Several groups of direct shear tests of Nanning expansive soil samples were carded out by improved direct shear apparatus. The results of the characteristics of the ultimate shear stress and residual shear stress at t...Several groups of direct shear tests of Nanning expansive soil samples were carded out by improved direct shear apparatus. The results of the characteristics of the ultimate shear stress and residual shear stress at the interface of expansive soil-structure are presented as follows： linear relation can approximately reflect changes between the both shear stress and the three factors： vertical load, water content and dry density, just different degrees from each other; increasing the vertical load from 25 kPa to 100 kPa （up by 300%） can cause the average increase of ultimate shear stress from 58% （for samples with 1.61 g/cm^3） to 80% （for samples with 1.76 g/cm^3）, and an close average increase of 180% for the residual shear stress; increasing the water content from 14.1% to 20.8% （up by 47.5%） can cause the average decrease of the ultimate shear stress from 40% （for samples with 25 kPa） to 80% （for samples with 100 kPa）, and the average decrease from 25% （for samples with 25 kPa） to 30% （for samples with 100 kPa） for the residual shear stress; increasing the dry density from 1.61 g/cm^3 to 1.76 g/cm^3 （up by 9.3%） can cause the average increase of ultimate shear stress from 92% （for samples with 25 kPa） to 138% （for samples with 100 kPa）, and an average increase of 4% for the residual shear stress. Sensitive analysis was further made to explain reasons causing the differences of the both shear stress induced by the three factors.展开更多
The design of variants to enhance conformational stability of proteins is an important aspect of protein engineering. Oligomeric proteins are often stabilized by aromatic clusters located within the subunit interfaces...The design of variants to enhance conformational stability of proteins is an important aspect of protein engineering. Oligomeric proteins are often stabilized by aromatic clusters located within the subunit interfaces. In the present study, the authors constructed five variants of Ps3aHSD （Pseudomonas sp. B-0831 3a-hydroxysteroid dehydrogenase） in which one or two residues at the dimer interface were replaced with aromatic residues, and examined the effects of introducing aromatic residues in this region on protein thermostability. Under their experimental conditions, all variants formed dimers, similar to wild-type Ps3aHSD. Thermal denaturation experiments indicated that Tm of all variants was 0.2-16.2 °C lower than that of wild-type protein, indicating less stable thanwild-type protein. The results collectively suggest that aromatic residues of natural oligomeric proteins are strictly posted in the interface to facilitate optimal interactions and avoid conformational strain.展开更多
基金Projects(50378097 50678177) supported by the National Natural Science Foundation of ChinaProject (01JJY3043) supported by the Natural Science Foundation of Hunan Province, China
文摘Several groups of direct shear tests of Nanning expansive soil samples were carded out by improved direct shear apparatus. The results of the characteristics of the ultimate shear stress and residual shear stress at the interface of expansive soil-structure are presented as follows： linear relation can approximately reflect changes between the both shear stress and the three factors： vertical load, water content and dry density, just different degrees from each other; increasing the vertical load from 25 kPa to 100 kPa （up by 300%） can cause the average increase of ultimate shear stress from 58% （for samples with 1.61 g/cm^3） to 80% （for samples with 1.76 g/cm^3）, and an close average increase of 180% for the residual shear stress; increasing the water content from 14.1% to 20.8% （up by 47.5%） can cause the average decrease of the ultimate shear stress from 40% （for samples with 25 kPa） to 80% （for samples with 100 kPa）, and the average decrease from 25% （for samples with 25 kPa） to 30% （for samples with 100 kPa） for the residual shear stress; increasing the dry density from 1.61 g/cm^3 to 1.76 g/cm^3 （up by 9.3%） can cause the average increase of ultimate shear stress from 92% （for samples with 25 kPa） to 138% （for samples with 100 kPa）, and an average increase of 4% for the residual shear stress. Sensitive analysis was further made to explain reasons causing the differences of the both shear stress induced by the three factors.
文摘The design of variants to enhance conformational stability of proteins is an important aspect of protein engineering. Oligomeric proteins are often stabilized by aromatic clusters located within the subunit interfaces. In the present study, the authors constructed five variants of Ps3aHSD （Pseudomonas sp. B-0831 3a-hydroxysteroid dehydrogenase） in which one or two residues at the dimer interface were replaced with aromatic residues, and examined the effects of introducing aromatic residues in this region on protein thermostability. Under their experimental conditions, all variants formed dimers, similar to wild-type Ps3aHSD. Thermal denaturation experiments indicated that Tm of all variants was 0.2-16.2 °C lower than that of wild-type protein, indicating less stable thanwild-type protein. The results collectively suggest that aromatic residues of natural oligomeric proteins are strictly posted in the interface to facilitate optimal interactions and avoid conformational strain.
