HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity a...HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity and mortality worldwide. Currently,there are 30 drugs or combinations of drugs approved by FDA. Because of the side-effects,price and drug resistance,it is essential to discover new targets,to develop new technology and to find new anti-HIV drugs. This review summarizes the major targets and assays currently used in anti-HIV drug screening.展开更多
In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective...In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective of the present study was to evaluate the impact of leachate from a dump on surface water systems. Physical, chemical, and bacteriological characteristics and the volume of leachate were measured. The site of study is located in Zinaprcuaro, Michoac^m, Mexico, and was chosen due to the water supplies were in line with the dump, thus conforming a system to test for movement of toxins. Leachate and water from three ponds located at different distances to the dump were sampled during three months of a year to measure physicochemical and bacteriological parameters, according to the standard methods of the American Public Health Association; the concentration of heavy metals was measured by atomic absorption spectrometry. Most characteristics exceed the maximum permissible limits established for the Mexican legislation regarding wastewater discharges and drinking water. Likewise, analysis of variance showed statistically significant differences between concentrations of pollutants in relation to the distance from the dump of sampled sites and with season of sampling, thus confirming the contamination of water by dump leachate.展开更多
There is a growing number of case reports of hepatoxicity from the widely marketed weight-loss supplement Hydroxycut, which contains the botanical ingredient Garcinia cambogia . These case reports may substantially un...There is a growing number of case reports of hepatoxicity from the widely marketed weight-loss supplement Hydroxycut, which contains the botanical ingredient Garcinia cambogia . These case reports may substantially undercount the true magnitude of harm. Based on the past experience with harmful dietary supplements, US regulators should assume the more precautionary approach favored by Canada and Europe. Lacking effective adverse event surveillance for supplements, or the requirements to prove safety prior to coming to the market, case reports such as those summarized here assume added importance.展开更多
The three immediate-early proteins of HSV-1, ICP0, ICP22, and ICP27, have specific and pivotal functions in transcriptional activation and inhibition, multiple regulatory and control processes of viral genes. In this ...The three immediate-early proteins of HSV-1, ICP0, ICP22, and ICP27, have specific and pivotal functions in transcriptional activation and inhibition, multiple regulatory and control processes of viral genes. In this paper, the expression and localization of these three proteins were studied in neuroblastoma cells using biochemical assays, and their possible and potential interactive functions are discussed. The data show that the three proteins are localized in different structures, specifically in the PML-NB-associated structure, which is a specific nuclear structure composed of many protein molecules and bound tightly to the nuclear matrix in neuroblastoma cells. The results suggest that the activating and suppressive functions of ICPs are mostly dependent on their transcriptional and regulatory roles, including the PML-NB-associated structure.展开更多
文摘HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity and mortality worldwide. Currently,there are 30 drugs or combinations of drugs approved by FDA. Because of the side-effects,price and drug resistance,it is essential to discover new targets,to develop new technology and to find new anti-HIV drugs. This review summarizes the major targets and assays currently used in anti-HIV drug screening.
文摘In Mexico, hazardous and non-hazardous urban solid wastes are commonly disposed of in dumps. Inappropriate waste disposal makes solid waste disposal sites potential sources of pollution and health risks. The objective of the present study was to evaluate the impact of leachate from a dump on surface water systems. Physical, chemical, and bacteriological characteristics and the volume of leachate were measured. The site of study is located in Zinaprcuaro, Michoac^m, Mexico, and was chosen due to the water supplies were in line with the dump, thus conforming a system to test for movement of toxins. Leachate and water from three ponds located at different distances to the dump were sampled during three months of a year to measure physicochemical and bacteriological parameters, according to the standard methods of the American Public Health Association; the concentration of heavy metals was measured by atomic absorption spectrometry. Most characteristics exceed the maximum permissible limits established for the Mexican legislation regarding wastewater discharges and drinking water. Likewise, analysis of variance showed statistically significant differences between concentrations of pollutants in relation to the distance from the dump of sampled sites and with season of sampling, thus confirming the contamination of water by dump leachate.
文摘There is a growing number of case reports of hepatoxicity from the widely marketed weight-loss supplement Hydroxycut, which contains the botanical ingredient Garcinia cambogia . These case reports may substantially undercount the true magnitude of harm. Based on the past experience with harmful dietary supplements, US regulators should assume the more precautionary approach favored by Canada and Europe. Lacking effective adverse event surveillance for supplements, or the requirements to prove safety prior to coming to the market, case reports such as those summarized here assume added importance.
文摘The three immediate-early proteins of HSV-1, ICP0, ICP22, and ICP27, have specific and pivotal functions in transcriptional activation and inhibition, multiple regulatory and control processes of viral genes. In this paper, the expression and localization of these three proteins were studied in neuroblastoma cells using biochemical assays, and their possible and potential interactive functions are discussed. The data show that the three proteins are localized in different structures, specifically in the PML-NB-associated structure, which is a specific nuclear structure composed of many protein molecules and bound tightly to the nuclear matrix in neuroblastoma cells. The results suggest that the activating and suppressive functions of ICPs are mostly dependent on their transcriptional and regulatory roles, including the PML-NB-associated structure.
