Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Diseas...Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Disease, decreased activity of thioredoxin coexists with increased level of adiponectin as partial pressure of arterial oxygen decreases. From the results with adiponectin-knockout mice and in vitro experiments, we have found a reciprocal inhibitory relationship, by which adiponectin inhibits cytosolic thioredoxin （Trxl） expression, whereas serum Trxl influences adiponectin multimerization. The associa- tion between extracellular Trxl and adiponectin attenuates their functions. This relationship is dynamic, and correlated with a body＇s physiological conditions.展开更多
Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of th...Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.展开更多
基金Acknowledgments This work was supported by the National Natural Science Foundation of China （31170764）, the Main Direction Pro- gram of Knowledge Innovation of Chinese Academy of Sciences （KSCX2-EW-J-29）, and Science and Education Integration Project of University of Chinese Academy of Sciences （KJRH2015-008）.
文摘Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Disease, decreased activity of thioredoxin coexists with increased level of adiponectin as partial pressure of arterial oxygen decreases. From the results with adiponectin-knockout mice and in vitro experiments, we have found a reciprocal inhibitory relationship, by which adiponectin inhibits cytosolic thioredoxin （Trxl） expression, whereas serum Trxl influences adiponectin multimerization. The associa- tion between extracellular Trxl and adiponectin attenuates their functions. This relationship is dynamic, and correlated with a body＇s physiological conditions.
基金supported by the National Basic Research Program of China (2012CB721103)the National Natural Science Foundation of China (21102143)
文摘Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.
