In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(P...In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab 2-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.展开更多
A novel aerodynamic force capillary microdrop-injection-chemiluminescence(AFCM-CL) system for the determination of ethamsylate was proposed in this paper. In this system the ethamsylate microdrop formed at the end of ...A novel aerodynamic force capillary microdrop-injection-chemiluminescence(AFCM-CL) system for the determination of ethamsylate was proposed in this paper. In this system the ethamsylate microdrop formed at the end of the capillary droped into the reactor cell by the force of the gravity and the high press gas automatically. Then the ethamsylate was directly oxidized by dissolved oxygen in the presence of hexacyanoferrate(Ⅲ) to generate a CL signal in sodium hydroxide medium. The phenomena of sample zone dilution and spreading disappeared as the capillary was used as the sample channel and gas pressure was used as driving force without the carrier stream. Therefore, a high sensitivity was obtained compared with the FI-CL method. The analysis sample volume is only about 8 μL and the sample throughput is 180 h -1. The system shows that the ethamsylate is detected linearly in the mass concentration range from 5×10 -10—1×10 -6 g/mL. The detection limit is 1.4×10 -10 g/mL(1.1 pg). The relative standard deviation is 3.1% at 2.5×10 -8 g/mL(n=7).展开更多
文摘In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab 2-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.
文摘A novel aerodynamic force capillary microdrop-injection-chemiluminescence(AFCM-CL) system for the determination of ethamsylate was proposed in this paper. In this system the ethamsylate microdrop formed at the end of the capillary droped into the reactor cell by the force of the gravity and the high press gas automatically. Then the ethamsylate was directly oxidized by dissolved oxygen in the presence of hexacyanoferrate(Ⅲ) to generate a CL signal in sodium hydroxide medium. The phenomena of sample zone dilution and spreading disappeared as the capillary was used as the sample channel and gas pressure was used as driving force without the carrier stream. Therefore, a high sensitivity was obtained compared with the FI-CL method. The analysis sample volume is only about 8 μL and the sample throughput is 180 h -1. The system shows that the ethamsylate is detected linearly in the mass concentration range from 5×10 -10—1×10 -6 g/mL. The detection limit is 1.4×10 -10 g/mL(1.1 pg). The relative standard deviation is 3.1% at 2.5×10 -8 g/mL(n=7).
