Effects of Different Water Stresses on Eco-physiological Characteristics of Hippophae rhamnoides Seedlings

In order to examine the effects of the decrease of future precipitation on the eco-physiological characteristics of sea buckthorn (Hippophae rhamnoides Linn.) in Huangfuchuan Watershed in Nei Mongol, a water gradient experiment was conducted based on the four specially designed water supply levels, including normal precipitation, slight drought, drought and extreme drought. Results of ANOVE showed that different water gradients had a significant effect on (1) microhabitat factors, such as soil water content and soil temperature; (2) gas exchange, such as net photosynthetic rate, stomatal conductance and transpiration rate; (3) resource use efficiency; and (4) leaf water potential. Water use efficiency of H rhamnoides could increase under moderate water stress, i.e. drought condition, while its net photosynthetic rate and transpiration rate decreased. All kinds of eco-physiological characteristics proved H. rhamnoides seedlings under all water supplies were affected by water stress more or less and that mechanism of intrinsic physiological regulation in seedlings under the extreme drought conditions had the appearance of turbulence to a certain extent. Therefore, H rhamnoides seedlings in Huangfuchuan Watershed could not acclimate to extreme drought conditions.

Effects of water stress on Haloxylon ammodendron seedlings in the desert region of Heihe inland river watershed, Gansu Province, China

The water relation and leaf gas exchange of saxoul (Haloxylon Ammodendron Bge, a C4 shrub) seedlings were studied under water stress in 2001. Saxoul seedlings maintained high transpiration when the soil moisture was above 11%. The seedlings were able to take up water from soil with above 6 % soil water content, which was the threshold level of soil moisture for seedlings. The relationship between transpiration and potential evaporation was linear for well-watered seedlings. The de-crease of soil water availability led to different degrees of down-regulation of stomatal conductance, leaf transpiration and net CO2 assimilation rate. The stomata played a relatively small part in determining the net CO2 assimilation rate for the same seedling. The relationship between net CO2 assimilation rate and transpiration was linear diurnally, and reduction scale of leaf transpiration was much bigger than that of net CO2 assimilation rate by waters tress treatments, therefore intrinsic wa-ter-use-efficiency increased. High evaporative demand increased the leaf transpiration but inhibited net CO2 assimilation rate. Because of the effect of VPD on transpiration in this region, the transpiration of well-watered and mild water stress seedlings becomes responsive to change in stomatal conductance over a wider range.

Analysis and Evaluation Indicator Selection of Chilling Tolerance of Different Cotton Genotypes

[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively high photo- synthetic electron transport capacity and high stomatal conductance after recovery treatment, which is contributed to gas exchange and recovery of photosynthetic ca- pacity. Determination of the six indicators under the same stress condition can be adopted for rapid identification and prediction of the chilling tolerance of other cotton varieties, which provides basis for the breeding, promotion, identification and screen- ing of chilling tolerant germplasms.

Tempo-Spatial Variations in Stomatal Conductance, Aperture and Density of Ligustrum sinense Acclimated to Different Light Environments

There was heterogeneous distribution in stomatal conductance (g-s) and stomatal aperture for both high- and low-light leaves of Ligustrum sinense Lour. in four designated positions within a leaf. Linear or exponential or polynomial relationships between g-s and stomatal aperture were found when regression of g-s and stomatal aperture was established. Statistical analysis revealed that the relationship between g-s and stomatal aperture for high-light leaves was more significant than that of low-light leaves. A linear relationship between g-s and stomatal aperture existed in both positions 1 and 3 for both high- and low-light leaves. The stomatal density of the low-light leaves was much lower than that of the high-light leaves. The sensitivity of stomata to changing environment for high-light leaves was higher than that for low-light leaves, which may also relate to the higher stomatal density for the high-light leaves.

