[Objective] The morphological characters of C.lasiocarpa were studied to find its morphological responses to different water regimes and growth stretagies through different water experiences.[Method] A seeding transpl...[Objective] The morphological characters of C.lasiocarpa were studied to find its morphological responses to different water regimes and growth stretagies through different water experiences.[Method] A seeding transplanting experiment with controlled water levels was set up,and the water level was changed at the middle of the season to compare water influence at the seeding stage and later growing period.C.lasiocarpa height,leaf length,leaf number,rhizome and adventitious roots length were measured at certain time througth the growing season.[Result] Results at the thriving season indicated that C.lasiocarpa height and leaf length in the experiment were similar to that in the field,but leaf number was higher under-5 cm water level and dry-wet alternate conditions than that in the field.At later growth period,height and leaf length under constant flooding(15cm) was significantly higher than that under other culture conditions,and also that of the field investigation;while the leaf number had a trend as-5 cm water level treatment>constant flooding>dry-wet alternate>field investigation.The height and leaf length were sensitive to seeding stage water effects,and leaf number sensitive to later influences.[Conclusion] Changes of water regimes in the growing season disturbed the growth of C.lasiocarpa,could accelerate or suspend its wilt,and modified the length of life history.展开更多
Trichophyton rubrum （T. rubrum） is a common superficial fungus. Molecular and genetic studies of T. rubrum are still limited. In this paper, we report the global analysis of gene expression profiles at different gro...Trichophyton rubrum （T. rubrum） is a common superficial fungus. Molecular and genetic studies of T. rubrum are still limited. In this paper, we report the global analysis of gene expression profiles at different growth phases using cDNA microarray technology. A total of 2044 differentially expressed genes were obtained and clustered into three expression patterns. Our data confirmed previous results that many mRNAs were pre-stored in the conidia of T. rubrum. Transcriptional profiling and function analysis showed that some glycolytic enzymes share similar expression patterns and may be coregulated during the transi- tion of growth phases. Some genes involved in small GTPase signaling pathways, and in cAMP-dependent and MAPK regulation pathways were induced in response to the growth dynamics of T. rubrum. Although the detailed biological roles of these Z rubrum genes are still unknown, our results suggest that these genes may be involved in regulation mechanisms in the life cycle of the fungus.展开更多
Objective： To observe the effect of acupuncture at Jǐngjiājǐ（颈夹脊）on the repair and regeneration of cochlear hair cells of guinea pigs with sensorineural deafness.Methods： Sixty healthy guinea pigs were selec...Objective： To observe the effect of acupuncture at Jǐngjiājǐ（颈夹脊）on the repair and regeneration of cochlear hair cells of guinea pigs with sensorineural deafness.Methods： Sixty healthy guinea pigs were selected, 20 guinea pigs were randomly assigned to the normal control group（group A）, and other guinea pigs were randomly divided into model control group（group B） and acupuncture treatment group（group C） after injection with gentamicin sulfate in order to induce deafness. No intervention was given to the guinea pigs in group A and group B, and acupuncture at Jǐngjiājǐ was given to the guinea pigs in group C for 30 days. ABR threshold, DPOAE amplitudes and hair cells counting of guinea pigs in each group were recorded after intervention for 30 days.Results： After intervention for 30 days, ABR threshold in group C was significantly lower than that in group B（38.46 ±7.36 vs 82.94 ±6.47, P〈0.01）. and the DPOAE amplitudes in group C were obviously higher than that in group B（28.06 ±5.64 vs 25.23 ±5.38, P〈0.01）. The number of cochlear hair cells in group C increased significantly, over 50% of the hair cells survived, accounting for 66.67% of the observation cases. The number of cochlear hair cells in the 3 rd and 4 th gyri was close to the normal level, and plenty of proliferous sustentacular cells can be seen. Compared with group B, the number of cochlear outer hair cells in each gyrus in group C significantly increased（36.76 ±1.97 vs 28.59±2.24, P〈0.01）, indicating that acupuncture at Jǐngjiajǐ can promote the repair and regeneration of cochlear hair cells.Conclusion： Acupuncture at Jǐngjiajǐ can promote the repair and regeneration of cochlear hair cells, thus improving the hearing of guinea pigs with deafness.展开更多
基金Supported by the National Natural Science Foundation of China(41001030)the Natural Science Basic Research Plan in Shaanxi Province of China(2014JQ5194,2014JM7206)+1 种基金the Education Department of Shaanxi Province Special Research Project(12JK048415JK1386)
文摘[Objective] The morphological characters of C.lasiocarpa were studied to find its morphological responses to different water regimes and growth stretagies through different water experiences.[Method] A seeding transplanting experiment with controlled water levels was set up,and the water level was changed at the middle of the season to compare water influence at the seeding stage and later growing period.C.lasiocarpa height,leaf length,leaf number,rhizome and adventitious roots length were measured at certain time througth the growing season.[Result] Results at the thriving season indicated that C.lasiocarpa height and leaf length in the experiment were similar to that in the field,but leaf number was higher under-5 cm water level and dry-wet alternate conditions than that in the field.At later growth period,height and leaf length under constant flooding(15cm) was significantly higher than that under other culture conditions,and also that of the field investigation;while the leaf number had a trend as-5 cm water level treatment>constant flooding>dry-wet alternate>field investigation.The height and leaf length were sensitive to seeding stage water effects,and leaf number sensitive to later influences.[Conclusion] Changes of water regimes in the growing season disturbed the growth of C.lasiocarpa,could accelerate or suspend its wilt,and modified the length of life history.
