Aim Liposomal fluconazole gel was prepared and its properties were studied. Methods The fluconazole liposomes were prepared by film dispersion method. Their shapes and sizes were observed by transmission electronic mi...Aim Liposomal fluconazole gel was prepared and its properties were studied. Methods The fluconazole liposomes were prepared by film dispersion method. Their shapes and sizes were observed by transmission electronic microscope and particle size analyzer, respectively. The skin permeation of liposomal gel was studied on rat skin by permeation cell. Results The entrapment efficiency of flueonazole liposomes was 47.68%. The fluconazole liposomes were oval or round in shape, and their average diameter was 250 ± 8 nm. The accumulative skin permeation of liposomal fluconazole gel （25.27%） was lower than that of non-liposomal fluconazole gel （36.72%）, but fluconazole retained in rat skin of liposomal gel （162 ± 15 μg·cm^-2） was higher than that of nonliposomal gel （48 ± 6μg·cm^-2）. Conclusion Liposomal fluconazole gel can significantly increase the deposited amounts of fluconazole in rat skin and it may be beneficial for topical use.展开更多
AIM:To study the anti-hepatocarcinoma effects of 5-fluorouracil (5-Fu) encapsulated by galactosylceramide liposomes (5-Fu-GCL) in vivo and in vitro. METHODS: Tumor-bearing animal model and HepA cell line were respecti...AIM:To study the anti-hepatocarcinoma effects of 5-fluorouracil (5-Fu) encapsulated by galactosylceramide liposomes (5-Fu-GCL) in vivo and in vitro. METHODS: Tumor-bearing animal model and HepA cell line were respectively adopted to evaluate the anti-tumor effects of 5-Fu-GCL in vivo and in vitro. Tumor cell growth inhibition effects of 5-Fu-GCL in vitro were assessed by cell viability assay and MTT assay. In vivo experiment, the inhibitory effects on tumor growth were evaluated by tumor inhibition rate and animal survival days. High performance liquid chromatography was used to detect the concentration-time course of 5-Fu-GCL in intracellular fluid in vitro and the distribution of 5-Fu-GCL in liver tumor tissues in vivo. Apoptosis and cell cycle of tumor cells were demonstrated by flow cytometry. RESULTS: In vitro experiment, 5-Fu-GCL (6.25-100 μmol/L) and free 5-Fu significantly inhibited HepA cell growth. Furthermore, IC50 of 5-Fu-GCL (34.5 μmol/L) was lower than that of free 5-Fu (51.2 μmol/L). In vivo experiment, 5-Fu-GCL (20, 40, 80 mg/kg) significantly suppressed the tumor growth in HepA bearing mice model. Compared with free 5-Fu, the area under curve of 5-Fu-GCL in intracellular fluid increased 2.6 times. Similarly, the distribution of 5-Fu-GCL in liver tumor tissues was significantly higher than that of free 5-Fu. After being treated with 5-Fu-GCL, the apoptotic rate and the proportion of HepA cells in the S phase increased, while the proportion in the G0/G1 and G2/M phases decreased. CONCLUSION: 5-Fu-GCL appears to have anti-hepatocarcinoma effects and its drug action is better than free 5-Fu. Its mechanism is partly related to increased drug concentrations in intracellular fluid and liver tumor tissues, enhanced tumor cell apoptotic rate and arrest of cell cycle in S phase.展开更多
Objective:To investigate the anticancer effects of Xi Huang Capsule(XH)in vivo,thirty-two Nu/Nu mice inoculated with human breast cancer SKBR-3 cells and twenty-four Nu/Nu mice inoculated with murine breast cancer 4T1...Objective:To investigate the anticancer effects of Xi Huang Capsule(XH)in vivo,thirty-two Nu/Nu mice inoculated with human breast cancer SKBR-3 cells and twenty-four Nu/Nu mice inoculated with murine breast cancer 4T1 cells were randomized into the control group,XH group,5-FU group and combination of XH and 5-FU group in a 1:1:1:1 ratio.Method:The 5-FU group was injected with 5-FU at 30 mg/kg intraperitoneally every third day;XH group received doses of 0.25g/kg of XH by gastric perfusion each day;The control group was injected daily with normal saline(N.S.)intraperitoneally and the combination group was treated with 5-FU and XH on the same schedules as above.All treatments lasted for 15 days in human SKBR-3 breast cancer cells and 11 days in mice breast cancer 4T1 cells.Tumor volume,tumor weight,organ index,and change in body weight of nude mice were measured,respectively.PCNA and vimentin protein expression were examined by immunohistochemical assay.Results:In SKBR-3 cell xenograft tumor experiments,the XH group,5-FU group and the combination group had significantly smaller tumor volumes(966.39±80.23mm3,892.21±150.77mm3,817.93±162.47 mm3,respectively),and lower tumor weights(0.90±0.14g,0.84±0.32,0.86±0.24g,respectively),as compared with the control group(all P<0.05).The combination group had the highest tumor inhibition rate(38.7%).The similar results emerged in 4T1 cell xenograft tumor.Only the combination group had the least body weight increase of SKBR-3 cells xenograft tumor(P<0.05 as compared with the control group).In SKBR-3 cell xenograft tumor experiments,the 5-FU group had a lower Liver index(43.02±5.00mg/g versus 50.95±4.59mg/g)as