In recent years,oil spill accidents occur frequently in the marine area of China.Finding out the spilled oil source is a key step in the relevant investigation.In this paper,a step-by-step fingerprinting identificatio...In recent years,oil spill accidents occur frequently in the marine area of China.Finding out the spilled oil source is a key step in the relevant investigation.In this paper,a step-by-step fingerprinting identification method was used in a spilled oil accident in the Bohai Sea in 2002.Advanced chemical fingerprinting and data interpretation techniques were used to characterize the chemical composition and determine the possible sources of two spilled oil samples.The original gas chromatography -flame ionization detec-tion (GC-FID) chromatogram of saturated hydrocarbons was compared.The gas chromatography-mass spectrometry (GC/MS) chromatograms of aromatic hydrocarbons terpane and sterane,n-alkane and poly-aromatic hydrocarbons (PAHs) were analyzed.The correlation analysis on diagnostic ratios was performed with Student’s t-test.It is found that the oil fingerprinting of the spilled oil (designated as sz1) from the polluted sand beach was identical with the suspected oil (designated as ky1) from a nearby crude oil refinery factory.They both showed the fingerprinting character of mixed oil.The oil fingerprinting of the spilled oil (designated as ms1) collected from the port was significantly different from oil ky1 and oil sz1 and was with a lubricating oil fingerprint character.The identification result not only gave support for the spilled oil investigation,but also served as an example for studying spilled oil accidents.展开更多
In this paper, gas chromatography-mass spectrometry （GS-MS） was used to build the standard fingerprint of volatile oil from Rosa multiflora Thunb. from 12 different habitats. Fourteen components in the volatile oil ...In this paper, gas chromatography-mass spectrometry （GS-MS） was used to build the standard fingerprint of volatile oil from Rosa multiflora Thunb. from 12 different habitats. Fourteen components in the volatile oil were identified as the indicator components ofR. multiflora, of which one was selected as the standard. The GC analysis conditions used for fingerprinting afford a very good separating effect. The similarity of the 12 volatile oils from R. multiflora Thunb. was more than 0.84, and the precision, stability and repeatability of the fingerprints were quite good. It could be concluded that the fingerprints can be used as the standard and as a quality control method for medicinal materials from R. multiflora Thunb..展开更多
目的:建立QuEChERS结合气相色谱-串联质谱法(Gas Chromatography-tandem Mass Spectrometry,GC-MS/MS)联合高效液相色谱-串联质谱法(High Performance Liquid Chromatography-tandem Mass Spectrometry,HPLC-MS/MS)检测大枣禁用农药残...目的:建立QuEChERS结合气相色谱-串联质谱法(Gas Chromatography-tandem Mass Spectrometry,GC-MS/MS)联合高效液相色谱-串联质谱法(High Performance Liquid Chromatography-tandem Mass Spectrometry,HPLC-MS/MS)检测大枣禁用农药残留的方法。方法:样品以体积比为1%的乙酸乙腈为溶剂,高速匀浆,采用QuEChERS法净化处理样品,分别以气质法和液质法进行分析,气质法采用外标法定量,液质法采用内标法定量。结果:气相色谱-串联质谱法检测的35种农药线性关系良好,相关系数均≥0.9972,定量限为0.0001~0.0055 mg·kg^(-1),回收率为65.54%~101.03%,相对标准偏差为0.38%~11.54%。高效液相色谱-串联质谱法测定的30种农药线性关系良好,相关系数均≥0.9896,定量限为0~0.00049mg·kg^(-1),回收率为62.92%~109.96%,相对标准偏差为0.04%~3.71%。结论:该方法操作简便、高效、定量定性准确、灵敏度高、重现性和稳定性良好,适用于大枣禁用农药残留的快速检测。展开更多
The chemical compositions of the essential oils obtained from six tree parts of Chrysophyllum albidum （Sapotaceae） were extracted by hydrodistillation and analyzed by gas chromatography （GC） and gas chromatography...The chemical compositions of the essential oils obtained from six tree parts of Chrysophyllum albidum （Sapotaceae） were extracted by hydrodistillation and analyzed by gas chromatography （GC） and gas chromatography-mass spectrometry （GC-MS）. A total of 65, 33, 45, 21, 25 and 18 compounds, representing 79.49%, 100%, 90.81%, 98.43%, 96.62% and 98.37% of the total oil, were identified in the fruit bark, root bark, stem bark, seed bark, leaf and seed, respectively. The dominant compounds in the essential oils in six tree parts were m-xylene （66.7%; seed）, p-xylene （21.4%; seed bark）, a-farnesene （38.1%; leaf）, hexadecanoic acid （14.7%; stem bark）, m-xylene （53.1%; root bark） and hexadecanoic acid （12.7%; fruit bark）. The essential oils were evaluated for their antibacterial, antioxidant and insecticidal activities using Alamar blue assay, DPPH radical scavenging activity and contact toxicity test, respectively. The oils displayed moderate antibacterial potentials to some tested organisms and low radical scavenging activity to DPPH. Rhyzopertha dominica was susceptible to C. albidum stem bark essential oil only.展开更多
