领略水墨山水画中的“母色”魅力

水墨画以墨代色,古时就有“墨分五色”的说法,如,张彦远《历代名画记》曰:“运墨而五色具。”墨色可以分为焦、浓、重、淡、清五色,而每一种墨色又有干、湿的变化。山水画发展史中有两种主要的创作形式:以李思训、李昭道为代表的青绿山水和以王维等文人为代表的水墨山水。在寄托画家心灵和精神的山水画中,水墨山水被视为最能代表精神自由的形式,更符合老庄哲学“大道至简”的内涵,墨色也由此被赋予了“母色”之称。该文从入门阶段的水墨山水画创作角度探讨墨色,根据墨色的特性,发现墨色作为“母色”的魅力,从四个方面予以阐述,并结合山水画教学实践,增强学生的学习动力,实现“朴素为美”的审美理想。

生命的母色

生活在北方的人,对于季节的变化最敏感。这不,一进四月,草木葱郁,不用看月份牌,谁都知道春又来了。先是一丝风,细细地吹在脸上,让人有了痒的感觉,而后是一丝雨,细得像雾水洒在身上,滋润肌肤。猛一抬眼,树梢就绿了。这绿色,是生命的复苏,自然界从此又活了。据说有个云南小姑娘,第一次到北京。

涂装施工中涂料颜色调配方法

介绍了涂装施工中涂料颜色调配方法:手工调色法、电脑调色法和全自动电脑调色法,分析了各种调配方法的优缺点,并指出了各种调配方法应遵循的基本原则和影响调配颜色准确性的关键因素。

Classification of Chinese Traditional Drug-"Beimu" (Bulbus Fritillariae) by Pyrolysis High Resolution Gas Chromatography-Pattern Recognition

The combination of pyrolysis high resolution gas chromatography and pat- tern recognition techniques is a powerful tool for the classification of traditional Chinese drug.A study has been completed on 55 Beimu samples of five different geographic origins: Eastern China.Central China.South-western China,North-western China and North-eastern China.Principal component analysis and SIMCA are applied to effectively classifying the samples according to the origin of the plants.The chemical information contained in the high resolution gas chromatographic data is sufficient to characterize the geographic origin of sam- pies.

A boy with 9p+ resulting from maternal t(4;9) translocation:a case report

Increasing cases of 9p syndrome have been reported since the first description in 1970. In the present case, a extra segment of the end of chromosome 9p resulting from a maternally inherited translocation t（4; 9）（q31 ;p24） was described in a liveborn boy with mental retardation and multiple congenital anomalies. The extra part of chromosome 9p includes segment of the chromosome region 4q31→qter of his mother but deletes a small segment 9p24→pter. To our knowledge, this is the first ease described in a liveborn child. This paper also includes a review and tabulation of clinical features seen in the 34 reported eases. The new ease had most characteristics reported eases besides worried-face, fat and abnormal lower digestion tract, which is a modification and double expansion of the previous summarization on 9p syndrome.

The Application of X-STR： Two Case Reports

The usefulness of the X-chromosomal STRs （short tandem repeats） for forensic purposes seems to be restricted, but may be valuable in some paternity cases. Due to the particular mode of inheritance, X-chromosomal X-STR has the advantage in female traces identification against male contamination and in complex kinship cases, such as deficiency cases （mother-son or father-daughter）, grandparent-grandchild comparisons, half-sisters testing, etc. In this study, 4 blood samples from two paternity cases without father were examined with TYPER19 Amplification kit first, from the result, it is hard to judge the relationship between the mother-sons, then TYPERX19 kit was conducted to obtain the full profiles of the two pairs of the mother-son, from the genetypes of the samples, it can be inferred that neither of the two pairs of mother-son were in line with the kinship, which provides important evidence for the two paternity cases. All together, the X-chromosome marks included in the TYPERX19 kit can offer the possibility to solve complex kinship cases where autosomal STR markers cannot provide the information needed.

