目的：研究M受体亚型在正常成人前列腺组织中的分布状况。方法：取健康成人新鲜尸体前列腺标本17例，用免疫组织化学染色与荧光定量逆转录聚合酶链反应（Real Time RT-PCR）2种方法探讨M受体各亚型（M1-M3）在正常成人前列腺组织中的分...目的：研究M受体亚型在正常成人前列腺组织中的分布状况。方法：取健康成人新鲜尸体前列腺标本17例，用免疫组织化学染色与荧光定量逆转录聚合酶链反应（Real Time RT-PCR）2种方法探讨M受体各亚型（M1-M3）在正常成人前列腺组织中的分布特征。结果：2种方法分别从蛋白水平和基因水平证实M受体亚型（M1-M3）在正常成人前列腺组织中均有分布，其中以M2受体密度最高；各亚型主要分布在腺上皮周围，间质分布相对较少。结论：M受体亚型在正常成人前列腺组织中具有一定的分布规律，对发挥前列腺功能具有重要作用。展开更多
Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocam...Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocampus in the heroin sensitized rats. Methods Locomotor activity was measured every 10 min for 1 h after subcutaneous injection of heroin. FosB expression was assayed by immunohistochemistry, and the antisense oligonucleotides （AS-ONs） targeting M5 muscarinic receptor was transferred with the lipofectin. Results Microinjection of AS-ONs targeting M5 muscarinic receptor in the ventral tegmental area （VTA） blocked the expression of behavioral sensitization induced by heroin priming in rats. Meanwhile, the expression of FosB-positive neurons in either the NAc or the dentate gyrus （DG） of the hippocam- pus increased in heroin-induced locomotor sensitized rats. The enhancement of FosB-positive neurons in the NAc or DG could be inhibited by microinjection of M5 muscarinic receptor AS-ONs into the VTA before the heroin-induced locomotor sensitization was performed. In contrast, microinjection of M5 muscarinic receptor sense oligonucleotide （S-ONs） into the VTA did not block the expression of behavioral sensitization or the expression of FosB in the NAc or DG in the heroin sensitized rats. Conclusion Blocking M5 muscarinic receptor in the VTA inhibits the expression of heroin-induced locomotor sensitization, which is associated with the regulation of FosB expression in the NAc and hippocampus neurons. M5 muscarinic receptor may be a useful pharmacological target for the treatment of heroin addiction.展开更多
[Objective] The study aimed to extract the statistical features of complete tobacco necrosis virus genome and conduct clustering analysis.[Method] On the base sequence of complete tobacco necrosis virus genome,by usin...[Objective] The study aimed to extract the statistical features of complete tobacco necrosis virus genome and conduct clustering analysis.[Method] On the base sequence of complete tobacco necrosis virus genome,by using three base groups which was composed of each base and its subsequent two bases,a new sequence S was arranged;the probability of all 64 kinds of three base groups occurred on S was calculated,a 64-dimensional vector L was obtained;L-vector of each genome was compared,8 three base groups were obtained,their probabilities have significant differences.[Result] The emergence probability of 8 three base groups （CGT;CAA;TAC;TAA;AGG;ATG;ATA;AAG） has significant association with genetic variation of tobacco necrosis virus genome;according to genetic variation result,4 different sources tobacco necrosis virus complete genome formed 2 major categories.[Conclusion] The study will provide theoretical basis for the control of tobacco necrosis virus.展开更多
文摘目的：研究M受体亚型在正常成人前列腺组织中的分布状况。方法：取健康成人新鲜尸体前列腺标本17例，用免疫组织化学染色与荧光定量逆转录聚合酶链反应（Real Time RT-PCR）2种方法探讨M受体各亚型（M1-M3）在正常成人前列腺组织中的分布特征。结果：2种方法分别从蛋白水平和基因水平证实M受体亚型（M1-M3）在正常成人前列腺组织中均有分布，其中以M2受体密度最高；各亚型主要分布在腺上皮周围，间质分布相对较少。结论：M受体亚型在正常成人前列腺组织中具有一定的分布规律，对发挥前列腺功能具有重要作用。
基金This work was supported by the National Nature Science Foundation of China （No.30470554）the National Basic Research Development Program of China（No.2003CB515404）.
文摘Objective To investigate the effect of M5 muscarinic receptor subtype on the locomotor sensitization induced by heroin priming, and it＇s effect on the FosB expression in the nucleus accumbens （NAc） and the hippocampus in the heroin sensitized rats. Methods Locomotor activity was measured every 10 min for 1 h after subcutaneous injection of heroin. FosB expression was assayed by immunohistochemistry, and the antisense oligonucleotides （AS-ONs） targeting M5 muscarinic receptor was transferred with the lipofectin. Results Microinjection of AS-ONs targeting M5 muscarinic receptor in the ventral tegmental area （VTA） blocked the expression of behavioral sensitization induced by heroin priming in rats. Meanwhile, the expression of FosB-positive neurons in either the NAc or the dentate gyrus （DG） of the hippocam- pus increased in heroin-induced locomotor sensitized rats. The enhancement of FosB-positive neurons in the NAc or DG could be inhibited by microinjection of M5 muscarinic receptor AS-ONs into the VTA before the heroin-induced locomotor sensitization was performed. In contrast, microinjection of M5 muscarinic receptor sense oligonucleotide （S-ONs） into the VTA did not block the expression of behavioral sensitization or the expression of FosB in the NAc or DG in the heroin sensitized rats. Conclusion Blocking M5 muscarinic receptor in the VTA inhibits the expression of heroin-induced locomotor sensitization, which is associated with the regulation of FosB expression in the NAc and hippocampus neurons. M5 muscarinic receptor may be a useful pharmacological target for the treatment of heroin addiction.
基金Supported by"Eleventh Five-year" Education Science Development Plan of Hubei Province (2006B131)~~
文摘[Objective] The study aimed to extract the statistical features of complete tobacco necrosis virus genome and conduct clustering analysis.[Method] On the base sequence of complete tobacco necrosis virus genome,by using three base groups which was composed of each base and its subsequent two bases,a new sequence S was arranged;the probability of all 64 kinds of three base groups occurred on S was calculated,a 64-dimensional vector L was obtained;L-vector of each genome was compared,8 three base groups were obtained,their probabilities have significant differences.[Result] The emergence probability of 8 three base groups （CGT;CAA;TAC;TAA;AGG;ATG;ATA;AAG） has significant association with genetic variation of tobacco necrosis virus genome;according to genetic variation result,4 different sources tobacco necrosis virus complete genome formed 2 major categories.[Conclusion] The study will provide theoretical basis for the control of tobacco necrosis virus.
