渤海油田渤中13-1南区块馆陶组储集层流体性质识别方法研究

为了解决渤海油田渤中13-1南区块油质复杂、流体类型识别难度大的问题,通过统计气测资料、测井资料和试油资料,对该区块馆陶组不同流体类型气测组分及其派生参数和荧光显示特征进行了研究,建立了馆陶组气测-荧光解释评价标准,同时利用地化参数及其派生参数建立了地化亮点图板。应用气测-荧光解释评价标准与地化亮点图板相结合,对目前已钻预探井进行解释评价,结果表明,解释层数82层,其中74层符合,解释符合率为90.2%,对该区块新钻井进行现场综合解释,解释14层,其中12层符合,综合解释符合率达到85.7%。该方法大幅提高了现场解释符合率,为下一步勘探开发决策提供了有利依据。

Effect of Yiqiyangyin recipe on heparanase and nephrin in rats with adriamycin-induced nephropathy

OBJECTIVE： To discuss the mechanism of Yiqiyang- yin recipe in rats with adriamycin （ADR）-induced ne- phropathyo METHODS： We randomly divided 30 Sprague-Daw- ley rats into 5 groups： control, model, glucocorti- coid, Chinese herb, and Chinese herb plus glucocor- ticoid groups. The 24-h urine volume was collected on days 7, 14, 21, and 28 after ADR injection, and all rats were killed on day 28. We measured the 24-h levels of urinary protein, serum cholesterol, and se- rum triglycerides, and renal function of all rats by using routine biochemical methods. Pathological changes in the rat kidneys were observed under light and electron microscopes. Heparanase （HPA） mRNA expression levels were measured using re-al-time fluorescence-quantitative polymerase chain reaction. Urine levels of HPA in all groups were measured using enzyme-linked immunosor- bent assay. The expression of nephrin was detected by immunohistochemical staining and quantitative- ly analyzed using Motic image analysis 3.2 software. RESULTS： The levels of urinary protein and serum triglycerides and cholesterol in rats with ADR-in- duced nephropathy increased on day 14. The se- rum albumin levels simultaneously decreased. All the changes reached the peak on day 28. Examina- tion under the light microscope showed inflamma- tory cells and slight fibroplasia in the renal intersti- tium in the model group, but fewer inflammatory cells were observed in the intervention groups than those in the model group. Examination under the electron microscope showed extensive fusion of foot processes in ADR rats. HPA mRNA expres- sion was higher in the model group than that in the control group. The HPA mRNA levels in the in- tervention groups, especially in the Chinese herb group, and Chinese herb plus glucocorticoid group were significantly lower than the level in the model group. The HPA expression levels correlated signifi- cantly with the proteinuria level. No significant dif- ference was found in the HPA level in urine be- tween the intervention groups and the model group, whereas the model group had a higher uri- nary HPA level than the control group. Nephrin mRNA expression levels in the model group were higher than those in the control group. Nephrin mRNA expression levels were significantly lower in the intervention groups than that in the model group, especially the Chinese herb plus glucocorti- cold group. Compared with the control group, themodel group showed increased nephrin expression in the kidney. Nephrin levels in the other groups, es- pecially in the Chinese herb plus glucocorticoid group, were significantly lower than that in the model group. The nephrin levels in the kidney were negatively correlated with the proteinuria level. CONCLUSION： Yiqiyangyin recipe could attenuate foot process injury particularly in combination with a steroid reduce the development of proteinuria possibly by inhibition of HPA in the kidney, and reg- ulate the expression of nephrin in rats with ADR-in- duced nephropathy. Our study showed that treat- ment with Yiqiyangyin recipe plus glucocorticoid was better than a singular intervention, and we ex- plored the pharmacological mechanism of this combination by biochemical and molecular biologi- cal analysis to provide a basis for clinical applica- tion.