important research issue in CAD/CAM and NC technology fields. This work investigates the optimal shape modification of Bézier curves by geometric constraints. This paper presents a new method by constrained optimi- zation based on changing the control points of the curves. By this method, the authors modify control points of the original Bézier curves to satisfy the given constraints and modify the shape of the curves optimally. Practical examples are also given.
文摘Making use of a new generalized ans?tze and a proper transformation, we generalized the extended tanh-function method. Applying the generalized method with the aid of Maple, we consider some nonlinear evolution equations. As a result, we can successfully recover the previously known solitary wave solutions that had been found by the extended tanh-function method and other more sophisticated methods. More importantly, for some equations, we also obtain other new and more general solutions at the same time. The results include kink-profile solitary-wave solutions, bell-profile solitary-wave solutions, periodic wave solutions, rational solutions, singular solutions and new formal solutions.
基金he Special Funds for Major State Basic Ressarch Project of China (NonlinearScience), the National Natural Science Foundation o
文摘Is the paper [2] the authors defined the singular Darboux transformations and established an explicit formula for constructing unitons from a simply connected Riemann surface. M to the group U(N). The formula is obtained as a limit of an infinite consequence of Darboux transformations through some renormalization procedure. In the present paper the authors give a complete proof of the fact that the formula gives a global solution of harmonic maps without singularity.
基金supported by National Natural Science Foundation of China (Grant No.10731030)the Innovation Program of Shanghai Municipal Education Commission (Grant No. 11ZZ18)
文摘We prove that any abelian cover over a smooth variety is defined by some cyclic equations. From the defining equations, we compute explicitly the normalization, branch locus, ramification indices, global invariants, and the resolution of singularities. As an application, we construct a new algebraic surface which is the quotient of ball.