To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out ...To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance determining region) gyrA, gyrB, parC and parE were amplified by PCR Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed Results Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum展开更多
基金Supported by the Food Safety Key Technology Project in the National Science and Technology Pillar Program during the 11th Five-Year Plan Period(2006 BAK02A03)Anhui University Provincial Natural Science Research Project(KJ2007B294)
基金ThisstudywassupportedbyagrantfromtheEducationCommitteeofHunanProvince (No 0 0A0 0 9)
文摘To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance determining region) gyrA, gyrB, parC and parE were amplified by PCR Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed Results Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum
