晚期糖化终产物(AGE)是蛋白质非酶糖化和氧化的终末产物。对糖尿病慢性并发症(DCC)的发生有重要作用。AGE 的形成及和其受体(RAGE)的结合是 AGE 引起糖尿病慢性并发症的主要和关键途径之一,可能是 DCC、衰老等的共同通路。本研究的目的...晚期糖化终产物(AGE)是蛋白质非酶糖化和氧化的终末产物。对糖尿病慢性并发症(DCC)的发生有重要作用。AGE 的形成及和其受体(RAGE)的结合是 AGE 引起糖尿病慢性并发症的主要和关键途径之一,可能是 DCC、衰老等的共同通路。本研究的目的是为了观察并比较一些中西药物对外源性 AGE 在正常小鼠身上引起的氧化应激的抑制作用,从而建立一个快速、简便、准确的方法,用来快速筛选对糖尿病慢性并发症有抑制作用的中西药物。展开更多
This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeu...This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The individuals of E chinensis were exposed to 200 and 1000 cells mL-1 of A. tamarense with their superoxide dismutase （SOD）, glutathione S-transferase （GST） activities, malonyldialdehyde （MDA） concentration, and caspase gene （FcCasp） expression in hepatopancreas determined at 12, 24, 48, 72 and 96 h. In addition, apoptosis in hepatopancreas of E chinensis at 96 h after exposure was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay. The hepatopancreatic SOD and GST activities of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense showed a bell-shaped response to exposure time. The hepatopancreatic MDA concentration ofF. chinensis exposed to 1000 cellsmL-1 ofA. tamarense increased gradually from 48 to 96h, and such a trend corresponded to the decrease of GST activity. The hepatopancreatic FcCasp transcript abundance of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense was positively and linearly correlated to MDA concentration. Results of TUNEL assay showed that exposure to 1000 cells mL-1 of A. tamarense induced apoptosis in the hepatopancreas of E chinensis. Our study revealed that A. tamarense exposure influenced the antioxidative status ofF. chinensis and caused lipid peroxidation and apoptosis in the hepatopancreas of shrimp.展开更多
类 m RNA RNA (m RNA- like RNA)是一类 3′端有 Poly A ,无典型 ORF,不编码蛋白质 ,而直接在 RNA水平上发挥作用的 RNA分子。近年来的研究表明各种不同的类 m RNA RNA广泛存在于不同的细胞内 ,并发挥着重要的调控作用。它们与细胞的生...类 m RNA RNA (m RNA- like RNA)是一类 3′端有 Poly A ,无典型 ORF,不编码蛋白质 ,而直接在 RNA水平上发挥作用的 RNA分子。近年来的研究表明各种不同的类 m RNA RNA广泛存在于不同的细胞内 ,并发挥着重要的调控作用。它们与细胞的生长和分化、胚胎的发育。展开更多
文摘晚期糖化终产物(AGE)是蛋白质非酶糖化和氧化的终末产物。对糖尿病慢性并发症(DCC)的发生有重要作用。AGE 的形成及和其受体(RAGE)的结合是 AGE 引起糖尿病慢性并发症的主要和关键途径之一,可能是 DCC、衰老等的共同通路。本研究的目的是为了观察并比较一些中西药物对外源性 AGE 在正常小鼠身上引起的氧化应激的抑制作用,从而建立一个快速、简便、准确的方法,用来快速筛选对糖尿病慢性并发症有抑制作用的中西药物。
基金supported by Earmarked Fund for Modern Agro-industry Technology Research System of China (Grant No. CARS-47)Special Fund for Agroscientific Research in the Public Interest of China (Grant No. 201103034)the National ‘863’ Project of China (Grant No. 2012AA10A409)
文摘This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The individuals of E chinensis were exposed to 200 and 1000 cells mL-1 of A. tamarense with their superoxide dismutase （SOD）, glutathione S-transferase （GST） activities, malonyldialdehyde （MDA） concentration, and caspase gene （FcCasp） expression in hepatopancreas determined at 12, 24, 48, 72 and 96 h. In addition, apoptosis in hepatopancreas of E chinensis at 96 h after exposure was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay. The hepatopancreatic SOD and GST activities of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense showed a bell-shaped response to exposure time. The hepatopancreatic MDA concentration ofF. chinensis exposed to 1000 cellsmL-1 ofA. tamarense increased gradually from 48 to 96h, and such a trend corresponded to the decrease of GST activity. The hepatopancreatic FcCasp transcript abundance of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense was positively and linearly correlated to MDA concentration. Results of TUNEL assay showed that exposure to 1000 cells mL-1 of A. tamarense induced apoptosis in the hepatopancreas of E chinensis. Our study revealed that A. tamarense exposure influenced the antioxidative status ofF. chinensis and caused lipid peroxidation and apoptosis in the hepatopancreas of shrimp.
文摘类 m RNA RNA (m RNA- like RNA)是一类 3′端有 Poly A ,无典型 ORF,不编码蛋白质 ,而直接在 RNA水平上发挥作用的 RNA分子。近年来的研究表明各种不同的类 m RNA RNA广泛存在于不同的细胞内 ,并发挥着重要的调控作用。它们与细胞的生长和分化、胚胎的发育。
