Activities of selected soil enzymes (invertase, acid phosphatase, proteinase,catalase, peroxidase and polyphenoloxi-dase) were determined under different spruce forests withrestoration histories of 5, 13, 18, 23, 27 y...Activities of selected soil enzymes (invertase, acid phosphatase, proteinase,catalase, peroxidase and polyphenoloxi-dase) were determined under different spruce forests withrestoration histories of 5, 13, 18, 23, 27 years and an old growth forest over 400 years old in theeastern Qinghai-Tibetan Plateau, China, and their possible use as indicators of ecosystems healthwere analyzed. Plots 10 X 10 m with 4 replications were established to investigate three hypotheses:soil enzyme activities a) would increase with the restoration process; b) would be greater insurface soils than at lower depths; and c) would be correlated to selected physicochemicalproperties. Results showed that as the forests developed after restoration, invertase and peroxidaseactivities usually increased up to the 23 year point. Also soil enzyme activities were associatedwith surface soils and decreased with depths, suggesting that in earlier restoration stages surfaceaddition of organic fertilizer to soils might be more effective than additions at depth. In the 0-20cm soil, there were significant correlations (P < 0.01 or < 0.05) between some soil enzymeactivities and some selected chemical properties. Therefore, temporal changes in enzyme activitiesshould be included as an indicator when evaluating sustainable forest management practices.展开更多
Phase behavior of paclitaxel in solvent mixtures of dichloromethane and supercritical carbon dioxide was investigated using a supercritical phase monitor.Cloud point pressures were determined as a function of temperat...Phase behavior of paclitaxel in solvent mixtures of dichloromethane and supercritical carbon dioxide was investigated using a supercritical phase monitor.Cloud point pressures were determined as a function of temperature,pressure and paclitaxel content from 313.1 to 343.1K and pressures up to 33.52 MPa.The ternary mixtures exhibit a typical lower critical solution temperature behavior.When paclitaxel content increases,the single-phase region shrinks in size.Three cubic equations of state(Redlich-Kworng,Soave-Redlich-Kwong and Peng-Robinson equation of state) coupled with the van der Waals one-fluid mixing rules were selected to correlate the experimental data.The results indicate that SRK EOS coupled with two binary interaction parameters kij and lij can predict paclitaxel solubility for the best fit of experimental data.展开更多
Objective: The aim of the study was to investigate the relationship between susceptibility of paclitaxel-based regimen and gene polymorphisms of cytochrome oxidase CYP3A4 for advanced gastric cancer. Methods: Peripher...Objective: The aim of the study was to investigate the relationship between susceptibility of paclitaxel-based regimen and gene polymorphisms of cytochrome oxidase CYP3A4 for advanced gastric cancer. Methods: Peripheral venous blood sample of 53 advanced gastric cancer patients were enrolled to test the mutation of CYP3A4 gene by denaturing high performance liquid chromatography(DHPLC) and DNA sequencing. The relation between the efficacy of paclitaxel-based regimen and CYP3A4 gene polymorphisms was further analyzed. Results: DHPLC indicated that among the 53 patients, 21 cases showed biomodal type(mutation) and 32 cases were of unimodal type(wild-type). Sequencing results showed that the deletion mutation was found at the 27 th basic group of C in exon 10 of CYP3A4 gene. The response rate(RR) and disease control rate(DCR) of wild-type group were 40.6% and 84.4%, while in mutation group they were 33.3% and 85.7%, respectively, with no significances between the two groups(P > 0.05). Of all 53 cases, the median progression-free survival(PFS) was 6.5 months(95% CI: 3.576–9.424 months), and the median overall survival(OS) was 11.0 months(95% CI: 6.955–15.045 months). The median PFS and OS in wild-type group had no differences compared with those in mutation group(7.0 months vs. 7.0 months, P > 0.05; 10.0 months vs. 14.0 months, P > 0.05). Between wild-type and mutation groups, the median PFS of patients applied with oxaliplatin containing regimen and the median OS in patients applied with/without oxaliplatin had no significant differences(P > 0.05), while the median PFS in patients received non-oxaliplatin regime had statistical differences(P = 0.024). The median PFS and OS in patients receiving 3-drug or 2-drug regimes had no correlation with CYP3A4 gene polymorphisms. The adverse effects in the two groups were mild, mainly in grades 1–2. The common adverse effects were anorexia, nausea/vomiting and leucopenia. Conclusion: Deletion mutation was located in the 27 th basic group of C in exon 10 of CYP3A4 gene. Paclitaxel-based regime has a trend to prolong the OS of advanced gastric cancer with mutation type.展开更多
The aim was to explore the purification ability of potted taxus for indoor sulfur dioxide pollution. Three taxus cultivars were placed in different rooms. The air in the rooms was sampled continuously at the flow rate...The aim was to explore the purification ability of potted taxus for indoor sulfur dioxide pollution. Three taxus cultivars were placed in different rooms. The air in the rooms was sampled continuously at the flow rate of 0.5 ml/min, and the content of sulfur dioxide was determined by formaldehyde absorbing-pararosaniline spectrophotometry. The obtained linear equation for sulfur dioxide was as follows： y=0.030 1 x＋0.023 5, F=0.999 7. The content of sulfur dioxide in the three rooms placed with different taxus cultivars all declined, suggesting that potted taxus has a certain ability to purify sulfur dioxide. Among the taxus cultivars, Taxus yunnanensis showed the highest purification rate, followed by Taxus chinensis vat. mairei and Taxus media cv. Hicksii.展开更多
基金Project supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (Nos. KZCX3-SW-339 and KSCX1-07) the Ministry of Science and Technology of China (No. 2001CCB00600).