Photosynthetic Gas Exchange and Leaflet Movement of Robinia pseudoacacia in Relation to Changing Light Environments

On the sunny days, there were little diurnal changes in both azimuth and directional angle for either sun_ or shade_leaflet. However, there existed a significant diurnal change in midrib angle that reflected movements regarding evasion of light stress around noon hours. On the cloudy day, a very little diurnal difference was found in azimuth, directional and midrib angle. It is suggested that changing light environment is the main factor for inducing leaflet movement of Robinia pseudoacacia , and the rhythmical movement does not attribute to the leaflet movement. Leaf orientation control test showed that the photosynthetic rate ( Pn ), stomatal conductance ( g s ) and transpiration ( Tr ) of the artificial fixed_leaflets were significantly lower than that of control_leaflets. And the leaf temperature ( Tl ) of the fixed leaflets significantly exceeded that of control_leaflets, which may attribute to the significant difference of light interception between the fixed and control_leaflets. The light_dependent leaflet movement is the morphological adjustment of maintaining optimal physiological status.

Model of gas exchange dynamics for modified-atmosphere packages containing fresh produce

A model for modified-atmosphere packaging （MAP） systems containing fruits and vegetables was developed.The computer simulation was performed to predict the gas mass concentrations inside the packages and was successfully verified by experiments with yellow peaches at 5,15 and 25 ℃ using two types of packaging films.A Michaelis-Menten type respiration model with noncompetitive inhibition mechanism due to CO2 was adopted while the respiration rates were measured with an improved permeable system method suitable for either steady or unsteady state.The applicability of the model in the design of MAP systems was demonstrated with a calculation to evaluate film specification and equilibrium concentrations of O2 and CO2 in the package containing yellow peaches.

Identification of Plasma Membrane Aquaporin in Guard Cells of Vicia faba and Its Role in Stomatal Movement

Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in die guard cells of Vicia faba L. were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in ale mesophyll cells and vascular tissues of V. faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25 mumol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guardcell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L, beta-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 Pretreatment oil stomatal opening ( i.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. faba are associated with stomatal movement.

Changes of Gas Exchanges in Leaves of Different Cultivars of Winter Wheat Released in Different Years

Three winter wheat cultivars ( Triticum aestivum L.), representatives of those widely cultivated in Beijing over the past six decades, were grown in the same environmental condition, and their physiological features were investigated. Daily changes of net photosynthetic rate (P-n), transpiration (T-r) in different growth stages were measured in order to find the relationship between leaf photosynthesis and yield. Instantaneous water use efficiency (WUE) of leaf was calculated from P-n/T-r. It is suggested that relationship between photosynthetic rate and yield changed with the developing stages of wheat. High yield wheat cultivar Jingdong 8 (released in the 1990s) had a higher photosynthetic rate ( the maximal P-n increased by 77%) and transpiration rate (the maximal T-r increased by 69%), but a lower WUE than the low yield cultivar Yanda 1817 (released in the 1940s) during the day time at stem elongation stage. However; difference of P-n among the three cultivars changed with wheat growth process. Before 10 o'clock P-n in leaves of Jingdong 8 usually was the highest of the three cultivars, but P-n of Yanda 1817 was the highest after 10 o'clock. At dough ripe stage, P-n in leaves of Yanda. 1817 was the highest among the three cultivars during the whole day. The difference of changing trend of transpiration in three wheat cultivars was similar to P,, but WUE of Yanda 1817 was the highest in those three cultivars, indicating that the higher yield of Jingdong 8 was achieved via a greater consumption of water. Contrary to the cultivars released in the later period, midday depression of photosynthesis was small in Yanda 1817, which might suggest that Yanda 1817 was resistant to photoinhibition. It is possible that photosynthetic potential in leaves of wheat increased as wheat cultivars was improved over the past six decades. However, it became less resistant to photoinhibition.

