[Objective] This study aimed to screen Chinese herbal medicines resistant to Chicken Escherichia coli and infectious laryngotracheitis virus. [Methed] Conven- tional punch method, test tube method and plate dilution m...[Objective] This study aimed to screen Chinese herbal medicines resistant to Chicken Escherichia coli and infectious laryngotracheitis virus. [Methed] Conven- tional punch method, test tube method and plate dilution method were adopted for in vitro susceptibility test of chicken E, coil strains O5 and O8 using 13 kinds of Chi- nese herbal medicines including Sanguisorba officinalis, Coptis chinensis, Anemar- rhena asphodeloides, Strobilanthes cusia, Agastache rugosa, etc.; chicken embryo inoculation experiment was adopted to screen Chinese herbal medicines resistant to chicken infectious laryngotracheitis virus. [Result] Sanguisorba officinalis, Fructus mume, Rheum officinale, Coptis chinensis, Herba Taraxaci, Anemarrhena asphode- Ioides, Scutellaria baicalensis and Rhizoma Fagopyri Cymosi had ideal antibacterial effect against chicken E. coil strain O5; Sanguisorba officinalis, Fructus mume, Rheum officinale, Coptis chinensis, Herba taraxaci and Rhizoma Fagopyri Cymosi had ideal antibacterial effect against chicken E. coil strain 08; other Chinese herbal medicines showed relatively poor or no antibacterial effect. Results of chicken embryo inoculation experiment showed that nine kinds of Chinese herbal medicines showed relatively strong anti-lLTV effect, including Forsythia suspensa, Radix Isatidis, Fofium isatidis, Flos Ionicerae, Radix codonopsis, Radix astragali, Atractylodes, Radix gly- cyrrhizae, and Pericarpium granati. [Conclusion] The study laid the foundation for fur- ther development of Chinese herbal compound preparations to treat chicken cofibacil- Iosis, infectious laryngotracheitis and other bacterial, viral diseases.展开更多
[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid contain...[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid containing ILTV into chickens to induce infectious laryngotracheitis, the clinical symptoms in infected chickens were observed; tissues of trachea, lungs, heart, liver, kidney and spleen were collected from dead chickens, and prepared into paraffin sections, followed by histopathological observation under a microscope. [Results] This disease occured in the inoculated chickens 3d later, with a morbidity rate of 95%. The main symptoms were dyspnea, asthma and coughing up of bloody exudate; yellowish-white pseudomembrane was observed on dissected larynx trachea; swelling, haemorrhage, and further erosion were observed on the in- fected tracheal mucosa, resulting in death of chickens, with the mortality rate of 25%; histopathological observation showed that parenchymal organs exhibited exuda- tive inflammation; swelling, degeneration, necrosis and shedding of epithelial cells were observed; disturbance of blood circulation occured.[Conclusion] This study pro- vides reference bases for the treatment of ILT.展开更多
For developing efficient vaccines, it is essential to identify which amino acid changes are most important to the survival of the virus. We investigate the amino acid substitution features in the Avian Infectious Bron...For developing efficient vaccines, it is essential to identify which amino acid changes are most important to the survival of the virus. We investigate the amino acid substitution features in the Avian Infectious Bronchitis Virus （AIBV） antigenic domain of a vaccine serotype （DE072） and a virulent viral strain （GA98） to better understand adaptive evolution of AIBV. In addition, the SARS Coronavirus （SARS-CoV） was also analyzed in the same way. It is interesting to find that extreme comparability exists between AIBV and SARS in amino acid substitution pattern. It suggests that amino acid changes that result in overall shift of residue charge and polarity should be paid special attention to during the development of vaccines.展开更多
A 53-year-old man with a history of blood transfusion at the age of 20 was admitted to our hospital because of liver dysfunction. He had bronchial asthma when he was 18 years old, which naturally resolved within 2 yea...A 53-year-old man with a history of blood transfusion at the age of 20 was admitted to our hospital because of liver dysfunction. He had bronchial asthma when he was 18 years old, which naturally resolved within 2 years. However, his bronchial asthma recurred at the age of 45 and was treated with oral theophylline. He was diagnosed as having chronic hepatitis C based on the histological and clinical findings, and then interferon (IFN) therapy was administered. The frequency of bronchial asthma attack was gradually decreasing after IFN therapy with marked improvement of hypereosinophilia. He achieved sustained viral response (SVR) and his bronchial asthma did not worsen even after the cessation of IFN. Hepatitis C virus (HCV) infection and IFN therapy were considered in the remission of asthma in this case. HCV infection could be the cause of bronchial asthma, especially in patients with late appearance of asthma.展开更多
[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected laye...[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.展开更多
Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG deriv...Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.展开更多
