Objective: To study the incidence of mycoplasmagenitalium infection in non-gonococcal urethritis(NGU)/mucopurulent cervicitis (MPC) patients.Method: Polymerase chain reaction (PCR) wasconducted to detect M. genitalium...Objective: To study the incidence of mycoplasmagenitalium infection in non-gonococcal urethritis(NGU)/mucopurulent cervicitis (MPC) patients.Method: Polymerase chain reaction (PCR) wasconducted to detect M. genitalium in the urogenitaltracts of 236 patients with NGU/MPC.Results: There was a specific M. genitalium band in42 out of 236 STD patients who were positive for M.genitalium by PCR.Conclusion: The results indicate that mycoplasmagenitalium exists among sexually transmitted diseasepatients. It may be one of the etiological agents of NGU/MPC.展开更多
Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid ...Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pneumoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitaliurn to host cells. The prokaryotic expression vector pET-30 （ ＋ ）/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immuno- blotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were performed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.展开更多
Objectives: To evaluate thc efficacy of nested polymerasechain reaction (PCR) with first void urine (FVU) for thediagnosis of Mycoplasma hominis in male patients. Methods: Matched FVU specimens and urethral swabs were...Objectives: To evaluate thc efficacy of nested polymerasechain reaction (PCR) with first void urine (FVU) for thediagnosis of Mycoplasma hominis in male patients. Methods: Matched FVU specimens and urethral swabs werecollected from 194 male patients with NongonococcalUrethritis and tested by nested PCR and cell culture. Cellculture was used as a gold standard for evaluating other assaytechniques. Results: For FVU nested PCR assay and FVU cell culture,our results showed that the sensitivity was 100% and 93.3%;specificity was 97.0% and 98.2%; positive predictive value(PPV) was 85.7% and 90.3%, negative predictive value (NPV)was 100% and 98.8%, respectively. The total consistencybetween the two techniques was 97.4%. Conclusions: For the diagnosis of Mycoplasma hominis inmen, nested PCR detecting FVU is a highly sensitive andspecific method. First void urine can replace swab culture orPCR in terms of acceptability and feasibility.展开更多
Object: To investigate the relationship between chlamydiatrachomatis (CT) and urogenital infection. Method Positive rate of CT in patients with inflammationof urogenital tract was significantly higher than those witho...Object: To investigate the relationship between chlamydiatrachomatis (CT) and urogenital infection. Method Positive rate of CT in patients with inflammationof urogenital tract was significantly higher than those withoutinflammation(P<0.05). Result: There was statistical difference in the males nomatter they were patients with inflammation of urogenitaltract or not (P>H0.05), while there was no statistical differencein females (P>0.05). The incidence of the infection was highamong those aging from 21-50 years old. Conclusion: The clinical manifestations of CT infectionwere obscure, so we should examine CT in patients who haveno symptoms, especially in females and those of high-riskpopulation.展开更多
文摘Objective: To study the incidence of mycoplasmagenitalium infection in non-gonococcal urethritis(NGU)/mucopurulent cervicitis (MPC) patients.Method: Polymerase chain reaction (PCR) wasconducted to detect M. genitalium in the urogenitaltracts of 236 patients with NGU/MPC.Results: There was a specific M. genitalium band in42 out of 236 STD patients who were positive for M.genitalium by PCR.Conclusion: The results indicate that mycoplasmagenitalium exists among sexually transmitted diseasepatients. It may be one of the etiological agents of NGU/MPC.
基金National Natural Science Foundation of China(No.30570093).
文摘Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pneumoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitaliurn to host cells. The prokaryotic expression vector pET-30 （ ＋ ）/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immuno- blotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were performed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.
文摘Objectives: To evaluate thc efficacy of nested polymerasechain reaction (PCR) with first void urine (FVU) for thediagnosis of Mycoplasma hominis in male patients. Methods: Matched FVU specimens and urethral swabs werecollected from 194 male patients with NongonococcalUrethritis and tested by nested PCR and cell culture. Cellculture was used as a gold standard for evaluating other assaytechniques. Results: For FVU nested PCR assay and FVU cell culture,our results showed that the sensitivity was 100% and 93.3%;specificity was 97.0% and 98.2%; positive predictive value(PPV) was 85.7% and 90.3%, negative predictive value (NPV)was 100% and 98.8%, respectively. The total consistencybetween the two techniques was 97.4%. Conclusions: For the diagnosis of Mycoplasma hominis inmen, nested PCR detecting FVU is a highly sensitive andspecific method. First void urine can replace swab culture orPCR in terms of acceptability and feasibility.
文摘Object: To investigate the relationship between chlamydiatrachomatis (CT) and urogenital infection. Method Positive rate of CT in patients with inflammationof urogenital tract was significantly higher than those withoutinflammation(P<0.05). Result: There was statistical difference in the males nomatter they were patients with inflammation of urogenitaltract or not (P>H0.05), while there was no statistical differencein females (P>0.05). The incidence of the infection was highamong those aging from 21-50 years old. Conclusion: The clinical manifestations of CT infectionwere obscure, so we should examine CT in patients who haveno symptoms, especially in females and those of high-riskpopulation.