Great variations have been found in composition and content of the essential oil of Zanthoxylum bungeanum Maxim. （Rutaceae）, resulting from vari- ous factors such as harvest time, drying and extraction methods （Hua...Great variations have been found in composition and content of the essential oil of Zanthoxylum bungeanum Maxim. （Rutaceae）, resulting from vari- ous factors such as harvest time, drying and extraction methods （Huang et al., 2006; Shao et al., 2013）, sol- vent and herbal parts used （Zhang, 1996; Cao and Zhang, 2010; Wang et al., 2011）. However, in terms of artificial introduction and cultivation, there is little research on the chemical composition of essential oil extracted from Z. bungeanum Maxim.展开更多
文摘In recent years,oil spill accidents occur frequently in the marine area of China.Finding out the spilled oil source is a key step in the relevant investigation.In this paper,a step-by-step fingerprinting identification method was used in a spilled oil accident in the Bohai Sea in 2002.Advanced chemical fingerprinting and data interpretation techniques were used to characterize the chemical composition and determine the possible sources of two spilled oil samples.The original gas chromatography -flame ionization detec-tion (GC-FID) chromatogram of saturated hydrocarbons was compared.The gas chromatography-mass spectrometry (GC/MS) chromatograms of aromatic hydrocarbons terpane and sterane,n-alkane and poly-aromatic hydrocarbons (PAHs) were analyzed.The correlation analysis on diagnostic ratios was performed with Student’s t-test.It is found that the oil fingerprinting of the spilled oil (designated as sz1) from the polluted sand beach was identical with the suspected oil (designated as ky1) from a nearby crude oil refinery factory.They both showed the fingerprinting character of mixed oil.The oil fingerprinting of the spilled oil (designated as ms1) collected from the port was significantly different from oil ky1 and oil sz1 and was with a lubricating oil fingerprint character.The identification result not only gave support for the spilled oil investigation,but also served as an example for studying spilled oil accidents.
文摘In this paper, gas chromatography-mass spectrometry （GS-MS） was used to build the standard fingerprint of volatile oil from Rosa multiflora Thunb. from 12 different habitats. Fourteen components in the volatile oil were identified as the indicator components ofR. multiflora, of which one was selected as the standard. The GC analysis conditions used for fingerprinting afford a very good separating effect. The similarity of the 12 volatile oils from R. multiflora Thunb. was more than 0.84, and the precision, stability and repeatability of the fingerprints were quite good. It could be concluded that the fingerprints can be used as the standard and as a quality control method for medicinal materials from R. multiflora Thunb..
文摘目的:建立QuEChERS结合气相色谱-串联质谱法(Gas Chromatography-tandem Mass Spectrometry,GC-MS/MS)联合高效液相色谱-串联质谱法(High Performance Liquid Chromatography-tandem Mass Spectrometry,HPLC-MS/MS)检测大枣禁用农药残留的方法。方法:样品以体积比为1%的乙酸乙腈为溶剂,高速匀浆,采用QuEChERS法净化处理样品,分别以气质法和液质法进行分析,气质法采用外标法定量,液质法采用内标法定量。结果:气相色谱-串联质谱法检测的35种农药线性关系良好,相关系数均≥0.9972,定量限为0.0001~0.0055 mg·kg^(-1),回收率为65.54%~101.03%,相对标准偏差为0.38%~11.54%。高效液相色谱-串联质谱法测定的30种农药线性关系良好,相关系数均≥0.9896,定量限为0~0.00049mg·kg^(-1),回收率为62.92%~109.96%,相对标准偏差为0.04%~3.71%。结论:该方法操作简便、高效、定量定性准确、灵敏度高、重现性和稳定性良好,适用于大枣禁用农药残留的快速检测。
文摘The chemical compositions of the essential oils obtained from six tree parts of Chrysophyllum albidum （Sapotaceae） were extracted by hydrodistillation and analyzed by gas chromatography （GC） and gas chromatography-mass spectrometry （GC-MS）. A total of 65, 33, 45, 21, 25 and 18 compounds, representing 79.49%, 100%, 90.81%, 98.43%, 96.62% and 98.37% of the total oil, were identified in the fruit bark, root bark, stem bark, seed bark, leaf and seed, respectively. The dominant compounds in the essential oils in six tree parts were m-xylene （66.7%; seed）, p-xylene （21.4%; seed bark）, a-farnesene （38.1%; leaf）, hexadecanoic acid （14.7%; stem bark）, m-xylene （53.1%; root bark） and hexadecanoic acid （12.7%; fruit bark）. The essential oils were evaluated for their antibacterial, antioxidant and insecticidal activities using Alamar blue assay, DPPH radical scavenging activity and contact toxicity test, respectively. The oils displayed moderate antibacterial potentials to some tested organisms and low radical scavenging activity to DPPH. Rhyzopertha dominica was susceptible to C. albidum stem bark essential oil only.
基金Project supported by the Scientific Research Project of Yangling in Shaanxi Province of China(No.2015NY-29)
文摘Great variations have been found in composition and content of the essential oil of Zanthoxylum bungeanum Maxim. （Rutaceae）, resulting from vari- ous factors such as harvest time, drying and extraction methods （Huang et al., 2006; Shao et al., 2013）, sol- vent and herbal parts used （Zhang, 1996; Cao and Zhang, 2010; Wang et al., 2011）. However, in terms of artificial introduction and cultivation, there is little research on the chemical composition of essential oil extracted from Z. bungeanum Maxim.