Development of a real-time PCR assay(SYBR Green I) for rapid identification and quantification of scyphomedusae Aurelia sp.1 planulae

The complicated life cycle ofAurelia spp., comprising benthic asexually-reproducing polyps and sexually-reproducing medusae, makes it hard for researchers to identify and track them, especially for early stage individuals, such as planulae. To solve this problem, we developed a real-time PCR assay （SYBR Green I） to identify planulae in both cultured and natural seawater samples. Species-specific primers targeting Aurelia sp.1 mitochondrial 16S rDNA （mr 16S rDNA） regions were designed. Using a calibration curve constructed with plasmids containing the Aurelia sp. 1 mt 16S rDNA fragment and a standard curve for planulae, the absolute number of mt 16S rDNA copies per planula was determined and from that the total number ofplanulae per sample was estimated. For the field samples, a 100-fold dilution of the sample DNA combined with a final concentration of 0.2 μg/μL BSA in the PCR reaction mixture was used to remove real- time PCR inhibitors. Samples collected in Jiaozhou Bay from July to September 2012 were subsequently analyzed using this assay. Peak Aurelia sp.1 planula abundance occurred in July 2012 at stations near Hongdao Island and Qingdao offshore; abundances were very low in August and September. The real-time PCR assay （SYBR Green I） developed here negates the need for traditional microscopic identification, which is laborious and time-consuming, and can detect and quantify jellyfish planulae in field plankton samples rapidly and specifically.

Selection of oocytes for in vitro maturation by brilliant cresyl blue staining： a study using the mouse model

Selecting oocytes that are most likely to develop is crucial for in vitro fertilization and animal cloning. Brilliant cresyl blue （BCB） staining has been used for oocyte selection in large animals, but its wider utility needs further evaluation. Mouse oocytes were divided into those stained （BCB＋） and those unstained （BCB-） according to their ooplasm BCB coloration. Chromatin configurations, cumulus cell apoptosis, cytoplasmic maturity and developmental competence were compared between the BCB＋ and BCB- oocytes. The effects of oocyte diameter, sexual maturity and gonadotropin stimulation on the competence of BCB＋ oocytes were also analyzed. In the large- and medium-size groups, BCB＋ oocytes were larger and showed more surrounded nucleoli （SN） chromatin configurations and higher frequencies of early atresia, and they also gained better cytoplasmic maturity （determined as the intracellular GSH level and pattern of mitochondrial distribution） and higher developmental potential after in vitro maturation （IVM） than the BCB-oocytes. Adult mice produced more BCB＋ oocytes with higher competence than the prepubertal mice when not primed with PMSG. PMSG priming increased both proportion and developmental potency of BCB＋ oocytes. The BCB＋ oocytes in the large-size group showed more SN chromatin configurations, better cytoplasmic maturity and higher developmental potential than their counterparts in the medium-size group. It is concluded that BCB staining can be used as an efficient method for oocyte selection, but that the competence of the BCB＋ oocytes may vary with oocyte diameter, animal sexual maturity and gonadotropin stimulation. Taken together, the series of criteria described here would allow for better choices in selecting oocytes for better development.

Screening of Extraction Methods for Glycoproteins from Jellyfish (Rhopilema esculentum) Oral-Arms by High Performance Liquid Chromatography

In order to select an optimum extraction method for the target glycoprotein （TGP） from jellyfish （Rhopilema esculentum） oral-arms, a high performance liquid chromatography （HPLC）-assay for the determination of the TGP was developed Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear （r= 0.9984, y=4.5895x＋47.601） over concentrations ranging from 50 to 400mgL^-1. The mean extraction recovery was 97.84% （CV2.60%）. The fractions containing TGP were isolated from jellyfish （R esculentum） oral-arms by four extraction methods： 1） water extraction （WE）, 2） phosphate buffer solution （PBS） extraction （PE）, 3） ultrasound-assisted water extraction （UA-WE）, 4） ultrasound-assisted P/3S extraction （UA-PE）. The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL （7.8 mm×300 ram） column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.

