槲皮素对人毛囊乳头细胞氧化损伤的干预作用

雄激素性脱发(androgeneticalopecia,AGA)是一种由激素、遗传、环境及年龄因素相互复杂作用的多基因疾病,是最常见的进行性脱发类型[1]。根据国家卫健委发布的数据显示,我国有超2.5亿人正饱受脱发的困扰,且这种现象不断年轻化。AGA的进行性提示着该病症不会自行缓解,必须要进行干预。

Wnt信号通路在毛囊乳头细胞诱导毛囊形成及生长过程中作用的研究新进展

大面积脱发治疗是目前面临的难题,毛囊的体外培养与移植是很有潜力的治疗方案,但临床上极少有成功的案例。毛囊形成、生长调控机制复杂,体外培养难以模拟,在当前研究中毛囊乳头细胞诱导毛囊形成是体外毛囊培养的主要方法。Wnt信号通路在毛囊乳头细胞诱导毛囊形成和周期再生的调控中起核心作用。该文就Wnt信号通路在毛囊乳头细胞诱导毛囊形成和周期生长过程中作用的最新研究,以及近几年体外培养毛囊的新方法作一综述,希望联合不同培养方法和调控因素的体外培养能为临床大面积脱发治疗提供新的思路和依据。

胎鼠表皮干细胞的分离培养及毛囊再生

AIM: To culture fetal murine epidermal stem cells and follicular papilla cells in vitro and to investigate the regeneration of epidermis and follicle on animal models. METHODS: The fetal murine epidermal stem cells (ESCs) were isolated by adhering to collagen type IV and cultured in conditional medium in vitro. Then, the ESCs were identified by intergrin β 1 and keratin15 antibodies, and the cell cycle profile and colony forming efficiency (CFE) were also studied. Meanwhile the follicular papilla cells were cultured from single follicle and grown in fibrin gel. Finally, the ESCs and papilla cells were transplanted on the full skin wound of BALB/C nude mouse together and the regeneration of epidermis and follicle would be investigated in the following 8～10 weeks. RESULTS: The ESCs isolated by collagen type IV were intergrin β 1 and kerain15 staining positive. The cell cycle profile analysis showed that 94.9% of the ESCs were at G1 stage and only 3.5% at S stage. As the control group, 74.1% of keratinocytes were at G1 stage and 17.5% at S stage. The CFE of ESCs was 15.3% and that of keratinocytes was 6.7%. After covered by ESCs and papilla cells for 8～10 weeks, the regeneration of epidermis and follicle were observed on the back of the mouse. CONCLUSION: The epidermal stem cells and follicular papilla cells could be isolated and cultured in vitro, and the epidermal stem cells could take part in forming the new follicle and epidermis structure induced by follicular papilla cells.

中药成分联合米诺地尔抗雄激素性脱发的作用研究

目的研究中药成分与米诺地尔联用抗雄激素性脱发的作用。方法将莽草酸、黄芩苷和雪松醇3种中药成分分别与米诺地尔联用,采用人毛囊乳头细胞(human dermal papilla cells,hDPC)细胞增殖试验验证中药成分协同米诺地尔提高细胞活力的作用,并确定最佳联用比例,利用睾酮干扰的C57BL/6小鼠模拟雄激素性脱发模型评价药物联用组合促进毛发再生的效果,5α-还原酶抑制试验探究雪松醇对抗雄激素性脱发的起效机制。结果细胞增殖试验表明,莽草酸、黄芩苷和雪松醇3种中药成分在5μmol·L^(-1)浓度下均能发挥促进细胞增殖的作用,且3种中药成分与米诺地尔联用都能协同提高细胞活力;雪松醇与米诺地尔1∶1摩尔比联用、黄芩苷与米诺地尔1∶1摩尔比联用的细胞存活率最高。体内药效学试验表明,雪松醇与米诺地尔联合给药组的同期毛发覆盖率、再生毛发长度药效评价指标都显著高于其他组别。5α-还原酶抑制试验显示雪松醇对5α-还原酶的抑制率>(69.60±0.89)%,雪松醇-米诺地尔联用对5α-还原酶的抑制作用显著高于米诺地尔。结论莽草酸、黄芩苷和雪松醇3种中药成分与米诺地尔联用均能协同促进人毛囊乳头细胞增殖。雪松醇-米诺地尔1∶1联用在提高细胞活力、促进毛囊分化、诱导毛囊周期转变、促进毛发再生方面都显示出良好的协同增效作用,可以作为抗雄激素性脱发的最佳联合给药方案。抑制5α-还原酶活性是雪松醇的主要起效机制之一。