Ionic Conduction and Fuel Cell Performance of Ba0.98Ce0.8Tm0.2O3-α Ceramic

The perovskite-type oxide solid solution Ba0.98Ce0.8Tm0.2O3-α was prepared by high temperature solid-state reaction and its single phase character was confirmed by X-ray diffraction. The conduction property of the sample was investigated by alternating current impedance spectroscopy and gas concentration cell methods under different gases atmospheres in the temperature range of 500-900 ℃. The performance of the hydrogen-air fuel cell using the sample as solid electrolyte was measured. In wet hydrogen, the sample is a pure protonic conductor with the protonic transport number of 1 in the range of 500-600 ℃, a mixed conductor of proton and electron with the protonic transport number of 0.945-0.933 above 600 ℃. In wet air, the sample is a mixed conductor of proton, oxide ion, and electronic hole. The protonic transport numbers are 0.010-0.021, and the oxide ionic transport numbers are 0.471-0.382. In hydrogen-air fuel cell, the sample is a mixed conductor of proton, oxide ion and electron, the ionic transport numbers are 0.942 0.885. The fuel cell using Ba0.98Ce0.8Tm0.2O3-α as solid electrolyte can work stably. At 900 ℃, the maximum power output density is 110,2 mW/cm2, which is higher than that of our previous cell using Ba0.98Ce0.8Tm0.2O3-α （x〈≤1, RE=Y, Eu, Ho） as solid electrolyte.

用三心来提升生物课堂幸福度

教育应该是幸福完整的生活,教育的目的就是使人幸福.生物课堂的幸福感教育是一种教学实践和追求,在追求中让学生感受和获得幸福,在幸福的状态中健康快乐地生活和成长,从而达到教育的目的.在生物课堂教学中,用心教育就是一种幸福,也是一扇门,推开它,看到的满是阳光和鲜花,给人带来愉悦和幸福,以此来提升生物课堂的幸福度.一、爱心-温暖留守儿童,体验父母关爱城市化建设的飞快发展,大量的农民工涌入城市。

Effect of Shading on Gas Exchange of Cotton Leaves Under Conditions of Different Soil Water Contents

Effect of different shading levels (no-shading, 80% shading, and 40% shading) on photosynthetic and stomatal responses in cotton leaves were investigated under conditions of different soil water contents in summer midday. All cotton leaves exhibited similar basic responses to shading, including decreased net photosynthetic rates, a tendency to decrease in transpiration rates, and increased stomatal conductance and intercellular CO2 concentration. The leaf conductance of 80% shaded and 40% shaded plants increased by 28% and 16.7% compared with no-shaded plants at high water, respectively, but the net photosynthetic rates of 80% shaded and 40% shaded plants declined by 50% and 14.73%, respectively. Results showed that combined effect of soil moisture and shading on photosynthetic and stomatal responses in cotton leaves was very remarkable.

Urgent Tracheal Resection and Reconstruction Assisted by Temporary Cardiopulmonary Bypass:a Case Report

SEVERE tracheal stenosis can not only cause criti- cal medical problems such as severe shortness of breath, hypoxia, and even orthopnea, but also impose overwhelming challenges on thephysicians, particularly the anesthesiologist. Life-threa- tening airway obstruction can make the patient＇s gas ex- change extremely difficult. Though several options could be offered regarding the treatment of tracheal stenosis, normally, tracheal resection and following reconstruction is the first choice for severe airway stenosis.1 Successful surgical intervention relies on the close communication and cooperation between surgeons and anesthesiologists.

Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased sig- nificantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types, although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

Cotton Physiological Parameters Affected by Episodic Irrigation Interruption

Improving cotton irrigation management practices in West Texas is important for increasing farmers＇ profits and for sustainability of the Ogallala aquifer. The objective of this work was to evaluate the effects in field controlled episodic drought conditions on cotton gas exchange. Irrigated cotton was subjected to water stress at different timings. Irrigation was interrupted at the squaring stage, early flowering stage, from three weeks at peak bloom, and from peak bloom to the crop termination. These episodic drought treatments were compared with cotton fully irrigated throughout the whole season. From 2010 to 2012, cotton cultivar FM9180 gas exchange was measured throughout the season using a LiCor-6400 portable photosynthesis system. In 2011 and 2012, measurements were also made on DP0935 cultivar. The cotton physiological parameters evaluated included photosynthesis, transpiration and temperature. From the several parameters evaluated, some relationships were presented. Episodic drought periods can affect leaf-level gas exchange and impact yield. Photosynthesis and yield were particularly sensitive to water deficit at early flowering. Despite an increase in leaf water use efficiency under water deficit, overall growth and yield were inhibited in all treatments with a stress component. Understanding the relative sensitivity at different growth stages can help with irrigation decisions when water resources are limited.