基金Supported by Project from Science Technology Department of Hebei Province(08820412D,1220408D,12820421D)Project from Science and Technology Bureau of Shijiazhuang(07150193A)PhD Fund of Hebei Normal University of Science and Technology(2007YB002)~~
文摘[Objective] This study aimed to screen Chinese herbal medicines resistant to Chicken Escherichia coli and infectious laryngotracheitis virus. [Methed] Conven- tional punch method, test tube method and plate dilution method were adopted for in vitro susceptibility test of chicken E, coil strains O5 and O8 using 13 kinds of Chi- nese herbal medicines including Sanguisorba officinalis, Coptis chinensis, Anemar- rhena asphodeloides, Strobilanthes cusia, Agastache rugosa, etc.; chicken embryo inoculation experiment was adopted to screen Chinese herbal medicines resistant to chicken infectious laryngotracheitis virus. [Result] Sanguisorba officinalis, Fructus mume, Rheum officinale, Coptis chinensis, Herba Taraxaci, Anemarrhena asphode- Ioides, Scutellaria baicalensis and Rhizoma Fagopyri Cymosi had ideal antibacterial effect against chicken E. coil strain O5; Sanguisorba officinalis, Fructus mume, Rheum officinale, Coptis chinensis, Herba taraxaci and Rhizoma Fagopyri Cymosi had ideal antibacterial effect against chicken E. coil strain 08; other Chinese herbal medicines showed relatively poor or no antibacterial effect. Results of chicken embryo inoculation experiment showed that nine kinds of Chinese herbal medicines showed relatively strong anti-lLTV effect, including Forsythia suspensa, Radix Isatidis, Fofium isatidis, Flos Ionicerae, Radix codonopsis, Radix astragali, Atractylodes, Radix gly- cyrrhizae, and Pericarpium granati. [Conclusion] The study laid the foundation for fur- ther development of Chinese herbal compound preparations to treat chicken cofibacil- Iosis, infectious laryngotracheitis and other bacterial, viral diseases.
基金Supported by the Special Project of Department of Science and Technology of Hebei Province(08820412D)the Special Project of Qinhuangdao Municipal Bureau of Science and Technology[Qinkeji(2003)30-35]+1 种基金the Special Project of Shijiazhuang Municipal Bureau of Science and Technology(07150193A)the Scientific Research Innovation Team Project of Hebei Normal University of Science and Technology(TD201201)~~
文摘[Objective] This study aimed to investigate the histopathological changes in chickens artificially infected with infectious laryngotracheitis（ILT）. [Method] Through ar- tificial injection of allantoic liquid containing ILTV into chickens to induce infectious laryngotracheitis, the clinical symptoms in infected chickens were observed; tissues of trachea, lungs, heart, liver, kidney and spleen were collected from dead chickens, and prepared into paraffin sections, followed by histopathological observation under a microscope. [Results] This disease occured in the inoculated chickens 3d later, with a morbidity rate of 95%. The main symptoms were dyspnea, asthma and coughing up of bloody exudate; yellowish-white pseudomembrane was observed on dissected larynx trachea; swelling, haemorrhage, and further erosion were observed on the in- fected tracheal mucosa, resulting in death of chickens, with the mortality rate of 25%; histopathological observation showed that parenchymal organs exhibited exuda- tive inflammation; swelling, degeneration, necrosis and shedding of epithelial cells were observed; disturbance of blood circulation occured.[Conclusion] This study pro- vides reference bases for the treatment of ILT.
文摘For developing efficient vaccines, it is essential to identify which amino acid changes are most important to the survival of the virus. We investigate the amino acid substitution features in the Avian Infectious Bronchitis Virus （AIBV） antigenic domain of a vaccine serotype （DE072） and a virulent viral strain （GA98） to better understand adaptive evolution of AIBV. In addition, the SARS Coronavirus （SARS-CoV） was also analyzed in the same way. It is interesting to find that extreme comparability exists between AIBV and SARS in amino acid substitution pattern. It suggests that amino acid changes that result in overall shift of residue charge and polarity should be paid special attention to during the development of vaccines.
文摘A 53-year-old man with a history of blood transfusion at the age of 20 was admitted to our hospital because of liver dysfunction. He had bronchial asthma when he was 18 years old, which naturally resolved within 2 years. However, his bronchial asthma recurred at the age of 45 and was treated with oral theophylline. He was diagnosed as having chronic hepatitis C based on the histological and clinical findings, and then interferon (IFN) therapy was administered. The frequency of bronchial asthma attack was gradually decreasing after IFN therapy with marked improvement of hypereosinophilia. He achieved sustained viral response (SVR) and his bronchial asthma did not worsen even after the cessation of IFN. Hepatitis C virus (HCV) infection and IFN therapy were considered in the remission of asthma in this case. HCV infection could be the cause of bronchial asthma, especially in patients with late appearance of asthma.
文摘[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.
文摘Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.
