目的建立超高效液相色谱-串联质谱法(ultra high performance liquid chromatography tandem mass spectrometry,UPLCMS/MS)检测毛蕊花糖苷干预不育症模型大鼠血浆睾酮的含量,测定不育症模型大鼠血浆24 h睾酮含量,并借助药动学理论定量...目的建立超高效液相色谱-串联质谱法(ultra high performance liquid chromatography tandem mass spectrometry,UPLCMS/MS)检测毛蕊花糖苷干预不育症模型大鼠血浆睾酮的含量,测定不育症模型大鼠血浆24 h睾酮含量,并借助药动学理论定量评价干预效果。方法大鼠含药血浆以睾酮-C3为内标,用液液萃取法,以12000 r·min^(-1)离心10 min,取上清液,5μL进样分析。以Acquity UPLCⅢBEH C_(18)(50 mm×2.1 mm,1.7μm)为色谱柱;以甲醇为流动相A、5 mL·L^(-1)甲酸溶液为流动相B进行梯度洗脱,洗脱程序:流速为0.2 mL∙min^(-1);柱温为35℃;样品室温度为4℃;运行时间为10 min;质量检测器使用ESI离子源,正模式检测,睾酮及内标睾酮-C3用于定量的母→子离子对分别为m/z 289.2→97.0、m/z 291.4→101.1。借助药动学软件分析,获取空白组和毛蕊花糖苷干预组大鼠体内24 h睾酮曲线下面积(area under the curve,AUC)数据。结果睾酮质量浓度在1~1000 ng·mL^(-1)范围内呈良好的线性关系。该方法日内、日间精密度RSD值均<10.84%,日内、日间准确度RE均<10.03%,样品提取回收率为95.82%~99.17%,置于进样器于4℃冰箱及长期冷冻稳定性RSD值均<10.63%。测定结果显示,毛蕊花糖苷干预组药峰浓度(peak concentration,C_(max))为7.92 ng·mL^(-1),空白组C_(max)为10.26 ng·mL^(-1)。与空白组比较,毛蕊花糖苷干预组大鼠体内睾酮24 h总AUC相对平稳。结论建立了UPLC-MS/MS测定不育症模型大鼠血浆睾酮含量的方法。从PK/PD角度比较分析推测出,给予毛蕊花糖苷干预后,不育症模型大鼠的24 h总体睾酮可基本恢复正常水平,从而发挥改善大鼠雄性生殖功能的作用。展开更多
毛蕊花糖苷(Verbascoside, VB),作为苯乙醇苷类多酚化合物中的代表性成分,广泛存在于肉苁蓉等多种药用植物中,具备丰富的生物活性。现代药理研究表明,VB不仅在抗炎、抗氧化、神经保护、抗癌上效果显著,还能抗菌,调节免疫系统,保护肝脏...毛蕊花糖苷(Verbascoside, VB),作为苯乙醇苷类多酚化合物中的代表性成分,广泛存在于肉苁蓉等多种药用植物中,具备丰富的生物活性。现代药理研究表明,VB不仅在抗炎、抗氧化、神经保护、抗癌上效果显著,还能抗菌,调节免疫系统,保护肝脏、肾脏和肺,缓解疲劳,促进伤口愈合,对多种疾病具有潜在的治疗价值。本文综述了近年来关于VB的药理活性和作用机制的研究,旨在为VB的深入研究与应用提供参考。Verbascoside (VB), a representative phenylethanoid glycoside polyphenolic compound, is abundantly found in various medicinal plants such as Cistanches Herba. Extensive biological activities have been documented. Modern pharmacological studies reveal that VB not only significantly benefits anti-inflammatory, antioxidant, neuroprotective, and anticancer activities, but also possesses antimicrobial properties, and protects the livers, kidneys and lungs. It alleviates fatigue and promotes wound healing, offering potential therapeutic value for multiple diseases. This paper reviews recent research on the pharmacological activities and mechanisms of action of VB, aiming to provide references for further research and application of VB.展开更多
文摘毛蕊花糖苷(Verbascoside, VB),作为苯乙醇苷类多酚化合物中的代表性成分,广泛存在于肉苁蓉等多种药用植物中,具备丰富的生物活性。现代药理研究表明,VB不仅在抗炎、抗氧化、神经保护、抗癌上效果显著,还能抗菌,调节免疫系统,保护肝脏、肾脏和肺,缓解疲劳,促进伤口愈合,对多种疾病具有潜在的治疗价值。本文综述了近年来关于VB的药理活性和作用机制的研究,旨在为VB的深入研究与应用提供参考。Verbascoside (VB), a representative phenylethanoid glycoside polyphenolic compound, is abundantly found in various medicinal plants such as Cistanches Herba. Extensive biological activities have been documented. Modern pharmacological studies reveal that VB not only significantly benefits anti-inflammatory, antioxidant, neuroprotective, and anticancer activities, but also possesses antimicrobial properties, and protects the livers, kidneys and lungs. It alleviates fatigue and promotes wound healing, offering potential therapeutic value for multiple diseases. This paper reviews recent research on the pharmacological activities and mechanisms of action of VB, aiming to provide references for further research and application of VB.
