宫颈高危型人乳头瘤病毒感染与宫颈病变相关性研究

目的研究宫颈高危型人乳头瘤病毒(high-risk human papillomavirus,HR-HPV),以及病毒载量与宫颈病变患者的相关性,为宫颈病变患者诊断及防治提供参考。方法收集我院2016年10月至2017年8月妇科经阴道镜活检确诊的宫颈上皮内瘤样病变(CIN)患者共90例(且均行HPV分型及病毒载量检测),其中CINⅠ级30例、CINⅡ级30例、CINⅢ级30例。结果高危型HPV的阳性率达81.11%(73/90),其中感染HPV16和(或)HPV18患者所占比为35.56%(32/90),感染其他高危型HPV患者占比为45.56%(41/90),感染低危型HPV患者占比为11.11%(10/90),未感染HPV患者占比例为7.78%(7/90);HR-HPV病毒载量和宫颈病变严重情况为正相关性,CINⅠ级HR-HPV病毒载量<100 RLU/CO,CINⅡ-CINⅢ级HR-HPV病毒载量为≥100 RLU/CO。结论 HR-HPV病毒载量可作为诊断CINⅡ级以上病变的指标,HR-HPV病毒载量为100 RLU/CO是导致宫颈高度病变的阳性分界点。

Reduction of virus burden-induced splenectomy in patients with liver cirrhosis related to hepatitis C virus infection

AIM： To examine the hepatitis C virus （HCV） levels and immunological markers in cirrhotic patients after splenectomy. METHODS： HCV RNA titers as well as cellular and humoral immune markers were determined in 20 cirrhotic patients after splenectomy and in 32 cirrhotic controls with an intact spleen. RESULTS： Serum HCV RNA titers were lower in the splenectomized patients than in the controls （186 ± 225 × 10^3 copies/mL vs 541 ± 417×10^3 copies/mL, P〈0.01）. HCV RNA was judged to have been spontaneously eradicated in 4 splenectomized patients, but in none of the controls. Natural killer cell activity was higher in the splenectomized patients than in the controls （41.2 ± 19.3% vs 24.7 ± 15.3%, P〈 0.01）, and natural killer cell activity was negatively correlated to HCV RNA titers in the splenectomized patients except in those with serotype 2-related infection. The CEH/CD8 ratio was significantly lower in the splenectomized patients than in the controls. CONCLUSION： The findings suggest that splenectomy may diminish virus burden in cirrhotic patients with HCV infection at least in part, through augmentation of natural killer cell activity.

Effects of Duck Tembusu Virus on Serum Biochemical Indexes, Cytokines and Viral Replication of Ducklings

In order to understand the pathogenicity of duck Tembusu virus （DTMUV）, it was injected into muscle of 5-d-old Cherry Valley ducklings according to the dosage of 1×104 EID50. Then, the biochemical indexes of duckling serum samples were determined by kits, and the changes in detoxification, tissue viral load and cytokines were detected by using fluorescence quantitative PCR. The results showed that DTMUV had serious damage to the liver, kidney, heart and muscle of ducklings; DTMUV could proliferate in the liver, spleen, lung and brain; the virus levels in the liver and brain reached the peaks on day 5 after the inoculation and those in the lung and spleen reached the peaks on day 9; the virus content was highest in the brain, liver and spleen; and DTMUV induced the overexpression of IFN-γ, IFN-α, IL-6, IFN-β, IL-1β, TLR-7,IL-2, major histocompatibility complex type I （MHC-I） andmajor histocompatibility complex type II （MHC-II） in the spleen on day 1 and the overexpression of IL-6 and IL-2 in the brain on days 1, 2 and 3.

DNA-guided hepatitis B treatment:Viral load is insufficient with few exceptions

In DNA-guided hepatitis B treatment, viral load is insufficient, and requires other viral markers for treatment of hepatitis B patients as in patients with acute exacerbation of chronic hepatitis B, end-stage renal disease on dialysis, human immunodeficiency virus co-infected patients. There are exceptions to this rule： a residual level hepatitis B virus （HBV） DNA at 24 wk predicts beneficial outcome and reduced resistance at i year. The genotypic viral resistance to antiviral agents and occult HBV infection can be determined by HBV-DNA levels.