The first report of treatment of liver abscess due to Candida albicans with intra-abscess and intravenous administration of liposomal amphotericin B (Amphotec)

Increasing reports on application and safety of liposomal amphotericin B (Amphotec) in the treatment of deep fungal infections have been described recently. This is the first report that a case of liver abscess due to Candida albicans was completely cured with intra-abscess and intravenous administration of liposomal amphotericin B without recurrence in three-year follow-up period.

EFFECTS OF SYSTEMIC FLUCONAZOLE THERAPY ON IN VITRO ADHESION OF CANDIDA ALBICANS TO BUCCAL EPITHELIAL CELLS AND CHANGES OF THE CELL SURFACE PROTEINS OF THE EPITHELIAL CELLS

This paper presented the effects of systemic fluconazole therapy via intravenous (IV) and oral (PO) administrations on the adhesion of Candida albicans (C. albicans) to the buccal epithelial cells (BEC) from five treated patients with three candidosis, one mucormycosis and one sporotrichosis and at the same time.an analysis of the cell surface proteins involving candidal adherent receptor in the BEC of the patients in the course of 7 days were exposed to ￣3H-leucine radiolabeled C. albicans for in vitro candidal adherent assay.and the BEC from first intake day and the last intake day of the patients were extracted by dithiothreitol (DTT)-iodoacetamide treatment for SDSPAGE. These results indicate that the systemic fluconazole therapy results in the inhibitory effect of candidal adhesion to BEC of treated patients to prevent them from oral candidosis for a prolonged time, which is based on the absent surface protein (35 KDa) of the BEC.

Using Magnetic Technique to Increase Efficiency of Organic Pollutants Biodegradation in Wastewater

The intensity of Magnetic field by 200, 300 and 400 gaos were selective to study their impacts on bacteria Bacillus, Pseudomonas and yeasts Candida dubliniensis, Candida glabrata, lssatchenkia orientalis and Rhodotorula mucilaginosa growth and to reduce organic pollutants in wastewater by COD, TOC, TN and TP in concentrations of 180, 75, 52 and 84 ppm in pH 7.6 and treatment periods of 2, 4, 6 and 18 h in batch system. Results showed that magnetic field 300 gaos had higher ability to increase bacterial and yeasts growth by 400-600% in 18 h and reduced COD, TOC, TN and TP by 88, 85, 90 and 98.5% in same period treatment. While, the intensity of magnetic field 200 and 400 gaos have no effect on microorganisms growth and reducing organic pollutants. This study is first record for showing and explaining the positive effective of magnetic field on microorganisms growth.

Intergenerational Transmission： Do Mothers Perceive Their Parenting Style and Parental Authority as Similar to Their Parents？

The research study examines whether intergenerational transmission of parenting style and parental authority exists, and whether this transmission is influenced by level of religiosity and national-ethnic origin. We refer to four parenting styles： authoritarian, authoritative, permissive and neglectful; the ＂parental authority＂ domain relates to parental authority exhibited toward children in all four styles. The study used a quantitative method, and 110 Jewish and Arab-Muslim mothers in Israel filled out self-report questionnaires on two generations. The findings are： intergenerational transmission of parenting style and parental authority exist; national-ethnic origin and level of religiosity do not influence intergenerational transmission of parenting style and parental authority.

Preferential Bivalent Formation in Tetraploid Male of Pacific Oyster Crassostrea gigas Thunberg

Artificially induced tetraploid Pacific oyster, Crassostrea gigas Thunberg, produces more aneuploid gametes than nor- mal diploid one, although they showed a comparable fecundity to diploidy. The meiotic chromosome configuration of 3 tetraploid and 1 tetraploid/triploid mosaic males were analyzed through direct chromosome observation. A majority of metaphase I spermato- cytes contained both bivalents and quadrivalents. The chromosome configuration of these males was characterized by preferential formation of bivalents to quadrivalents. Bivalents appeared in all spermatocytes and consisted of 86% of all chromosome aggregates. In comparison, quadrivalents occurred in 91% spermatocytes and consisted of only 12.6% of all chromosome aggregates. The mean bivalent frequency per spermatocyte varied between 14.4 and 17.2; while that of quadrivalents varied between 2.2 and 2.7. Most quadrivalents were tandemly chained （58%） or circled （39%）. The total number of chromosome aggregates per spermatocyte ranged from 13 to 20 with an average of 17.6; while 18 （16 bivalents and 2 quadrivalents） was the most frequent. Univalents and trivalents appeared in very low frequency. Aneuploid （hypotetraploid） spermatocytes were observed in a low frequency. The chromosome con- figuration of in the mosaic individual was similar to that of tetraploid individuals. The percentage of triploid spermatocytes （2%） of the mosaic individual was significantly lower （2＂2 =30, P〈 0.01） than that of triploid cells （46%） in its somatic tissue.

