AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control...AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG)model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24J/cm2doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter,the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope.RESULTS: In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm2 dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P＜0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P＜0.01), the gastric mucosal inflammation cells were decreased (P＜0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal.CONCLUSION: 3.36J/cm2 dose of He-Ne laser has a significant effect on CAG in rats.展开更多
The frequency of digitally controlled iodine stabilized He-Ne laser locked to a hyperfine component in ^127I2 was measured using the National Metrology Institute of Japan＇s reference iodine stabilized He-Ne laser. Th...The frequency of digitally controlled iodine stabilized He-Ne laser locked to a hyperfine component in ^127I2 was measured using the National Metrology Institute of Japan＇s reference iodine stabilized He-Ne laser. This laser is operated under the conditions of the practical implementation and its frequency with respect to the International Committee for Weights and Measure recommended value is known from international comparisons. Adopting a sampling rate of 120 kHz for the control system of an iodine stabilized He-Ne laser enables frequency deviation of the test laser from the reference laser by ＋ 5 kHz （relative accuracy of 1 ×10^11） thus limited only by the reproducibility of the iodine stabilized He-Ne laser itself.展开更多
The paper presents a study on the effects of low intensity laser irradiation on morphological changes in plants sprouted from maize hybrid seeds （two hybrids） and wheat seeds. Pre-sowing laser irradiation treatment ...The paper presents a study on the effects of low intensity laser irradiation on morphological changes in plants sprouted from maize hybrid seeds （two hybrids） and wheat seeds. Pre-sowing laser irradiation treatment on the seeds was done, intervals from 10 s to 15 min （approximately）, by using a diode laser output power of 12 mW at 904 nm wavelength or with He-Ne laser with output power of 50 mW and 632.8 nm wavelength. Before irradiation seeds were divided into groups （wet and dry, and then in subgroups-irradiated or control groups）. We used maize hybrids, Amilacea and Identata and wheat （Triticum aestivum）. The reflection coefficient in visible range was done for maize varieties. Obtained data show the influence of laser beam to better plant growth. Better results are obtained for dry seed irradiation than for wet. In order to investigate the effect of laser beam and in general to clarify a lot of unsolved photo processes related to bioorganisms at macroscopic and microscopic levels, some optical constants of selected plant families were researched. At the same time, the influence of laser beams of common wavelengths to the selected plants was monitored. Morphological processes of plants （seeds and leaves） irradiated under different conditions and plant growing dynamics were contemplated. The definite correlation analyses of obtained results were made, clearly speaking about the influence of small-dose radiation to characteristics （quantitative and other genetic, bio-stimulating effects） of future plant growth.展开更多
To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulato...To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulators on the formation of atrophic gastritis, to study the expressions of EGF and EGFR in rats with chronic atrophic gastritis (CAG) after the irradiation of He-Ne laser, and to investigate the relations of He-Ne laser and precancerous lesion or apoptosis. The rats were divided into normal group, model group and laser group. The models of CAG rats were established with enema which was the mixed liquor consisted of sodium salicylate and alcohol, combined with irregular fasting and compulsive sporting as pathogenic factors. He-Ne laser(3.36 J/cm2 ) was used to irradiate CAG rats, once a day for 7 min, 20 days as a course of treatment; the expressions of EGF and EGFR were detected with immunohistochemical method. During the process of antral atrophy, the expressions of EGF and EGFR increased, they were higher in model group than those in normal group (P<0.05). After the irradiation of He-Ne laser, the expressions of EGF and EGFR were obviously lower than those in model group (P<0.05). We draw the conclusion as follows: gastric mucosa of model rat is in a hyper-proliferation status, with high protein expressions of apoptosis suppressor EGF and EGFR. CAG has some correlation with the imbalance between cell proliferation and apoptosis. He-Ne laser (3.36 J/cm2 ) can reduce the expressions of EGF and EGFR, which is an effective physiostimulator to stimulate the gastric mucosa of rat. The appropriate secretions of EGF and EGFR are propitious to the repair and regeneration for the gastric mucosa tissues, thus preventing CAG to canceration.展开更多
Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of ...Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.展开更多
Objective： To observe the clinical effects of the unilateral nervous tinnitus treated by He-Ne laser acupuncture.Methods： A total of 30 patients of unilateral nervous tinnitus were treated with He-Ne laser acupunctu...Objective： To observe the clinical effects of the unilateral nervous tinnitus treated by He-Ne laser acupuncture.Methods： A total of 30 patients of unilateral nervous tinnitus were treated with He-Ne laser acupuncture.The disposable filiform needles of 0.40 mm × 25 mm were used. Ermén（耳门 TE 21）, Tīnggōng（听宫 SI 19）,Tinghui（听会 GB 2）, Yìfēng（翳风 TE 17）,Shuàigǔ（率谷 GB 8） and Zhōngzhǔ（中渚 TE 3） were adopted on the affected side. The input and output ends of the fiber-optic catheter of the laser instrument were connected at a pair of acupoints（TE 21 connect to SI 19, GB 2 connect to TE 17, GB 8 connect to TE 3）.Each pair of acupoints was stimulated with He-Ne laser irradiation for 10 min, 8-10 mW in the output power and 6328 A in the wavelength. The needles were retained for 30 min totally. The treatment was given once a day, 5 times a week, continuously for 2 weeks.Results： Of 30 cases, 19 cases were cured clinically, accounting for 63.3%（19/30）; 7 cases effective remarkably, accounting for 23.3%（7/30） and 4 cases failed, accounting for 13.3%（4/30）.Conclusion： He-Ne laser acupuncture achieves the satisfactory effects on treating nervous tinnitus and it is easily accepted by the patients. Hence, this therapy deserves to be promoted in clinical application.展开更多
基金Supported by the Natural Science Foundation of Hebei Province, No. 301427
文摘AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG)model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24J/cm2doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter,the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope.RESULTS: In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm2 dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P＜0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P＜0.01), the gastric mucosal inflammation cells were decreased (P＜0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal.CONCLUSION: 3.36J/cm2 dose of He-Ne laser has a significant effect on CAG in rats.