compared with the control group(P<0.05),whereas the combination group reversed the changes in the 5-FU group with Liver index of 49.69±4.81 mg/g(P<0.05).The combination group had a higher Spleen index(5.95±1.62 versus 4.72±0.66mg/g)as compared with the control group,and had a higher Spleen index as compared with the 5-FU group(4.54±0.79 mg/g,P<0.05).In 4T1 cell xenograft tumor experiments,the 5-FU group and the combination group had a lower Liver index(47.69±6.41,49.87±5.96 versus 58.95±7.33),but Liver index of XH group had no significantly difference as compared with the control group.The Spleen index was the same to that in SKBR-3 cells xenograft tumor.PCNA and vimentin expression of XH group was significantly lower than that of the control group.Conclusion:The treatment of XH was equally effective in the inhibition of tumor growth,and may have potentially additional benefit in improving the general condition and immunity of the mice not only in human breast cancer cell but also in rat mammary carcinoma in vivo.展开更多
Lanthanum alginate bead is a new, highly active adsorbent. In the present study, we investigated its ad- sorption performance and its adsorption mechanism. The adsorption isotherm for fluoride onto lanthanum alginate ...Lanthanum alginate bead is a new, highly active adsorbent. In the present study, we investigated its ad- sorption performance and its adsorption mechanism. The adsorption isotherm for fluoride onto lanthanum alginate b ead fits the Langmuir model well, and the maximum adsorption capacity is 197.2 mg·g-1. X-ray diffraction shows the amorphous nature of lanthanum alginate bead, which allows for better accessibility to fluoride and thus better activity. Infrared spectra of lanthanum alginate bead before and after adsorption confirm its stable skeletal structure. Scanning electron microscopy shows that the dense surface structure of the adsorbent appear cracks after adsorption. T he adsorption mechanism of lanthanum alginate bead is considered as an ion exchange between F- and Cl- or OH-, as verified from the adsorbent and the solution by pH effect, energy dispersive X-ray, and ion chromatography.展开更多
文摘Aim Liposomal fluconazole gel was prepared and its properties were studied. Methods The fluconazole liposomes were prepared by film dispersion method. Their shapes and sizes were observed by transmission electronic microscope and particle size analyzer, respectively. The skin permeation of liposomal gel was studied on rat skin by permeation cell. Results The entrapment efficiency of flueonazole liposomes was 47.68%. The fluconazole liposomes were oval or round in shape, and their average diameter was 250 ± 8 nm. The accumulative skin permeation of liposomal fluconazole gel （25.27%） was lower than that of non-liposomal fluconazole gel （36.72%）, but fluconazole retained in rat skin of liposomal gel （162 ± 15 μg·cm^-2） was higher than that of nonliposomal gel （48 ± 6μg·cm^-2）. Conclusion Liposomal fluconazole gel can significantly increase the deposited amounts of fluconazole in rat skin and it may be beneficial for topical use.
基金Supported by the Key Teacher Foundation of Ministry of Education of China, No. 1869 Young Teacher Foundation of Department of Education of Anhui Province, No. 2000jp112
文摘AIM:To study the anti-hepatocarcinoma effects of 5-fluorouracil (5-Fu) encapsulated by galactosylceramide liposomes (5-Fu-GCL) in vivo and in vitro. METHODS: Tumor-bearing animal model and HepA cell line were respectively adopted to evaluate the anti-tumor effects of 5-Fu-GCL in vivo and in vitro. Tumor cell growth inhibition effects of 5-Fu-GCL in vitro were assessed by cell viability assay and MTT assay. In vivo experiment, the inhibitory effects on tumor growth were evaluated by tumor inhibition rate and animal survival days. High performance liquid chromatography was used to detect the concentration-time course of 5-Fu-GCL in intracellular fluid in vitro and the distribution of 5-Fu-GCL in liver tumor tissues in vivo. Apoptosis and cell cycle of tumor cells were demonstrated by flow cytometry. RESULTS: In vitro experiment, 5-Fu-GCL (6.25-100 μmol/L) and free 5-Fu significantly inhibited HepA cell growth. Furthermore, IC50 of 5-Fu-GCL (34.5 μmol/L) was lower than that of free 5-Fu (51.2 μmol/L). In vivo experiment, 5-Fu-GCL (20, 40, 80 mg/kg) significantly suppressed the tumor growth in HepA bearing mice model. Compared with free 5-Fu, the area under curve of 5-Fu-GCL in intracellular fluid increased 2.6 times. Similarly, the distribution of 5-Fu-GCL in liver tumor tissues was significantly higher than that of free 5-Fu. After being treated with 5-Fu-GCL, the apoptotic rate and the proportion of HepA cells in the S phase increased, while the proportion in the G0/G1 and G2/M phases decreased. CONCLUSION: 5-Fu-GCL appears to have anti-hepatocarcinoma effects and its drug action is better than free 5-Fu. Its mechanism is partly related to increased drug concentrations in intracellular fluid and liver tumor tissues, enhanced tumor cell apoptotic rate and arrest of cell cycle in S phase.
