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不同气干时间大叶榉木材DNA提取及ISSR-PCR扩增方法研究 被引量:1
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作者 刘秭攸 骆嘉言 蒋泽平 《安徽大学学报(自然科学版)》 CAS 北大核心 2019年第3期72-79,共8页
大叶榉是我国历史悠久的家具用材.为了找出适用于不同气干时间大叶榉(Zelkova schneideriana)木材的DNA提取方法,用6种不同的提取方法对不同气干时间的大叶榉木材进行了DNA提取,并检测了DNA的数量和质量,而后进行ISSR-PCR的扩增.结果表... 大叶榉是我国历史悠久的家具用材.为了找出适用于不同气干时间大叶榉(Zelkova schneideriana)木材的DNA提取方法,用6种不同的提取方法对不同气干时间的大叶榉木材进行了DNA提取,并检测了DNA的数量和质量,而后进行ISSR-PCR的扩增.结果表明:CTAB-SDS法或CTAB法提取浓度最高,试剂盒法提取浓度最低;4a内气干时间的大叶榉木材使用CTAB-SDS法就可获得合格的DNA,长期气干的需用PTB法;引物836对大叶榉木材DNA分子片段扩增效果最好,对长期气干大叶榉标本可扩增出5条条带,长度达到1 500bp;CTAB-SDS法和PTB法适合进行大叶榉气干材DNA的提取. 展开更多
关键词 大叶榉 气干时间 DNA提取 ISSR-PCR
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Experimental Investigation on Simultaneous Dry Blanching and Drying of Mushroom Slices Using Microwave Enhanced Hot Air Heating System 被引量:1
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作者 Ipsita Das Girish Kumar 《Journal of Food Science and Engineering》 2013年第10期517-524,共8页
The study evaluated the feasibility of using microwave enhanced hot air heating system for simultaneous dry blanching and dehydration of mushroom slices. The technology involves application of microwave energy at the ... The study evaluated the feasibility of using microwave enhanced hot air heating system for simultaneous dry blanching and dehydration of mushroom slices. The technology involves application of microwave energy at the beginning of dehydration process to inactivate enzymes as well as to remove a certain amount of moisture at the same time and then followed by hot air drying to complete the process. The study investigated effects of different processing parameters, i.e., microwave power level and exposure time on dehydration and quality characteristics of mushroom slices. Mushroom slices were pretreated with different microwave power levels of 240, 360 and 480 W for 1, 3 and 5 min before the hot air-drying. The optimum range of the microwave power level and pretreatment time was found to be 360 W, 3 min and 360 W, 1 min in obtaining the maximum and minimum levels of response parameters. 展开更多
关键词 DEHYDRATION dry blanching MICROWAVE mushroom slices.
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Research on Cassava Drying, Application and Promotion of the Reversible Airflow Flatbed Dryers for Sliced Cassava
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作者 Tran Van Tuan Le Quang Vinh Nguyen Van Xuan 《Journal of Food Science and Engineering》 2015年第3期150-158,共9页
The study was conducted by researchers of the Research Center for Agricultural Energy and Machinery (CAEM) of the Ho Chi Minh City Nong Lam University (NLU) with the objectives of researching on drying ways for ca... The study was conducted by researchers of the Research Center for Agricultural Energy and Machinery (CAEM) of the Ho Chi Minh City Nong Lam University (NLU) with the objectives of researching on drying ways for cassava and of evaluating the adaptability of the reversible-airflow dryers in drying of cassava. The results obtained were as follows: done with thin-layer drying experiments, deep-layer drying experiments in the lab, and conducted drying experiments at the cassava dryer in actual production scale with two capacity sizes of 8 tons/batch and 16 tons/batch, that operates based on principle of reversible-airflow drying (SRA dryers). By the means of drying experiments, the performance of the SRA dryers such as optimal drying temperature, drying time, drying airflow reversal timing, moisture content uniformity of the sliced cassava mass after finishing drying process, drying cost, etc. was defined, the optimal drying temperature was 70 ~C, the total actual drying time for finishing one batch was 25-28 hours, applicable time for drying airflow reversal was after drying 16 hours since startup, the deviation in moisture content of the cassava mass after drying was just 2%-3%, the drying costs (per one kg of dried cassava) calculated were in turns VND407/kg at the SRA-8 dryer (8 tons/batch), and VND351/kg at the SRA-16 dryer (16 tons/batch). Obviously, comparing with the sale price of dry cassava at present, these levels of drying cost are accounting for 7%-10%, which are suitable and acceptable. Thus, investment of the dryers for sliced cassava would be effective and contribute to bring more income for Vietnamese cassava farmers and dryer end-users. 展开更多
关键词 CASSAVA sliced cassava SRA dryer wood-chip furnace.
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