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砷污染土壤中毛球菌的分离及其砷还原特性 被引量:1
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作者 刘志进 杨小琴 段晋明 《微生物学杂志》 CAS CSCD 2018年第4期56-61,共6页
生物异化还原作用是地球化学循环中的重要部分,其对环境污染物具有良好的治理作用。从湖南省石门县的土壤中分离出1株厌氧砷还原菌,通过16S rRNA分析为毛球菌属,命名为Trichococcus sp.CC328。菌落圆形,无色透明,短杆状革兰阴性菌,最适... 生物异化还原作用是地球化学循环中的重要部分,其对环境污染物具有良好的治理作用。从湖南省石门县的土壤中分离出1株厌氧砷还原菌,通过16S rRNA分析为毛球菌属,命名为Trichococcus sp.CC328。菌落圆形,无色透明,短杆状革兰阴性菌,最适生长条件为p H 9,Na Cl浓度12-16 g/L。当As(V)的初始浓度为63mg/L时,24 h时细胞数目最多(18×108个/m L),48 h时砷还原率为83%;当Fe(III)的初始浓度为30 mg/L,72 h时还原率为37%。本研究是首次发现具有砷和铁还原能力的毛球菌,为土壤中砷的生物转化提供参考。 展开更多
关键词 砷污染 分离 厌氧 毛球菌 砷还原
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侧柏内生真菌Chaetomium globosum ZH-32抗菌活性成分研究 被引量:10
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作者 张文豪 国政 +1 位作者 魏少鹏 姬志勤 《农药学学报》 CAS CSCD 北大核心 2014年第5期605-609,共5页
采用生物活性追踪法,从侧柏内生真菌Chaetomium globosum ZH-32发酵液中分离得到1个具有抑菌活性的化合物——WH-01,经紫外光谱、核磁共振及质谱等鉴定为球毛壳菌素A。抑菌活性测定结果表明:WH-01对枯草芽孢杆菌Bacillus subtilis、蜡... 采用生物活性追踪法,从侧柏内生真菌Chaetomium globosum ZH-32发酵液中分离得到1个具有抑菌活性的化合物——WH-01,经紫外光谱、核磁共振及质谱等鉴定为球毛壳菌素A。抑菌活性测定结果表明:WH-01对枯草芽孢杆菌Bacillus subtilis、蜡状芽孢杆菌Bacillus cereus、金黄色葡萄球菌Staphylococcus aureus和大肠埃希氏菌Escherichia coli有强烈的抑制作用,其最低抑制浓度(MIC)分别为3.13、3.13、1.56和3.13μg/mL;对番茄灰霉病菌Botrytis cinerea和油菜菌核病菌Sclerotinia sclerotiorum菌丝生长的抑制中浓度(EC50)分别为5.36和12.19μg/mL。 展开更多
关键词 内生真菌ZH-32 抑菌活性 侧柏 ENDOPHYTIC FUNGUS ZH-32
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Synthesis of a New Series of Chalcone Derivatives and Their Antifungal Activities 被引量:7
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作者 李正香 李安良 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第4期245-248,共4页
To study the antifungal effect of chalcone derivatives. Methods Sixteenchalcone derivatives were synthesized and confirmed by ~1H NMR and IR spectra, and tested forantifungal activity against four common pathogenic fu... To study the antifungal effect of chalcone derivatives. Methods Sixteenchalcone derivatives were synthesized and confirmed by ~1H NMR and IR spectra, and tested forantifungal activity against four common pathogenic fungi. Their structure-activity relationship isdiscussed. Results Among 16 title compounds, there were 5 new compounds, which have not beenreported before. The preliminary antifungal test showed that all title compounds exhibitedantifungal activities to a certain extent. The activity of compound 8 against Trichophyton rubrumhad a potency equal to that of fluconazole, with a MIC of 4 μg·mL^(-1) . Conclusion Sixteenchalcones were prepared and their antifungal activities against four common pathogenic fungi invitro were examined. Some of them exhibited antifungal activities to a certain extent. 展开更多
关键词 CHALCONE antifungal agent in vitro
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Effect of Chaetomium globosum ND35 on Plant Growth and Preliminary Study of Its Biocontrol Efficacy
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作者 余新燕 孟庆果 +3 位作者 任思栋 刘振叶 王茂云 高克祥 《Agricultural Science & Technology》 CAS 2009年第5期126-129,共4页
[Objective] The aim was to study effect of Chaetomium globosum ND35 on plant growth and preliminary study of its biocontrol efficacy, and provide basis for popularization and application of this strain.[ Method] With ... [Objective] The aim was to study effect of Chaetomium globosum ND35 on plant growth and preliminary study of its biocontrol efficacy, and provide basis for popularization and application of this strain.[ Method] With endophytic fungus C. globosum ND35 as a tested strain, effect of C. globosumND35 on plant growth and its biocontrol on five plant diseases were investigated in the greenhouse and field,[Result]The results showed that ND35 promoted growth of lateral root and diameter of breast height of poplar. ND35 can induce poplar to resist Poplar Valsa Canker caused by Valsa sordida and Poplar Rust caused by Melampsora puplicola. ND35 was also able to induce tomato and bean to resist Botrytis cinera. Biocontrol of Bean Stem Rot Rhizoctonia by ND35 was effective as well. [Conclusion] Induced systemic resistance by endophytic C. globosum ND35 plays an important role in biocontrol of plant diseases. 展开更多
