ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentr...ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was （37.04±6.43） %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol.展开更多
The objectives of this work are to study the primary chemical structure of soot aerosol derived from lump-coal combustion in different experimental conditions in fixed bed. A laboratory-scale movable fixed bed, water-...The objectives of this work are to study the primary chemical structure of soot aerosol derived from lump-coal combustion in different experimental conditions in fixed bed. A laboratory-scale movable fixed bed, water-cooled soot aerosol collection system, and electric reactor have been designed and used in the process. Three kinds of coals, sized at 3-5 ram, have been heated in the experiments. Fourier Transform Infrared Spectroscopy （FTlR） has been employed to test functional groups of soot aerosol samples. Infrared spectra from 400 to 4000 cm^ -1 and semiquantitative analysis have been employed. The results of experiments show that contents of hydrogen-bonded are increased, contents of unsaturated hydrocarbons are increased, and contents of aromatic hydrocarbons are decreased with temperature increase; contents of hydrogen-bonded and unsaturated hydrocarbons are increased first and decreased late, and contents of aromatic hydrocarbons are decreased with gases residence time extension; the contents of hydrogen bonded and unsaturated hydrocarbons derived from soot aerosol samples are higher than those from original coal samples in lower-volatile coals, and the contents of aromatic hydrocarbons derived from soot aerosol samples are lower than those from original coals; and contents of hydrogen-bonded are decreased, contents of unsaturated hydrocarbons are increased, and contents of aromatic hydrocarbons are decreased with a increase.展开更多
基金Supported by the Fund for Agricultural Science and Technology Independent Innovation of Jiangsu Province[CX(12)1001-05]~~
文摘ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae （Mhp） in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was （37.04±6.43） %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol.
文摘The objectives of this work are to study the primary chemical structure of soot aerosol derived from lump-coal combustion in different experimental conditions in fixed bed. A laboratory-scale movable fixed bed, water-cooled soot aerosol collection system, and electric reactor have been designed and used in the process. Three kinds of coals, sized at 3-5 ram, have been heated in the experiments. Fourier Transform Infrared Spectroscopy （FTlR） has been employed to test functional groups of soot aerosol samples. Infrared spectra from 400 to 4000 cm^ -1 and semiquantitative analysis have been employed. The results of experiments show that contents of hydrogen-bonded are increased, contents of unsaturated hydrocarbons are increased, and contents of aromatic hydrocarbons are decreased with temperature increase; contents of hydrogen-bonded and unsaturated hydrocarbons are increased first and decreased late, and contents of aromatic hydrocarbons are decreased with gases residence time extension; the contents of hydrogen bonded and unsaturated hydrocarbons derived from soot aerosol samples are higher than those from original coal samples in lower-volatile coals, and the contents of aromatic hydrocarbons derived from soot aerosol samples are lower than those from original coals; and contents of hydrogen-bonded are decreased, contents of unsaturated hydrocarbons are increased, and contents of aromatic hydrocarbons are decreased with a increase.
