AIM： To elucidate the role of neuropilin-1 （Nrp-1） and semaphorin 3A （Sema3A） in sinusoidal remodeling dur- ing liver regeneration in rats. METHODS： Male Wistar/ST rats at 7 wk of age, weigh- ing about 200 g, we...AIM： To elucidate the role of neuropilin-1 （Nrp-1） and semaphorin 3A （Sema3A） in sinusoidal remodeling dur- ing liver regeneration in rats. METHODS： Male Wistar/ST rats at 7 wk of age, weigh- ing about 200 g, were used for all animal experiments. In vivo, at 24, 48, 72, 96, 144 and 192 h after two- thirds partial hepatectomy （PHx）, the remnant livers were removed. Liver tissues were immunohistochemi- cally stained for Nrp-1, Sema3A and SE-1, a liver sinu- soidal endothelial cell （SEC） marker. Total RNA of the liver tissue was extracted and reversely transcribed into cDNA. The mRNA expression of Sema3A was ana- lyzed by quantitative real-time polymerase chain reac- tion and normalized to that of ribosomal protein $18. In vitro, SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor. Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A. Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method. RESULTS： In vitro, immunohistochemistry study re- vealed that Sema3A and Nrp-1 were constitutively ex- pressed in hepatocytes and SECs, respectively, in normal rat liver tissues. Nrp-1 expression in SECs was quantified by the percentage of immunostained area with anti- Nrp-1 antibody in relation to the area stained with SE-1. Between 24 h and 96 h following resection of liver, Nrp-1 expression in SECs was transiently increased. Compared with the baseline （5.2% ± 0.1%）, Nrp-1 expression in SECs significantly increased at 24 h （17.3% ± 0.7%, P 〈 0.05）, 48 h （39.1% ± 0.6%, P 〈 0.01）, 72 h （46.9% ± 4.5%, P 〈 0.01） and 96 h （29.9% ±3.8%, P 〈 0.01） after PHx, then returned to the basal level at termination of liver regeneration. Interestingly, the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx. Sema3A mRNA expres- sion was significantly reduced by about 75% over the period 24-144 h after PHx （P 〈 0.05）, and returned to basal levels at 192 h after PHx. In vitro, SECs isolated from rats after PHx （PHx-SECs） were observed to mi- grate to the lower chamber of the cell transwell system after incubation for 24 h, but not cells from normal rats （CONT-SECs）, indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs. More- over, recombinant Sema3A significantly attenuated mi- gration in PHx-SECs in primary culture （vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%, P 〈 0.01）, but not in CONT-SECs. Compared with CONT- SECs, the apoptotic rate of PHx-SECs decreased by 78.3% （P 〈 0.05）. There was no difference in apopto- sis between CONT-SECs that were treated with vehicle and Sema3A. However, in PHx-SECs, apoptosis was induced by the presence of 5 nmol Sema3A for 24 h （vehicle-treated 21.7%±7.6% vs Sema3A-treated 104.3% ± 8.9%, P 〈 0.05）. In addition, immunohisto- chemistry confirmed the increased expression of Nrp-1 in PHx-SECs, while it was noted to a lesser extent in CONT-SECs. CONCLUSION： The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understand- ing of interaction between sinusoidal remodeling and SECs during liver regeneration.展开更多
The evoked spike discharges of a neuron depend critically on the recent history of its electrical activity. A well-known example is the phenomenon of spike-frequency adaptation that is a commonly observed property of ...The evoked spike discharges of a neuron depend critically on the recent history of its electrical activity. A well-known example is the phenomenon of spike-frequency adaptation that is a commonly observed property of neurons. In this paper, using a leaky integrate-and-fire model that includes an adaptation current, we propose an event-driven strategy to simulate integrate-and-fire models with spike-frequency adaptation. Such approach is more precise than traditional clock-driven numerical integration approach because the timing of spikes is treated exactly. In experiments, using event-driven and clock-driven strategies we simulated the adaptation time course of single neuron and the random network with spike-timing dependent plasticity, the results indicate that （1） the temporal precision of spiking events impacts on neuronal dynamics of single as well as network in the different simulation strategies and （2） the simulation time in the event-driven simulation strategies. scales linearly with the total number of spiking events展开更多
Ciliary neurotrophic factor (CNTF) has pleiotropic actions on many neuronal populations as well as on glia. Signal transduction by CNTF requires that it bind first to CNTF R, permitting the recruitment of gp130 and L...Ciliary neurotrophic factor (CNTF) has pleiotropic actions on many neuronal populations as well as on glia. Signal transduction by CNTF requires that it bind first to CNTF R, permitting the recruitment of gp130 and LIF R, forming a tripartite receptor complex. Cells that only express gp130 and LIF R, but not CNTF R are refractory to stimulation by CNTF. On many target cells CNTF only acts in the presence of its specific agonistic soluble receptors. We engineered a soluble fusion protein by linking the COOH terminus of sCNTF R to the NH 2 terminus of CNTF. Recombinant CNTF/sCNTF R fusion protein (Hyper CNTF) was successfully expressed in COS 7 cells. The apparent molecular mass of the Hyper CNTF protein was estimated from western blots to be 75 kDa. Proliferation assays of transfected BAF/3 cells in response to CNTF and Hyper CNTF were used to verify the activity of the cytokines. The proliferative results confirmed that CNTF required homodimerization of the gp130, CNTF R and LIF R receptor subunit whereas Hyper CNTF required heterodimerization of the gp130 and LIF R receptor subunit. We concluded that the fusion protein Hyper CNTF had superagonistic activity on target cells expressing gp130 and LIF R, but lacking membrane bound CNTF R.展开更多
