选取2015、2018和2020年三次参加全国化学品毒性鉴定质量考核的18家机构作为研究对象,采用逼近理想解排序法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)和秩和比法二者模糊联合,对18家机构质量考核结...选取2015、2018和2020年三次参加全国化学品毒性鉴定质量考核的18家机构作为研究对象,采用逼近理想解排序法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)和秩和比法二者模糊联合,对18家机构质量考核结果进行综合分析和排序,为化学品毒性鉴定机构的管理工作提供理论依据。结果显示,所有考核项目中病理盲样阅片的平均成绩最低。TOPSIS法与秩和比法模糊联合分析优于使用其中一种方法单独分析,其所得结果排名前三名和后三名的机构,均为职业病防治研究院1家和疾控中心2家,分档排序后考核优秀机构有3家(J3,J1,Z5),合格机构2家(J7,Z4),其余机构为良好。提示,质量考核结果总体情况良好,病理学检验是考核薄弱项,化学品毒性鉴定机构应加强专业人才队伍建设。展开更多
[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain wa...[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain was isolated from the brain tissues of stillborn fetuses delivered by sows with suspected PRV infection and preliminarily identified by PCR. g E gene of the isolated PRV strain was amplified and sequenced for phylogenetic analysis. In addition, the pathogenicity of the isolated PRV strain to 6-week-old piglets was evaluated. [Result] A PRV strain was successfully isolated and named PRV N5 B strain, which could proliferate in Vero cells and TCID50 of the 15 thgeneration virus liquid reached 10^7.125/0.1 ml. Specific bands could be amplified by PCR. g E gene in the isolated PRV strain was 1 740 bp in length. A phylogenetic tree was constructed based on full-length g E sequences, which showed that PRV N5 B strain and PRV strains isolated since 2012 were clustered into the same independent category and shared 99.7%-100% homology of nucleotide sequences. Compared with related sequences published previously, there were insertions of three consecutive bases at two loci. Animal experiments showed that intranasal inoculation of 6-week-old piglets with 2 ml of PRV N5 B strain（10^6/0.1 ml） led to a mortality rate of 100%. [Conclusion] In this study,genetic variability of g E gene in PRV N5 B isolate and its pathogenicity to piglets were analyzed, which provided a theoretical basis for the development of new vaccines to prevent and control porcine pseudorabies.展开更多
文摘选取2015、2018和2020年三次参加全国化学品毒性鉴定质量考核的18家机构作为研究对象,采用逼近理想解排序法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)和秩和比法二者模糊联合,对18家机构质量考核结果进行综合分析和排序,为化学品毒性鉴定机构的管理工作提供理论依据。结果显示,所有考核项目中病理盲样阅片的平均成绩最低。TOPSIS法与秩和比法模糊联合分析优于使用其中一种方法单独分析,其所得结果排名前三名和后三名的机构,均为职业病防治研究院1家和疾控中心2家,分档排序后考核优秀机构有3家(J3,J1,Z5),合格机构2家(J7,Z4),其余机构为良好。提示,质量考核结果总体情况良好,病理学检验是考核薄弱项,化学品毒性鉴定机构应加强专业人才队伍建设。
基金Supported by Natural Science Foundation of Jiangsu Province(BK20131334)Fund for Independent Innovation of Agricultural Science and Technology in Jiangsu Province[CX(13)3069]~~
文摘[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain was isolated from the brain tissues of stillborn fetuses delivered by sows with suspected PRV infection and preliminarily identified by PCR. g E gene of the isolated PRV strain was amplified and sequenced for phylogenetic analysis. In addition, the pathogenicity of the isolated PRV strain to 6-week-old piglets was evaluated. [Result] A PRV strain was successfully isolated and named PRV N5 B strain, which could proliferate in Vero cells and TCID50 of the 15 thgeneration virus liquid reached 10^7.125/0.1 ml. Specific bands could be amplified by PCR. g E gene in the isolated PRV strain was 1 740 bp in length. A phylogenetic tree was constructed based on full-length g E sequences, which showed that PRV N5 B strain and PRV strains isolated since 2012 were clustered into the same independent category and shared 99.7%-100% homology of nucleotide sequences. Compared with related sequences published previously, there were insertions of three consecutive bases at two loci. Animal experiments showed that intranasal inoculation of 6-week-old piglets with 2 ml of PRV N5 B strain（10^6/0.1 ml） led to a mortality rate of 100%. [Conclusion] In this study,genetic variability of g E gene in PRV N5 B isolate and its pathogenicity to piglets were analyzed, which provided a theoretical basis for the development of new vaccines to prevent and control porcine pseudorabies.
