心肌梗死患者血清脂蛋白氧化易感性及高密度脂蛋白氧化抑制能力分析

目的 了解心肌梗死存活患者 (MIS)血清脂蛋白氧化易感性以及高密度脂蛋白 (HDL)对低密度脂蛋白 (LDL)氧化抑制能力的变化。方法 采用密度梯度超速离心分离 33例MIS患者的血清脂蛋白组分 (VLDL ,LDL ,HDL) ,用A2 34nm光吸收鉴定脂蛋白氧化易感性 ,硫代巴比妥酸值 (TBARS)分析脂蛋白脂质过氧化程度 ,并与正常人 (44例 )进行了比较。结果 MIS患者VLDL、LDL组分脂质过氧化程度明显高于正常人 (P <0 0 1) ,氧化延滞时间明显缩短 (P <0 0 0 1) ,HDL组分对LDL氧化抑制能力显著降低 (P <0 0 1)。结论 MIS患者VLDL ,LDL组分氧化易感性增加 ,HDL抗氧化能力受损 。

乌饭树叶提取物的抗氧化能力探讨

对乌饭树叶提取物的抗氧化能力进行了研究。以VC为对照,分别测定了乌饭树叶精提物和粗提物的还原力、清除DPPH.自由基能力和抑制脂质过氧化能力。结果表明,在还原力和抑制脂质过氧化能力上与Vc相近,约为Vc的90%;而在清除DPPH.自由基的能力上,是Vc的1.4倍。

小麦胚活性肽体外抗氧化活性研究

在还原能力、清除[DPPH.]自由基能力、螯合亚铁能力和抑制亚油酸自氧化能力4种体系下比较了工业生产的小麦胚活性肽与工业生产的其他植物源活性肽的抗氧化活性。研究结果表明:工业生产的小麦胚活性肽的还原能力和清除[DPPH.]自由基能力都高于其他几种植物源活性肽,其中清除[DPPH.]自由基能力的IC50达到1.36 mg/mL;在质量浓度为1 mg/mL时,对亚铁离子的螯合率为40%;抑制亚油酸自氧化能力和其他几种活性肽相差不大。总体来说,工业生产的小麦胚活性肽有很好的体外抗氧化活性。

乌饭树叶提取物抗氧化活性研究及组分分析

采用乙醇浸提、大孔树脂纯化制备乌饭树叶提取物,考察其在不同体系中的抗氧化活性,并对提取物的主要成分进行HPLC分析。结果表明:乌饭树叶提取物具有较高的抗氧化能力,其清除DPPH自由基能力与Vc相当,总抗氧化能力、还原力、抗脂质过氧化能力和清除羟自由基能力与待测物浓度存在明显的量效关系。HPLC结果表明,乌饭树叶提取物中主要存在异槲皮苷、槲皮素和山奈酚等3种黄酮类化合物,其含量分别为267.8,61.6和98.0 mg/g。

Phytochemical composition, biological potential and enzyme inhibition activity of Scandix pecten-veneris L.

Objective： Scandix pecten-veneris L. is a less studied wild edible herb and is considered an extinct plant species in many parts of the world. This study was designed to evaluate its phytochemical composition and biological potential of S. pecten-veneris L. Methods： Phytochemicals including alkaloids, fiavonoids, polyphenols, and tannins were determined in extracts of S. pecten-veneris. Antioxidant activity was determined using 2,2-diphenyl-1- picrylhydrazyl （DPPH）, while reducing power was tested by ferric reducing/antioxidant power （FRAP） assay. Antimi- crobial activity against seven bacterial and four fungal strains was evaluated using agar well diffusion assay. Enzymes inhibition study was performed for urease, phosphodiesterase-I, and catalase-II. Results： S. pecten-veneris showed moderate antiradical activity and reducing potential of hydroxyl radicals to about 20% of the initial value. The antiox- idant activity of various extracts of S. pecten-veneris showed a linear correlation with total phenolic contents in the order of water〉n-butanol〉chloroform〉ethyl acetate〉methanol extracts. S. pecten-veneris leaves showed the highest inhibitory activity against Staphylococcus aureus while the highest antifungal activity was observed against Candida albicans. The plant extract was most potent against urease enzymes but showed moderate activity against phosphodiestrase-I and carbonic anhydrase-II. Conclusions： Our data demonstrate that in addition to its culinary uses, S. pecten-veneris has good medicinal potential and hence could be used for treating some specific health ailments.