复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果研究

目的验证复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果。方法选用琼脂扩散法,比较复方氟康唑凝胶与氟康唑凝胶对耐氟康唑假丝酵母菌的体外抑菌效果;通过感染耐氟康唑假丝酵母菌大鼠治疗试验,比较复方氟康唑凝胶与氟康唑凝胶治疗效果。结果复方氟康唑凝胶抑菌圈直径(18.55±0.03) mm (n=20),氟康唑凝胶抑菌圈直径(7.12±0.01) mm (n=20),差异具有统计学意义(P<0.05);复方氟康唑凝胶治疗感染耐氟康唑假丝酵母菌大鼠15 d的转阴率为80%,氟康唑凝胶为40%,差异具有统计学意义(P<0.05,n=15);复方氟康唑凝胶抑制阴道灌洗液炎性细胞因子水平能力、减轻病变损伤程度明显高于氟康唑凝胶(P<0.05,n=15)。结论复方氟康唑凝胶对耐氟康唑假丝酵母菌的抗菌效果肯定,较氟康唑凝胶具有优势。

氟康唑凝胶的研制及临床应用

目的:研制氟康唑凝胶,并制定质量标准。方法:采用一阶导数分光光度法进行含量测定。结果:氟康唑平均回收 率为(101.7±0.65)％,RSD为0.7％。结论:氟康唑凝胶制备工艺简单,含量测定简便,值得推广应用。

均匀设计优化复方氟康唑凝胶基质处方

目的利用均匀设计法优化复方氟康唑凝胶基质处方。方法采用4因素15水平均匀设计方案,以复方氟康唑凝胶的外观性状、粒度、p H及酸碱缓冲能力、黏度为综合评价指标,对基质处方进行优化。结果优化基质处方组成为:每100 g复方氟康唑凝胶含卡波姆-940 0.9 g,磷酸盐缓冲液(p H 7.8)10.0 g,三乙醇胺1.9 g,乙醇19.0 g。结论按优化处方制备的复方氟康唑凝胶具有外观细腻、色泽均匀、药物稳定,适用性好的特点。

高效液相法在氟康唑温敏型眼用凝胶中氟康唑含量测定中的应用

目的建立高效液相法测定氟康唑温敏型眼用凝胶中氟康唑的含量。方法采用C 18色谱柱,流动相为乙腈-0.063%甲酸铵溶液(20∶80),检测波长260 nm。结果氟康唑温敏型眼用凝胶中氟康唑在该色谱条件下能够有效分离,氟康唑含量测定浓度在5.9~239.8μg·mL-1范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为98.96%,RSD为1.29%。结论该方法简便、准确、稳定,可用于氟康唑温敏型眼用凝胶中氟康唑含量测定。

苦参凝胶与氟康唑胶囊结合在霉菌性阴道炎中的效果研究

目的观察对霉菌性阴道炎患者采用苦参凝胶与氟康唑胶囊结合治疗的效果。方法选取2015年3月～2017年8月收治的霉菌性阴道炎患者140例进行作为研究对象,采用随机数表法将其分成两组,对照组患者70例采用氟康唑胶囊治疗,实验组患者70例,采用苦参凝胶与氟康唑胶囊结合治疗。比较两组患者的治疗效果。结果实验组的治疗总有效率高于对照组,不良症状消失时间低于对照组,P<0.05。结论对霉菌性阴道炎患者采用苦参凝胶与氟康唑胶囊结合治疗,不仅可以改善该疾病的反复发作,同时还可以改善该疾病的不良症状,具有推广应用价值。

氟康唑阴道凝胶的制备及质量控制

目的 研制氟康唑阴道凝胶并制定质量标准。方法 用HPLC测定氟康唑凝胶中氟康唑的含量，进行稳定性试验。结果 处方筛选合理，质量稳定，方法平均回收率99．6％。结论 氟康唑凝胶制各工艺完善，含量测定方法简便，可推广应用。

Preparation of liposomal fluconazole gel and in vitro transdermal delivery

Aim Liposomal fluconazole gel was prepared and its properties were studied. Methods The fluconazole liposomes were prepared by film dispersion method. Their shapes and sizes were observed by transmission electronic microscope and particle size analyzer, respectively. The skin permeation of liposomal gel was studied on rat skin by permeation cell. Results The entrapment efficiency of flueonazole liposomes was 47.68%. The fluconazole liposomes were oval or round in shape, and their average diameter was 250 ± 8 nm. The accumulative skin permeation of liposomal fluconazole gel （25.27%） was lower than that of non-liposomal fluconazole gel （36.72%）, but fluconazole retained in rat skin of liposomal gel （162 ± 15 μg·cm^-2） was higher than that of nonliposomal gel （48 ± 6μg·cm^-2）. Conclusion Liposomal fluconazole gel can significantly increase the deposited amounts of fluconazole in rat skin and it may be beneficial for topical use.