文摘Activities of selected soil enzymes (invertase, acid phosphatase, proteinase,catalase, peroxidase and polyphenoloxi-dase) were determined under different spruce forests withrestoration histories of 5, 13, 18, 23, 27 years and an old growth forest over 400 years old in theeastern Qinghai-Tibetan Plateau, China, and their possible use as indicators of ecosystems healthwere analyzed. Plots 10 X 10 m with 4 replications were established to investigate three hypotheses:soil enzyme activities a) would increase with the restoration process; b) would be greater insurface soils than at lower depths; and c) would be correlated to selected physicochemicalproperties. Results showed that as the forests developed after restoration, invertase and peroxidaseactivities usually increased up to the 23 year point. Also soil enzyme activities were associatedwith surface soils and decreased with depths, suggesting that in earlier restoration stages surfaceaddition of organic fertilizer to soils might be more effective than additions at depth. In the 0-20cm soil, there were significant correlations (P < 0.01 or < 0.05) between some soil enzymeactivities and some selected chemical properties. Therefore, temporal changes in enzyme activitiesshould be included as an indicator when evaluating sustainable forest management practices.
基金Supported by the National Natural Science Foundation of China （21076084 20676046） the Fundamental Research Funds for the Central Universities （2011ZZ0006）
文摘Phase behavior of paclitaxel in solvent mixtures of dichloromethane and supercritical carbon dioxide was investigated using a supercritical phase monitor.Cloud point pressures were determined as a function of temperature,pressure and paclitaxel content from 313.1 to 343.1K and pressures up to 33.52 MPa.The ternary mixtures exhibit a typical lower critical solution temperature behavior.When paclitaxel content increases,the single-phase region shrinks in size.Three cubic equations of state(Redlich-Kworng,Soave-Redlich-Kwong and Peng-Robinson equation of state) coupled with the van der Waals one-fluid mixing rules were selected to correlate the experimental data.The results indicate that SRK EOS coupled with two binary interaction parameters kij and lij can predict paclitaxel solubility for the best fit of experimental data.
基金Supported by grants from the National Natural Science Foundation of Fujian Province(No.2009J01120)Innovation in Medical Foundation of Fujian Province(No.2009-CXB-27)
文摘Objective: The aim of the study was to investigate the relationship between susceptibility of paclitaxel-based regimen and gene polymorphisms of cytochrome oxidase CYP3A4 for advanced gastric cancer. Methods: Peripheral venous blood sample of 53 advanced gastric cancer patients were enrolled to test the mutation of CYP3A4 gene by denaturing high performance liquid chromatography(DHPLC) and DNA sequencing. The relation between the efficacy of paclitaxel-based regimen and CYP3A4 gene polymorphisms was further analyzed. Results: DHPLC indicated that among the 53 patients, 21 cases showed biomodal type(mutation) and 32 cases were of unimodal type(wild-type). Sequencing results showed that the deletion mutation was found at the 27 th basic group of C in exon 10 of CYP3A4 gene. The response rate(RR) and disease control rate(DCR) of wild-type group were 40.6% and 84.4%, while in mutation group they were 33.3% and 85.7%, respectively, with no significances between the two groups(P > 0.05). Of all 53 cases, the median progression-free survival(PFS) was 6.5 months(95% CI: 3.576–9.424 months), and the median overall survival(OS) was 11.0 months(95% CI: 6.955–15.045 months). The median PFS and OS in wild-type group had no differences compared with those in mutation group(7.0 months vs. 7.0 months, P > 0.05; 10.0 months vs. 14.0 months, P > 0.05). Between wild-type and mutation groups, the median PFS of patients applied with oxaliplatin containing regimen and the median OS in patients applied with/without oxaliplatin had no significant differences(P > 0.05), while the median PFS in patients received non-oxaliplatin regime had statistical differences(P = 0.024). The median PFS and OS in patients receiving 3-drug or 2-drug regimes had no correlation with CYP3A4 gene polymorphisms. The adverse effects in the two groups were mild, mainly in grades 1–2. The common adverse effects were anorexia, nausea/vomiting and leucopenia. Conclusion: Deletion mutation was located in the 27 th basic group of C in exon 10 of CYP3A4 gene. Paclitaxel-based regime has a trend to prolong the OS of advanced gastric cancer with mutation type.
基金Supported by Key Science and Technology Program for Social Development of Guizhou Province(QKHSY[2013]3148)~~
文摘The aim was to explore the purification ability of potted taxus for indoor sulfur dioxide pollution. Three taxus cultivars were placed in different rooms. The air in the rooms was sampled continuously at the flow rate of 0.5 ml/min, and the content of sulfur dioxide was determined by formaldehyde absorbing-pararosaniline spectrophotometry. The obtained linear equation for sulfur dioxide was as follows： y=0.030 1 x＋0.023 5, F=0.999 7. The content of sulfur dioxide in the three rooms placed with different taxus cultivars all declined, suggesting that potted taxus has a certain ability to purify sulfur dioxide. Among the taxus cultivars, Taxus yunnanensis showed the highest purification rate, followed by Taxus chinensis vat. mairei and Taxus media cv. Hicksii.