The Holy Bitch That Can Be a Witch in The Grass Harp

This paper comprises an analysis of the modernist American writer Truman Capote＇s novel The Grass Harp （1951） from a feminist perspective. While the novel treats the ostracizing of four people by the oppressive mindset of a patriarchal society, the female character Dolly Talbo who leads the banished group to live in a tree house becomes the embodiment of a Goddess image introduced by the New Age Spiritualities and Neopaganism. Creating a new culture for women as an alternative to the patriarchal system, in which concepts such as love, herbalism, and magic are sanctioned as sacred, and offering this culture as an opportunity to all human beings, Dolly Talbo can be perceived as a contemporary holy witch who becomes an occult and undermining threat to the patriarchal order.

Courtesy Language Research Based on Letter Style

Politeness principle, cooperation principle, social protocols, guidelines on the session, face preservation contract theory, as well as ＂politeness five criteria＂ of Chinese characteristics, and a series of principles and theories related to domestic and foreign research polite language provides a solid foundation, rich nutrients and scientific way of thinking for Chinese ＂polite language＂. This article combined with stylistic letter, takes ＂Fu Lei Letter＂ as the corpus to study online behavior polite language.

Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.

Assembly of long DNA sequences using a new synthetic Escherichia coli-yeast shuttle vector

Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and engineer complex artificial metabolic systems. DNA manipulation on a large genome-wide scale is an inevitable challenge, but a necessary tool for synthetic biology. To improve the methods used for the synthesis of long DNA fragments, here we constructed a novel shuttle vector named p GF(plasmid Genome Fast) for DNA assembly in vivo. The BAC plasmid p CC1 BAC, which can accommodate large DNA molecules, was chosen as the backbone. The sequence of the yeast artificial chromosome(YAC) regulatory element CEN6-ARS4 was synthesized and inserted into the plasmid to enable it to replicate in yeast. The selection sequence HIS3, obtained by polymerase chain reaction(PCR) from the plasmid p BS313, was inserted for screening. This new synthetic shuttle vector can mediate the transformation-associated recombination(TAR) assembly of large DNA fragments in yeast, and the assembled products can be transformed into Escherichia coli for further amplification. We also conducted in vivo DNA assembly using p GF and yeast homologous recombination and constructed a 31-kb long DNA sequence from the cyanophage PP genome. Our findings show that this novel shuttle vector would be a useful tool for efficient genome-scale DNA reconstruction.

A preliminary study of rare-metal mineralization in the Himalayan leucogranite belts, South Tibet

The Himalayan leucogranite occurs as two extensive(>1000 km) E-W trending belts on the Tibetan Plateau with the unique features. The leucogranite comprised biotite granite, two-mica/muscovite granite, tourmaline granite and garnet granite, which have been identified in previous studies, as well as albite granite and granitic pegmatite that were identified in this investigation. Fifteen leucogranite plutons were studied and 12 were found to contain rare-metal bearing minerals such as beryl(the representative of Be mineralization), columbite-group minerals, tapiolite, pyrochlore-microlite, fergusonite, Nb-Ta rutile(the representative of Nb-Ta mineralization), and cassiterite(the representative of Sn mineralization) mainly based on the field trip,microscope observation and microprobe analysis. The preliminary result shows that the Himalayan leucogranite is commonly related to the rare-metal mineralization and warrants future investigation. Further exploration and intensive research work is important in determining the rare-metal resource potential of this area.