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氨基酸营养对At.f菌生长迟缓期的影响
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作者 张一 《安徽农业科学》 CAS 北大核心 2011年第25期15195-15197,共3页
[目的]研究氨基酸营养对At.f菌生长迟缓期的影响。[方法]将At.f在分别加入20种氨基酸的培养基中培养,利用重铬酸钾滴定法测定各时段Fe2+利用情况,并检测添加氨基酸后培养基pH的变化。[结果]非极性氨基酸对缩短At.f菌的迟缓期的效果较明... [目的]研究氨基酸营养对At.f菌生长迟缓期的影响。[方法]将At.f在分别加入20种氨基酸的培养基中培养,利用重铬酸钾滴定法测定各时段Fe2+利用情况,并检测添加氨基酸后培养基pH的变化。[结果]非极性氨基酸对缩短At.f菌的迟缓期的效果较明显;苯丙氨酸、酪氨酸、脯氨酸、色氨酸四种成环氨基酸有较好的效果;丝氨酸、谷氨酰胺、组氨酸有明显的抑制作用。[结论]非极性氨基酸能有效缩短At.f菌生长迟缓期,部分极性氨基酸则起到相反效果。 展开更多
关键词 氧化亚铁硫感菌 迟缓期 氨基酸 重铬酸钾滴定法 PH
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Characterization of acylated homoserine lactone derivatives and their influence on biofilms of Acidithiobacillus ferrooxidans BY-3 under arsenic stress 被引量:1
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作者 GAO Qi-yu TANG De-ping +2 位作者 SONG Peng ZHOU Jian-ping LI Hong-yu 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第1期52-63,共12页
Quorum sensing is one kind of cell-to-cell signalling system among microorganisms that works in response to their population density via autoinducers exemplified by AHL and oligopeptides. In this study, fourteen AHL d... Quorum sensing is one kind of cell-to-cell signalling system among microorganisms that works in response to their population density via autoinducers exemplified by AHL and oligopeptides. In this study, fourteen AHL derivatives were synthesised by a chemical synthesis method, and two types of AHL derivatives were measured and screened by crystal violet staining assay, which have more obvious inhibitory effects on A. ferrooxidans biofilms under arsenic environment. Their structures were verified through IR and NMR identification. The morphological changes of A. ferrooxidans under the influence of the AHL derivatives were compared. In addition, the effects of AHL derivatives(0.1 μg/mL and 1 μg/mL) on membrane formation of A. ferrooxidans under high concentration of arsenic resistance(1,600 mg/L) were explored. Solid experimental data firstly showed that a portion of logarithmic microorganisms were ruptured under the effect of high arsenic concentration. Secondly, the volume of the cell shrank and the number of extracellular polymeric substances decreased after the addition of the AHL derivatives at high concentrations. Therefore, we found here that two derivatives used at concentrations of 0.1 μg/mL and 1 μg/m L accompanied with high concentration of arsenic can both effectively restrict biofilms formation by A. ferrooxidans. 展开更多
关键词 acetylated homoserine lactone chemical synthesis Acidithiobacillus ferrooxidans Quorum sensing arsenic contamination
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