To estimate the antioxidant activities of Peanut polypeptides （PPs） by using a chemiluminescence （CL） method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide wa...To estimate the antioxidant activities of Peanut polypeptides （PPs） by using a chemiluminescence （CL） method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion （IC50=9.68±0.12 mg/ml）. PPs could scavenge hydroxide radical effectively （the IC50 value was 46.06±0.08 μg/ml）. PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value （0.17±0.07 mg/ml）. PPs （the IC50 value was0.72±0.11 mg/ml） were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.展开更多
文摘To estimate the antioxidant activities of Peanut polypeptides （PPs） by using a chemiluminescence （CL） method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion （IC50=9.68±0.12 mg/ml）. PPs could scavenge hydroxide radical effectively （the IC50 value was 46.06±0.08 μg/ml）. PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value （0.17±0.07 mg/ml）. PPs （the IC50 value was0.72±0.11 mg/ml） were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.