Political work is the lifeblood of all economic work, is the core content of universal significance of Mao＇s Economic thought. If, applied broadly, that regarding ideological and political work as the lifeblood of th...Political work is the lifeblood of all economic work, is the core content of universal significance of Mao＇s Economic thought. If, applied broadly, that regarding ideological and political work as the lifeblood of the economy and of all other work, it is an important principle of our Party Marxist revolutionary struggle in the long-term practice of forming a ＂lifeline ＂ was a metaphor, it fully emphasized and affirmed the status and role of ideological and political work, it＇s the concrete application of our party in the dialectical unity of in dialectical material and spiritual, economic and political principles in dialectical naaterialism and historical materialism,it is collective wisdom.展开更多
Vertical hot ring rolling(VHRR) process has the characteristics of nonlinearity,time-variation and being susceptible to disturbance.Furthermore,the ring's growth is quite fast within a short time,and the rolled ri...Vertical hot ring rolling(VHRR) process has the characteristics of nonlinearity,time-variation and being susceptible to disturbance.Furthermore,the ring's growth is quite fast within a short time,and the rolled ring's position is asymmetrical.All of these cause that the ring's dimensions cannot be measured directly.Through analyzing the relationships among the dimensions of ring blanks,the positions of rolls and the ring's inner and outer diameter,the soft measurement model of ring's dimensions is established based on the radial basis function neural network(RBFNN).A mass of data samples are obtained from VHRR finite element(FE) simulations to train and test the soft measurement NN model,and the model's structure parameters are deduced and optimized by genetic algorithm(GA).Finally,the soft measurement system of ring's dimensions is established and validated by the VHRR experiments.The ring's dimensions were measured artificially and calculated by the soft measurement NN model.The results show that the calculation values of GA-RBFNN model are close to the artificial measurement data.In addition,the calculation accuracy of GA-RBFNN model is higher than that of RBFNN model.The research results suggest that the soft measurement NN model has high precision and flexibility.The research can provide practical methods and theoretical guidance for the accurate measurement of VHRR process.展开更多
Objective：To observe the clinical efficacy of skin needling plus heat-sensitive moxibustion in treating chronic facial paralysis. Methods：Sixty patients with chronic facial paralysis were divided into an observation...Objective：To observe the clinical efficacy of skin needling plus heat-sensitive moxibustion in treating chronic facial paralysis. Methods：Sixty patients with chronic facial paralysis were divided into an observation group and an acupuncture group by using the random number table, 30 in each group. Patients in the observation group were given skin needling plus heat-sensitive moxibustion; while patients in the acupuncture group were given conventional acupuncture. Results：Three treatment courses later, the total effective rate was 93.3% in the observation group versus 86.7% in the control group, and the difference was statistically significant （P〈0.05）. Conclusion：Skin needling plus heat-sensitive moxibustion can produce a more significant efficacy than conventional acupuncture in treating chronic facial paralysis.展开更多
Over the course of the epochal 30 years, i.e., from the founding of People＇s Republic of China in 1949 till the launching of Chinese economic reform in 1978, the Communist Party of China （CPC） underwent a tremendou...Over the course of the epochal 30 years, i.e., from the founding of People＇s Republic of China in 1949 till the launching of Chinese economic reform in 1978, the Communist Party of China （CPC） underwent a tremendous transformation from the founding to the ruling party of the country. The present research examines the historical, social as well as political aspects of the identity crisis and the CPC was suffering from in its adventurous efforts to chart a course for the socialist transformation of China. It was tempted to resume its leadership role in the revolution given the mounting tension generated by the Cold War and the prevailing revolutionary beliefs within the Party. Nevertheless, it was obliged to restore the country＇s social and political order after the sweeping national reform. In search of an adequate interpretation for the various attempts the Party made in exploring its refreshed identity, hence resolving the crisis, the study focuses on a similar crisis which befell the Party leader, Mao Zedong, in his own struggle against the con- fusion caused by his potentially double role as the head of both a founding and a ruling party. The study suggests that Mao＇s misidentification of his own political role inevitably led to the Party＇s dysfunctions, and closer investigation into the leader＇s personal dilemma offers new insight into such tragic events as the Anti- Rightist Movement, the Great Leap Forward, and the Cultural Revolution.展开更多