Bubble Formation Characteristics from a Sieve Tray with Liquid Cross-flow

An apparatus, designed to simulate bubbling of sieve tray operatedin froth regime, was employed. Bubble contact angles in and above theincipient weeping regime for an air-water-plexiglas system wereinvestigated. The influence of both liquid cross-flow and gas up-flowupon bubble contact angles was examined. A model consider- Ing theinfluence of liquid cross-flow was developed to predict bubble sizefrom a sieve hole in froth operation regime. The comparison showsthat the bubble sizes predicted by the present model are consistentwith our experimental Values and the available published experimentaldata.

Assessment of achievements of the Lima Climate Change Conference and perspectives on the future

The Lima call for climate action adopted at the Lima Climate Conference on Climate Change specifies that the principles of the United Nations Framework Convention on Climate Change,including the principle of common but differentiated responsibilities,shall apply to the new climate agreement to be adopted at the Paris Conference on Climate Change in 2015.Decisions on other heavily debated items,including the intended nationally determined contributions,were also made at the Lima Conference.The significant achievements in Lima and the positive momentum have laid a solid foundation for the adoption of a new climate agreement in the Paris Climate Conference.Four measures are proposed for China to meet great challenges in addressing climate change beyond 2020,including early formulation and issuance of a climate change law,establishment of a greenhouse gas emission trading scheme,promotion of advanced climate technology investments,and further international engagement for climate change.

Determinations of Air Tightness of Covered Slurry Storage Tanks Using Tracer Gas Technique

The objective of this investigation was to study whether it is possible to determine the air tightness of covered slurry storage tanks using tracer gas technique by injection of sulphur hexafluoride （SF6） and measurements of the decay in gas concentrations with an infrared spectrophotometer in the air space above the slurry. By measuring the decay in concentration of injected tracer gas at different occasions, the air exchange rate was determined. Three different types of coverings were studied namely; wooden roof, plastic tent and concrete roof.

Three-dimensional transient numerical simulation for gas exchange process in a four-stroke motorcycle engine

Three-dimensional transient numerical simulation of gas exchange process in a four-stroke motorcycle engine with a semi-spherical combustion chamber with two tilt valves was studied. Combination of the grid re-meshing method and the snapper technique made the valves move smoothly. The flow structure and pattern in a complete engine cycle were described in detail. Tumble ratios around the x-axis and y-axis were analyzed. Comparison of computed pressure with experimental pressure under motored condition revealed that the simulation had high calculation precision; CFD simulation can be regarded as an im-portant tool for resolving the complex aerodynamic behavior in motorcycle engines.

Urban transport policy response to global climate change in China

Accompanying with the economic development and the process of industrialization and urbanization in China, the energy consumption and greenhouse gases emission of transport sector increased rapidly. From the viewpoint of how the urban traffic management department should cope with the global climate change, based on the economy, institutional reform, planning and policies in China, the paper analyzed the main important problems and obstacles in the development of urban transport, put forward the corresponding policy recommendations which could lead the urban transport system transform to low carbon emission and become more suitable to the climate change.

Remaining oil distribution in models with different heterogeneities after CO_(2) WAG injection:Visual research by nuclear magnetic resonance technique

Water alternating gas(WAG)injection is a widely used strategy for enhancing oil recovery(EOR)during gas flooding,and the mechanisms,operating parameters,and influencing factors of which have been extensively studied.However,with respect to its capacity in expanding macroscopic sweep volume under varying heterogeneities,the related results appear inadequate.In this research,three cores with different heterogeneities were used and flooded by the joint water and CO_(2) WAG,then the effects of heterogeneity on oil recovery were determined.More importantly,the cores after CO_(2) WAG injection were investigated using the nuclear magnetic resonance(NMR)technique for remaining oil distribution research,which could help us to understand the capacity of CO_(2) WAG in enlarging sweep volume at different heterogeneities.The results show that the presence of heterogeneity may largely weaken the effectiveness of water flooding,the more severe the heterogeneity,the worse the water flooding.The WAG injection of CO_(2) performs well in EOR after water flooding for all the cores with different heterogeneities;however,it could barely form a complete or full sweep throughout the low-permeability region,and un-swept bypassed regions remain.The homogeneous core is better developed by the injection of the joint water and CO_(2) WAG than the heterogeneous and fractured cases.