A full set of disease resistance(R) candidate genes encoding nucleotide-binding sites(NBS) in a complete genome of Populus trichocarpa was identified and characterized by structural diversities,physical positions,phyl...A full set of disease resistance(R) candidate genes encoding nucleotide-binding sites(NBS) in a complete genome of Populus trichocarpa was identified and characterized by structural diversities,physical positions,phylogenetic relationships.Based on structures of N-terminal motif and leucine-rich repeat domains motif,we found 381 NBS-coding sequences with 122 non-regular NBS genes and 259 regular NBS genes that were further classified into 13 types such as TNL,CNL,NL,XNL,TN and other minor types.Meanwhile 81.9% of the NBS genes were distributed in cluster,and 81.8% of the cluster genes had duplicates.The results showed that there were many duplicate phenomenon occurred in the evolution of disease resistance genes of P.trichocarpa.After analysis of NBS standard phylogenetic tree in the genome of P.trichocarpa,the structure of tree exhibited a star topology,and the regular NBS genes were classified into 68 groups by less than 30% amino acid sequence diversity in each domain.展开更多
以毛果杨(Populus trichocarpa)幼嫩叶片为材料,进行原生质体分离,结果表明,酶解液(20 mL)的组成为1.5%纤维素酶R10+0.4%离析酶+2 mL 0.2 mol/L MES+10 mL 0.8 mol/L甘露醇+0.2 mL 2 mol/L KCl,所得原生质体为2×10^5个细胞/mL。在...以毛果杨(Populus trichocarpa)幼嫩叶片为材料,进行原生质体分离,结果表明,酶解液(20 mL)的组成为1.5%纤维素酶R10+0.4%离析酶+2 mL 0.2 mol/L MES+10 mL 0.8 mol/L甘露醇+0.2 mL 2 mol/L KCl,所得原生质体为2×10^5个细胞/mL。在此基础上,对白桦中一个FLA(fasciclin-like arabinogalactan-protein)基因BpFLA20进行了亚细胞定位研究。系统进化分析显示,BpFLA20蛋白在序列上与次生壁合成相关的AtFLA11和AtFLA12蛋白相近。以白桦的根、茎、叶cDNA为模版,进行了荧光定量PCR实验,结果表明该基因在根中的表达量最高,在叶中的表达量最低。以本研究分离的毛果杨原生质体为受体,利用PEG介导的原生质体瞬时转化法将35S::FLA20-GFP融合表达载体转化到毛果杨原生质体中进行亚细胞定位研究,结果显示,BpFLA20蛋白定位于细胞膜。展开更多
文摘A full set of disease resistance(R) candidate genes encoding nucleotide-binding sites(NBS) in a complete genome of Populus trichocarpa was identified and characterized by structural diversities,physical positions,phylogenetic relationships.Based on structures of N-terminal motif and leucine-rich repeat domains motif,we found 381 NBS-coding sequences with 122 non-regular NBS genes and 259 regular NBS genes that were further classified into 13 types such as TNL,CNL,NL,XNL,TN and other minor types.Meanwhile 81.9% of the NBS genes were distributed in cluster,and 81.8% of the cluster genes had duplicates.The results showed that there were many duplicate phenomenon occurred in the evolution of disease resistance genes of P.trichocarpa.After analysis of NBS standard phylogenetic tree in the genome of P.trichocarpa,the structure of tree exhibited a star topology,and the regular NBS genes were classified into 68 groups by less than 30% amino acid sequence diversity in each domain.
文摘以毛果杨(Populus trichocarpa)幼嫩叶片为材料,进行原生质体分离,结果表明,酶解液(20 mL)的组成为1.5%纤维素酶R10+0.4%离析酶+2 mL 0.2 mol/L MES+10 mL 0.8 mol/L甘露醇+0.2 mL 2 mol/L KCl,所得原生质体为2×10^5个细胞/mL。在此基础上,对白桦中一个FLA(fasciclin-like arabinogalactan-protein)基因BpFLA20进行了亚细胞定位研究。系统进化分析显示,BpFLA20蛋白在序列上与次生壁合成相关的AtFLA11和AtFLA12蛋白相近。以白桦的根、茎、叶cDNA为模版,进行了荧光定量PCR实验,结果表明该基因在根中的表达量最高,在叶中的表达量最低。以本研究分离的毛果杨原生质体为受体,利用PEG介导的原生质体瞬时转化法将35S::FLA20-GFP融合表达载体转化到毛果杨原生质体中进行亚细胞定位研究,结果显示,BpFLA20蛋白定位于细胞膜。