基金Supported by A Grant-in-aid for Young Scientists from Japan Society for the Promotion of Science,No.22790671
文摘AIM： To elucidate the role of neuropilin-1 （Nrp-1） and semaphorin 3A （Sema3A） in sinusoidal remodeling dur- ing liver regeneration in rats. METHODS： Male Wistar/ST rats at 7 wk of age, weigh- ing about 200 g, were used for all animal experiments. In vivo, at 24, 48, 72, 96, 144 and 192 h after two- thirds partial hepatectomy （PHx）, the remnant livers were removed. Liver tissues were immunohistochemi- cally stained for Nrp-1, Sema3A and SE-1, a liver sinu- soidal endothelial cell （SEC） marker. Total RNA of the liver tissue was extracted and reversely transcribed into cDNA. The mRNA expression of Sema3A was ana- lyzed by quantitative real-time polymerase chain reac- tion and normalized to that of ribosomal protein $18. In vitro, SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor. Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A. Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method. RESULTS： In vitro, immunohistochemistry study re- vealed that Sema3A and Nrp-1 were constitutively ex- pressed in hepatocytes and SECs, respectively, in normal rat liver tissues. Nrp-1 expression in SECs was quantified by the percentage of immunostained area with anti- Nrp-1 antibody in relation to the area stained with SE-1. Between 24 h and 96 h following resection of liver, Nrp-1 expression in SECs was transiently increased. Compared with the baseline （5.2% ± 0.1%）, Nrp-1 expression in SECs significantly increased at 24 h （17.3% ± 0.7%, P 〈 0.05）, 48 h （39.1% ± 0.6%, P 〈 0.01）, 72 h （46.9% ± 4.5%, P 〈 0.01） and 96 h （29.9% ±3.8%, P 〈 0.01） after PHx, then returned to the basal level at termination of liver regeneration. Interestingly, the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx. Sema3A mRNA expres- sion was significantly reduced by about 75% over the period 24-144 h after PHx （P 〈 0.05）, and returned to basal levels at 192 h after PHx. In vitro, SECs isolated from rats after PHx （PHx-SECs） were observed to mi- grate to the lower chamber of the cell transwell system after incubation for 24 h, but not cells from normal rats （CONT-SECs）, indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs. More- over, recombinant Sema3A significantly attenuated mi- gration in PHx-SECs in primary culture （vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%, P 〈 0.01）, but not in CONT-SECs. Compared with CONT- SECs, the apoptotic rate of PHx-SECs decreased by 78.3% （P 〈 0.05）. There was no difference in apopto- sis between CONT-SECs that were treated with vehicle and Sema3A. However, in PHx-SECs, apoptosis was induced by the presence of 5 nmol Sema3A for 24 h （vehicle-treated 21.7%±7.6% vs Sema3A-treated 104.3% ± 8.9%, P 〈 0.05）. In addition, immunohisto- chemistry confirmed the increased expression of Nrp-1 in PHx-SECs, while it was noted to a lesser extent in CONT-SECs. CONCLUSION： The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understand- ing of interaction between sinusoidal remodeling and SECs during liver regeneration.
文摘The evoked spike discharges of a neuron depend critically on the recent history of its electrical activity. A well-known example is the phenomenon of spike-frequency adaptation that is a commonly observed property of neurons. In this paper, using a leaky integrate-and-fire model that includes an adaptation current, we propose an event-driven strategy to simulate integrate-and-fire models with spike-frequency adaptation. Such approach is more precise than traditional clock-driven numerical integration approach because the timing of spikes is treated exactly. In experiments, using event-driven and clock-driven strategies we simulated the adaptation time course of single neuron and the random network with spike-timing dependent plasticity, the results indicate that （1） the temporal precision of spiking events impacts on neuronal dynamics of single as well as network in the different simulation strategies and （2） the simulation time in the event-driven simulation strategies. scales linearly with the total number of spiking events
文摘Ciliary neurotrophic factor (CNTF) has pleiotropic actions on many neuronal populations as well as on glia. Signal transduction by CNTF requires that it bind first to CNTF R, permitting the recruitment of gp130 and LIF R, forming a tripartite receptor complex. Cells that only express gp130 and LIF R, but not CNTF R are refractory to stimulation by CNTF. On many target cells CNTF only acts in the presence of its specific agonistic soluble receptors. We engineered a soluble fusion protein by linking the COOH terminus of sCNTF R to the NH 2 terminus of CNTF. Recombinant CNTF/sCNTF R fusion protein (Hyper CNTF) was successfully expressed in COS 7 cells. The apparent molecular mass of the Hyper CNTF protein was estimated from western blots to be 75 kDa. Proliferation assays of transfected BAF/3 cells in response to CNTF and Hyper CNTF were used to verify the activity of the cytokines. The proliferative results confirmed that CNTF required homodimerization of the gp130, CNTF R and LIF R receptor subunit whereas Hyper CNTF required heterodimerization of the gp130 and LIF R receptor subunit. We concluded that the fusion protein Hyper CNTF had superagonistic activity on target cells expressing gp130 and LIF R, but lacking membrane bound CNTF R.