关键词 Endophytic fungus Chaetomium globosum ND35 Induced systemic resistance BIOCONTROL
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Cloning and expression of Hsp22.4 gene from Chaetomium globosum
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作者 LIU Zhi-hua YANG Qian NIE Yi-huang 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第3期259-262,共4页
A study was conducted on the molecular mechanism of small heat shock proteins (sHSPs) in Chaetomium globosum. Heat shock protein 22.4 (Hsp22.4) from C. globosum was cloned and expressed in Escherichia coli. BlastX... A study was conducted on the molecular mechanism of small heat shock proteins (sHSPs) in Chaetomium globosum. Heat shock protein 22.4 (Hsp22.4) from C. globosum was cloned and expressed in Escherichia coli. BlastX analysis revealed that the Hsp22.4 gene from C. globosum shared the highest identity in amino acid sequence with a Hsp gene from Neurospora crassa, and the identity between them was 65%. The C. globosum Hsp22.4 gene was inserted into the expressive vector of pGEX-4T-2 and the recombinant plasmid named pGEX-HSE E. coli BL21 transformed with pGEX-HSP plasmid was induced by IPTG, and the expressed proteins were analyzed with SDS-PAGE. A 50 kD protein was specially expressed in E. coli BL21, and the result was consistent with expectation, and showed that the Hsp22.4 gene had been expressed in E. coli. Our study has made a foundation for further studying the function ofsHSPs protein. 展开更多
关键词 Chaetomium globosum Heat shock proteins (HSPs) Gene cloning and Expression
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A PKS gene, pks-1, is involved in chaetoglobosin biosynthesis, pigmentation and sporulation in Chaetomium globosum 被引量:7
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作者 HU Yang HAO XiaoRan LOU Jing ZHANG Ping PAN Jiao ZHU XuDong 《Science China(Life Sciences)》 SCIE CAS 2012年第12期1100-1108,共9页
Chaetomium globosum is one of the most common fungi in nature. It is best known for producing chaetoglobosins; however, the molecular basis of chaetoglobosin biosynthesis is poorly understood in this fungus. In this s... Chaetomium globosum is one of the most common fungi in nature. It is best known for producing chaetoglobosins; however, the molecular basis of chaetoglobosin biosynthesis is poorly understood in this fungus. In this study, we utilized RNA inter- ference (RNAi) to characterize a polyketide synthase gene, pks-1, in C. globosum that is involved in the production of chaeto- globosin A. When pks-1 was knocked down by RNAi, the production of chaetoglobosin A dramatically decreased. Knock-down mutants also displayed a pigment-deficient phenotype. These results suggest that the two polyketides, melanin and chaetoglobosin, are likely to share common biosynthetic steps. Most importantly, we found that pks-I also plays a critical role in sporulation. The silenced mutants ofpks-1 lost the ability to produce spores. We propose that polyketides may modulate cellular development via an unidentified action. We also suggest that C. globosum pks-1 is unique because of its triple role in melanin formation, chaetoglobosin biosynthesis and sporulation. This work may shed light on chaetoglobosin biosynthesis and indicates a relationship between secondary metabolism and fungal morphogenesis. 展开更多
关键词 polyketide synthase (PKS) MELANIN chaetoglobosin A Chaetomium globosum
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