AIM：To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cis- platin on two cancer cell lines. METHODS： Colorectal carcinoma （HT29） and human hepatocellular carcin...AIM：To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cis- platin on two cancer cell lines. METHODS： Colorectal carcinoma （HT29） and human hepatocellular carcinoma （HepG2） cells were treated with spray-dried extracts of Phyllanthus niruri （SDEPN） either alone or in combination with cisplatin at differ- ent concentrations （0.5 mg/mL and 1 mg/mL） for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with prop- idium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histo- grams. Significant differences between groups were determined using analysis of variance and Bonferroni＇s test, as indicated. A value of P 〈 0.05 was considered to be statistically significant. RESULTS： SDEPN had significantly different cyto- toxic effects on HT29 （2.81 4- 0.11 vs 3.51 4- 1.13, P 〉 0.05） and HepG2 （5.07± 0.3 vs 15.9 ± 1.04, P 〈 0.001） cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 （1.91 ± 0.57 vs 4.53± 1.22, P 〉 0.05） and HepG2 （14.56 ± 1.6 vs 35.67 ± 3.94, P 〈 0.001） cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells （HepG2 cells for 4 h： 10.78 ± 1.58 vs 53.89 ± 1.53, P 〈 0.001; 24 h： 8.9 ± 1.43 vs 62.78 ± 1.87, P 〈 0.001 and HT29 cells for 4 h： 9.52 ±0.913 vs 49.86 ± 2.89, P 〈 0.001; 24 h： 11.78 ± 1.05 vs 53.34 ± 2.65, P 〈 0.001）. In HT29 cells, pretreat- ment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed （12.78 ± 1.01 vs 93.76 ± 1.6, P 〈 0.001）. HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin （12.87 ± 2.78 vs 78.8 ± 3.02, P 〈 0.001）. CONCLUSION： SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.展开更多
Bench-scale soil column experiments were carried out to evaluate the effectiveness of Cr（VI） bioremediation process in soils by using indigenous bacteria with the addition of bacteria nutrient media. Effects of part...Bench-scale soil column experiments were carried out to evaluate the effectiveness of Cr（VI） bioremediation process in soils by using indigenous bacteria with the addition of bacteria nutrient media. Effects of particle size, spray intensity, initial Cr（VI） concentration, circulation mode and soil depth on Cr（VI） remediation were studied. Results show that soils after 6 d remediation with spray intensity controlled in the range of 29.6-59.2 mL/min could well fulfill the requirement of concrete aggregate and roadbed material usage, for the leaching toxicity concentration of the Cr（VI） in treated soils under the chosen condition is far less than 5 mg/L The leaching toxicity and fractions of both hexavalent chromium and trivalent chromium from remediated soils were determined and compared with that of untreated soil. The results show that water soluble Cr（VI） declines from 1520.54 mg/kg to 0.68 mg/kg, exchangeable Cr（VI） decreases from 34.83 mg/kg to 0.01 mg/kg and carbonates-bonded Cr（V1） falls from 13.55 mg/kg to 0.68 mg/kg. Meanwhile, a corresponding increase in carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（III） and organic matter-bonded Cr（III） are found. It reveals that indigenous bacteria can leach out water soluble Cr（VI）, exchangeable Cr（VI） and carbonates-bonded Cr（VI） from contaminated soil followed by converting into carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（IlI）, organic matter-bonded Cr（III） and residual Cr（III）.展开更多
基金Supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq (470179/2009-0) for financial support and Postgraduate Program in Pharmaceutical Sciences,Federal University of Rio Grande do Norte
文摘AIM：To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cis- platin on two cancer cell lines. METHODS： Colorectal carcinoma （HT29） and human hepatocellular carcinoma （HepG2） cells were treated with spray-dried extracts of Phyllanthus niruri （SDEPN） either alone or in combination with cisplatin at differ- ent concentrations （0.5 mg/mL and 1 mg/mL） for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with prop- idium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histo- grams. Significant differences between groups were determined using analysis of variance and Bonferroni＇s test, as indicated. A value of P 〈 0.05 was considered to be statistically significant. RESULTS： SDEPN had significantly different cyto- toxic effects on HT29 （2.81 4- 0.11 vs 3.51 4- 1.13, P 〉 0.05） and HepG2 （5.07± 0.3 vs 15.9 ± 1.04, P 〈 0.001） cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 （1.91 ± 0.57 vs 4.53± 1.22, P 〉 0.05） and HepG2 （14.56 ± 1.6 vs 35.67 ± 3.94, P 〈 0.001） cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells （HepG2 cells for 4 h： 10.78 ± 1.58 vs 53.89 ± 1.53, P 〈 0.001; 24 h： 8.9 ± 1.43 vs 62.78 ± 1.87, P 〈 0.001 and HT29 cells for 4 h： 9.52 ±0.913 vs 49.86 ± 2.89, P 〈 0.001; 24 h： 11.78 ± 1.05 vs 53.34 ± 2.65, P 〈 0.001）. In HT29 cells, pretreat- ment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed （12.78 ± 1.01 vs 93.76 ± 1.6, P 〈 0.001）. HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin （12.87 ± 2.78 vs 78.8 ± 3.02, P 〈 0.001）. CONCLUSION： SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.
基金Project(50925417) supported by the National Funds for Distinguished Young Scientist, ChinaProject(50830301) supported by the Key Program of National Natural Science Foundation of ChinaProject(51074191) supported by the National Natural Science Foundation of China
文摘Bench-scale soil column experiments were carried out to evaluate the effectiveness of Cr（VI） bioremediation process in soils by using indigenous bacteria with the addition of bacteria nutrient media. Effects of particle size, spray intensity, initial Cr（VI） concentration, circulation mode and soil depth on Cr（VI） remediation were studied. Results show that soils after 6 d remediation with spray intensity controlled in the range of 29.6-59.2 mL/min could well fulfill the requirement of concrete aggregate and roadbed material usage, for the leaching toxicity concentration of the Cr（VI） in treated soils under the chosen condition is far less than 5 mg/L The leaching toxicity and fractions of both hexavalent chromium and trivalent chromium from remediated soils were determined and compared with that of untreated soil. The results show that water soluble Cr（VI） declines from 1520.54 mg/kg to 0.68 mg/kg, exchangeable Cr（VI） decreases from 34.83 mg/kg to 0.01 mg/kg and carbonates-bonded Cr（V1） falls from 13.55 mg/kg to 0.68 mg/kg. Meanwhile, a corresponding increase in carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（III） and organic matter-bonded Cr（III） are found. It reveals that indigenous bacteria can leach out water soluble Cr（VI）, exchangeable Cr（VI） and carbonates-bonded Cr（VI） from contaminated soil followed by converting into carbonate-bonded Cr（III）, Fe and Mn oxides-bonded Cr（IlI）, organic matter-bonded Cr（III） and residual Cr（III）.