基金supported by the National Natural Science Foundation of China (Grant No. 30870104)the Eleven-Fifth Mega-Scientific Project on Infectious Diseases,China (Grant Nos. 2008ZX10401-3 and 2009ZX10004-303)an intramural grant from the Institute of Pathogen Biology,Chinese Academy of Medical Sciences (Grant No. 2006IPB008)
文摘Trichophyton rubrum （T. rubrum） is a common superficial fungus. Molecular and genetic studies of T. rubrum are still limited. In this paper, we report the global analysis of gene expression profiles at different growth phases using cDNA microarray technology. A total of 2044 differentially expressed genes were obtained and clustered into three expression patterns. Our data confirmed previous results that many mRNAs were pre-stored in the conidia of T. rubrum. Transcriptional profiling and function analysis showed that some glycolytic enzymes share similar expression patterns and may be coregulated during the transi- tion of growth phases. Some genes involved in small GTPase signaling pathways, and in cAMP-dependent and MAPK regulation pathways were induced in response to the growth dynamics of T. rubrum. Although the detailed biological roles of these Z rubrum genes are still unknown, our results suggest that these genes may be involved in regulation mechanisms in the life cycle of the fungus.
基金Supported by The Education Department of Jilin Province,"12th Five-year Plan" science and technology research planning project:J.J.K.H.Z.[2015]No.33~~
文摘Objective： To observe the effect of acupuncture at Jǐngjiājǐ（颈夹脊）on the repair and regeneration of cochlear hair cells of guinea pigs with sensorineural deafness.Methods： Sixty healthy guinea pigs were selected, 20 guinea pigs were randomly assigned to the normal control group（group A）, and other guinea pigs were randomly divided into model control group（group B） and acupuncture treatment group（group C） after injection with gentamicin sulfate in order to induce deafness. No intervention was given to the guinea pigs in group A and group B, and acupuncture at Jǐngjiājǐ was given to the guinea pigs in group C for 30 days. ABR threshold, DPOAE amplitudes and hair cells counting of guinea pigs in each group were recorded after intervention for 30 days.Results： After intervention for 30 days, ABR threshold in group C was significantly lower than that in group B（38.46 ±7.36 vs 82.94 ±6.47, P〈0.01）. and the DPOAE amplitudes in group C were obviously higher than that in group B（28.06 ±5.64 vs 25.23 ±5.38, P〈0.01）. The number of cochlear hair cells in group C increased significantly, over 50% of the hair cells survived, accounting for 66.67% of the observation cases. The number of cochlear hair cells in the 3 rd and 4 th gyri was close to the normal level, and plenty of proliferous sustentacular cells can be seen. Compared with group B, the number of cochlear outer hair cells in each gyrus in group C significantly increased（36.76 ±1.97 vs 28.59±2.24, P〈0.01）, indicating that acupuncture at Jǐngjiajǐ can promote the repair and regeneration of cochlear hair cells.Conclusion： Acupuncture at Jǐngjiajǐ can promote the repair and regeneration of cochlear hair cells, thus improving the hearing of guinea pigs with deafness.