文摘The frequency of digitally controlled iodine stabilized He-Ne laser locked to a hyperfine component in ^127I2 was measured using the National Metrology Institute of Japan＇s reference iodine stabilized He-Ne laser. This laser is operated under the conditions of the practical implementation and its frequency with respect to the International Committee for Weights and Measure recommended value is known from international comparisons. Adopting a sampling rate of 120 kHz for the control system of an iodine stabilized He-Ne laser enables frequency deviation of the test laser from the reference laser by ＋ 5 kHz （relative accuracy of 1 ×10^11） thus limited only by the reproducibility of the iodine stabilized He-Ne laser itself.
文摘The paper presents a study on the effects of low intensity laser irradiation on morphological changes in plants sprouted from maize hybrid seeds （two hybrids） and wheat seeds. Pre-sowing laser irradiation treatment on the seeds was done, intervals from 10 s to 15 min （approximately）, by using a diode laser output power of 12 mW at 904 nm wavelength or with He-Ne laser with output power of 50 mW and 632.8 nm wavelength. Before irradiation seeds were divided into groups （wet and dry, and then in subgroups-irradiated or control groups）. We used maize hybrids, Amilacea and Identata and wheat （Triticum aestivum）. The reflection coefficient in visible range was done for maize varieties. Obtained data show the influence of laser beam to better plant growth. Better results are obtained for dry seed irradiation than for wet. In order to investigate the effect of laser beam and in general to clarify a lot of unsolved photo processes related to bioorganisms at macroscopic and microscopic levels, some optical constants of selected plant families were researched. At the same time, the influence of laser beams of common wavelengths to the selected plants was monitored. Morphological processes of plants （seeds and leaves） irradiated under different conditions and plant growing dynamics were contemplated. The definite correlation analyses of obtained results were made, clearly speaking about the influence of small-dose radiation to characteristics （quantitative and other genetic, bio-stimulating effects） of future plant growth.
文摘To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulators on the formation of atrophic gastritis, to study the expressions of EGF and EGFR in rats with chronic atrophic gastritis (CAG) after the irradiation of He-Ne laser, and to investigate the relations of He-Ne laser and precancerous lesion or apoptosis. The rats were divided into normal group, model group and laser group. The models of CAG rats were established with enema which was the mixed liquor consisted of sodium salicylate and alcohol, combined with irregular fasting and compulsive sporting as pathogenic factors. He-Ne laser(3.36 J/cm2 ) was used to irradiate CAG rats, once a day for 7 min, 20 days as a course of treatment; the expressions of EGF and EGFR were detected with immunohistochemical method. During the process of antral atrophy, the expressions of EGF and EGFR increased, they were higher in model group than those in normal group (P<0.05). After the irradiation of He-Ne laser, the expressions of EGF and EGFR were obviously lower than those in model group (P<0.05). We draw the conclusion as follows: gastric mucosa of model rat is in a hyper-proliferation status, with high protein expressions of apoptosis suppressor EGF and EGFR. CAG has some correlation with the imbalance between cell proliferation and apoptosis. He-Ne laser (3.36 J/cm2 ) can reduce the expressions of EGF and EGFR, which is an effective physiostimulator to stimulate the gastric mucosa of rat. The appropriate secretions of EGF and EGFR are propitious to the repair and regeneration for the gastric mucosa tissues, thus preventing CAG to canceration.
文摘Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.
基金Supported by Weihai Technological Development Program:No.2102016Y0101~~
文摘Objective： To observe the clinical effects of the unilateral nervous tinnitus treated by He-Ne laser acupuncture.Methods： A total of 30 patients of unilateral nervous tinnitus were treated with He-Ne laser acupuncture.The disposable filiform needles of 0.40 mm × 25 mm were used. Ermén（耳门 TE 21）, Tīnggōng（听宫 SI 19）,Tinghui（听会 GB 2）, Yìfēng（翳风 TE 17）,Shuàigǔ（率谷 GB 8） and Zhōngzhǔ（中渚 TE 3） were adopted on the affected side. The input and output ends of the fiber-optic catheter of the laser instrument were connected at a pair of acupoints（TE 21 connect to SI 19, GB 2 connect to TE 17, GB 8 connect to TE 3）.Each pair of acupoints was stimulated with He-Ne laser irradiation for 10 min, 8-10 mW in the output power and 6328 A in the wavelength. The needles were retained for 30 min totally. The treatment was given once a day, 5 times a week, continuously for 2 weeks.Results： Of 30 cases, 19 cases were cured clinically, accounting for 63.3%（19/30）; 7 cases effective remarkably, accounting for 23.3%（7/30） and 4 cases failed, accounting for 13.3%（4/30）.Conclusion： He-Ne laser acupuncture achieves the satisfactory effects on treating nervous tinnitus and it is easily accepted by the patients. Hence, this therapy deserves to be promoted in clinical application.
