Comparison of methods of purification and identification of Verticillium dahliae toxins

The complexes excreted by VerticiUium dahliae are phytotoxins, which are responsible for most of the symptoms associated with Verticillium wilt disease. Verticillium dahliae toxins （VD-toxins） can be purified by different methods. In the present study, we reported a simpler, more effective method to purify VD-toxins. The supematant of V. dahliae culture was frozen, lyophilized and dialyzed by 1 kDa Dialysis Membranes （MWCO）. We also partially identified the characteristics of the purified VD-toxins. The results showed that the components of VD-toxins include glycoprotein within 35.8-83.2 kDa. The phytotoxic activity of VD-toxins was remained after VD-toxins were pretreated by high temperature, Concanavalin-A, and proteinase E, respectively. These data suggest that VD-toxins are heat-stable, and the protein fraction and glycosyl are both important contributors to the phytotoxic activity. VD-toxins purified effectively from the culture filtrates of V. dahliae may help in further understanding the mechanisms of interactions between V. dahliae and plants.

克劳德·伯尔纳——实验生理学的奠基人

克劳德·伯尔纳是 19世纪法国伟大的生理学家和一代哲人 ,他一生的实验研究几乎遍及生理学的各个领域。生理学家公认伯尔纳在胰腺的消化功能、肝脏的糖元生成功能、血管运动机制、箭毒和一氧化碳等毒物作用机制的研究方面都处于当时的领先地位。伯尔纳的研究为现代生理学和生物化学以及巴甫洛夫的工作奠定了基础 ,开创了实验生理学的先河。