文摘Political work is the lifeblood of all economic work, is the core content of universal significance of Mao＇s Economic thought. If, applied broadly, that regarding ideological and political work as the lifeblood of the economy and of all other work, it is an important principle of our Party Marxist revolutionary struggle in the long-term practice of forming a ＂lifeline ＂ was a metaphor, it fully emphasized and affirmed the status and role of ideological and political work, it＇s the concrete application of our party in the dialectical unity of in dialectical material and spiritual, economic and political principles in dialectical naaterialism and historical materialism,it is collective wisdom.
基金Project(51205299)supported by the National Natural Science Foundation of ChinaProject(2015M582643)supported by the China Postdoctoral Science Foundation+2 种基金Project(2014BAA008)supported by the Science and Technology Support Program of Hubei Province,ChinaProject(2014-IV-144)supported by the Fundamental Research Funds for the Central Universities of ChinaProject(2012AAA07-01)supported by the Major Science and Technology Achievements Transformation&Industrialization Program of Hubei Province,China
文摘Vertical hot ring rolling(VHRR) process has the characteristics of nonlinearity,time-variation and being susceptible to disturbance.Furthermore,the ring's growth is quite fast within a short time,and the rolled ring's position is asymmetrical.All of these cause that the ring's dimensions cannot be measured directly.Through analyzing the relationships among the dimensions of ring blanks,the positions of rolls and the ring's inner and outer diameter,the soft measurement model of ring's dimensions is established based on the radial basis function neural network(RBFNN).A mass of data samples are obtained from VHRR finite element(FE) simulations to train and test the soft measurement NN model,and the model's structure parameters are deduced and optimized by genetic algorithm(GA).Finally,the soft measurement system of ring's dimensions is established and validated by the VHRR experiments.The ring's dimensions were measured artificially and calculated by the soft measurement NN model.The results show that the calculation values of GA-RBFNN model are close to the artificial measurement data.In addition,the calculation accuracy of GA-RBFNN model is higher than that of RBFNN model.The research results suggest that the soft measurement NN model has high precision and flexibility.The research can provide practical methods and theoretical guidance for the accurate measurement of VHRR process.
文摘Objective：To observe the clinical efficacy of skin needling plus heat-sensitive moxibustion in treating chronic facial paralysis. Methods：Sixty patients with chronic facial paralysis were divided into an observation group and an acupuncture group by using the random number table, 30 in each group. Patients in the observation group were given skin needling plus heat-sensitive moxibustion; while patients in the acupuncture group were given conventional acupuncture. Results：Three treatment courses later, the total effective rate was 93.3% in the observation group versus 86.7% in the control group, and the difference was statistically significant （P〈0.05）. Conclusion：Skin needling plus heat-sensitive moxibustion can produce a more significant efficacy than conventional acupuncture in treating chronic facial paralysis.
文摘Over the course of the epochal 30 years, i.e., from the founding of People＇s Republic of China in 1949 till the launching of Chinese economic reform in 1978, the Communist Party of China （CPC） underwent a tremendous transformation from the founding to the ruling party of the country. The present research examines the historical, social as well as political aspects of the identity crisis and the CPC was suffering from in its adventurous efforts to chart a course for the socialist transformation of China. It was tempted to resume its leadership role in the revolution given the mounting tension generated by the Cold War and the prevailing revolutionary beliefs within the Party. Nevertheless, it was obliged to restore the country＇s social and political order after the sweeping national reform. In search of an adequate interpretation for the various attempts the Party made in exploring its refreshed identity, hence resolving the crisis, the study focuses on a similar crisis which befell the Party leader, Mao Zedong, in his own struggle against the con- fusion caused by his potentially double role as the head of both a founding and a ruling party. The study suggests that Mao＇s misidentification of his own political role inevitably led to the Party＇s dysfunctions, and closer investigation into the leader＇s personal dilemma offers new insight into such tragic events as the Anti- Rightist Movement, the Great Leap Forward, and the Cultural Revolution.