文摘Objective:To investigate the anticancer effects of Xi Huang Capsule(XH)in vivo,thirty-two Nu/Nu mice inoculated with human breast cancer SKBR-3 cells and twenty-four Nu/Nu mice inoculated with murine breast cancer 4T1 cells were randomized into the control group,XH group,5-FU group and combination of XH and 5-FU group in a 1:1:1:1 ratio.Method:The 5-FU group was injected with 5-FU at 30 mg/kg intraperitoneally every third day;XH group received doses of 0.25g/kg of XH by gastric perfusion each day;The control group was injected daily with normal saline(N.S.)intraperitoneally and the combination group was treated with 5-FU and XH on the same schedules as above.All treatments lasted for 15 days in human SKBR-3 breast cancer cells and 11 days in mice breast cancer 4T1 cells.Tumor volume,tumor weight,organ index,and change in body weight of nude mice were measured,respectively.PCNA and vimentin protein expression were examined by immunohistochemical assay.Results:In SKBR-3 cell xenograft tumor experiments,the XH group,5-FU group and the combination group had significantly smaller tumor volumes(966.39±80.23mm3,892.21±150.77mm3,817.93±162.47 mm3,respectively),and lower tumor weights(0.90±0.14g,0.84±0.32,0.86±0.24g,respectively),as compared with the control group(all P<0.05).The combination group had the highest tumor inhibition rate(38.7%).The similar results emerged in 4T1 cell xenograft tumor.Only the combination group had the least body weight increase of SKBR-3 cells xenograft tumor(P<0.05 as compared with the control group).In SKBR-3 cell xenograft tumor experiments,the 5-FU group had a lower Liver index(43.02±5.00mg/g versus 50.95±4.59mg/g)as compared with the control group(P<0.05),whereas the combination group reversed the changes in the 5-FU group with Liver index of 49.69±4.81 mg/g(P<0.05).The combination group had a higher Spleen index(5.95±1.62 versus 4.72±0.66mg/g)as compared with the control group,and had a higher Spleen index as compared with the 5-FU group(4.54±0.79 mg/g,P<0.05).In 4T1 cell xenograft tumor experiments,the 5-FU group and the combination group had a lower Liver index(47.69±6.41,49.87±5.96 versus 58.95±7.33),but Liver index of XH group had no significantly difference as compared with the control group.The Spleen index was the same to that in SKBR-3 cells xenograft tumor.PCNA and vimentin expression of XH group was significantly lower than that of the control group.Conclusion:The treatment of XH was equally effective in the inhibition of tumor growth,and may have potentially additional benefit in improving the general condition and immunity of the mice not only in human breast cancer cell but also in rat mammary carcinoma in vivo.
基金Supported by the Major National Science and Technology Special Project on Treatment and Control of Water Pollution(2009ZX07425-006)State Key Laboratory of Environmental Simulation and Pollution Control (09K04ESPCT)
文摘Lanthanum alginate bead is a new, highly active adsorbent. In the present study, we investigated its ad- sorption performance and its adsorption mechanism. The adsorption isotherm for fluoride onto lanthanum alginate b ead fits the Langmuir model well, and the maximum adsorption capacity is 197.2 mg·g-1. X-ray diffraction shows the amorphous nature of lanthanum alginate bead, which allows for better accessibility to fluoride and thus better activity. Infrared spectra of lanthanum alginate bead before and after adsorption confirm its stable skeletal structure. Scanning electron microscopy shows that the dense surface structure of the adsorbent appear cracks after adsorption. T he adsorption mechanism of lanthanum alginate bead is considered as an ion exchange between F- and Cl- or OH-, as verified from the adsorbent and the solution by pH effect, energy dispersive X-ray, and ion chromatography.
