Protein components of which p I <8.3 and molecular weights 35±2 kD and 25±2 kD may be degradation products of GO isozyme 40±2 kD subunit.But protein band of which p I >8 3 and molecular weight 67&...Protein components of which p I <8.3 and molecular weights 35±2 kD and 25±2 kD may be degradation products of GO isozyme 40±2 kD subunit.But protein band of which p I >8 3 and molecular weight 67±2 kD from the proteins containing GOⅢ and GOⅡ was stable.There was a cross immunoreaction between specific GOⅠ antibody and only 40±2 kD protein band from crude protein of spinach green leaves assayed by SDS PAGE Western blot.Specific antigen from spinach crude protein immunoadsorbed by specific GOⅠ antibody showed four bands with the molecular weights 67±2 kD,40±2 kD,38±2 kD,and 25±2 kD assayed by SDS PAGE, Western blot using the antibody against the protein containing GOⅡ.So 67±2 kD protein could be immunoadsorbed by specific GOⅠ antibody.By native PAGE and SDS PAGE,Western blot using the antibody against the protein containing GOⅡ,rice GOⅡ and a rice 67 kD protein of which p I >8 3 was confirmed to be induced by glycolate substrate.On the results above,a new theory was put forwards that spinach GOⅡ consisted of 67±2 kD and 40±2 kD subunits.展开更多
为进一步认识植物对酸雨胁迫的适应性机制,本文选择具有较强耐酸性作物水稻为试材,研究酸雨(AR)(p H 3.5和p H 2.5)对水稻根系抗氧化酶活性及其同工酶组成的影响.发现AR胁迫5 d后,p H 3.5组水稻幼苗根系中SOD(超氧化物歧化酶)、CAT(过...为进一步认识植物对酸雨胁迫的适应性机制,本文选择具有较强耐酸性作物水稻为试材,研究酸雨(AR)(p H 3.5和p H 2.5)对水稻根系抗氧化酶活性及其同工酶组成的影响.发现AR胁迫5 d后,p H 3.5组水稻幼苗根系中SOD(超氧化物歧化酶)、CAT(过氧化氢酶)、POD(过氧化物酶)活性均上升,且同工酶谱条带均增粗变亮,H_2O_2、O_2^(·-)、MDA含量和质膜透性均增加,根长、根表面积、根体积、根冠比均减小;而p H 2.5组根系中虽SOD、CAT活性上升但POD活性下降,其中SOD、CAT同工酶谱条带变粗变亮且较p H 3.5组更粗更亮而POD 3、POD 4、POD 5条带明显变细变暗,O_2^(·-)、H_2O_2、MDA含量和质膜透性与根系生长各指标降幅均大于p H 3.5组.恢复5 d后,p H3.5组下水稻根系中SOD、CAT、POD、H_2O_2、O_2^(·-)、MDA含量和质膜透性恢复至对照水平,而p H 2.5引发的活性氧伤害未恢复.因此,AR胁迫下水稻幼苗根系SOD、CAT、POD同工酶表达量增加促使活性增强有助于清除AR诱发的活性氧积累,增强植物对酸雨的耐受性,调控能力受酸雨强度限制.展开更多
基金国家自然科学基金 (No .3 980 0 0 0 9)广东省自然科学基金 (No .0 114 6 9)资助
文摘Protein components of which p I <8.3 and molecular weights 35±2 kD and 25±2 kD may be degradation products of GO isozyme 40±2 kD subunit.But protein band of which p I >8 3 and molecular weight 67±2 kD from the proteins containing GOⅢ and GOⅡ was stable.There was a cross immunoreaction between specific GOⅠ antibody and only 40±2 kD protein band from crude protein of spinach green leaves assayed by SDS PAGE Western blot.Specific antigen from spinach crude protein immunoadsorbed by specific GOⅠ antibody showed four bands with the molecular weights 67±2 kD,40±2 kD,38±2 kD,and 25±2 kD assayed by SDS PAGE, Western blot using the antibody against the protein containing GOⅡ.So 67±2 kD protein could be immunoadsorbed by specific GOⅠ antibody.By native PAGE and SDS PAGE,Western blot using the antibody against the protein containing GOⅡ,rice GOⅡ and a rice 67 kD protein of which p I >8 3 was confirmed to be induced by glycolate substrate.On the results above,a new theory was put forwards that spinach GOⅡ consisted of 67±2 kD and 40±2 kD subunits.
文摘为进一步认识植物对酸雨胁迫的适应性机制,本文选择具有较强耐酸性作物水稻为试材,研究酸雨(AR)(p H 3.5和p H 2.5)对水稻根系抗氧化酶活性及其同工酶组成的影响.发现AR胁迫5 d后,p H 3.5组水稻幼苗根系中SOD(超氧化物歧化酶)、CAT(过氧化氢酶)、POD(过氧化物酶)活性均上升,且同工酶谱条带均增粗变亮,H_2O_2、O_2^(·-)、MDA含量和质膜透性均增加,根长、根表面积、根体积、根冠比均减小;而p H 2.5组根系中虽SOD、CAT活性上升但POD活性下降,其中SOD、CAT同工酶谱条带变粗变亮且较p H 3.5组更粗更亮而POD 3、POD 4、POD 5条带明显变细变暗,O_2^(·-)、H_2O_2、MDA含量和质膜透性与根系生长各指标降幅均大于p H 3.5组.恢复5 d后,p H3.5组下水稻根系中SOD、CAT、POD、H_2O_2、O_2^(·-)、MDA含量和质膜透性恢复至对照水平,而p H 2.5引发的活性氧伤害未恢复.因此,AR胁迫下水稻幼苗根系SOD、CAT、POD同工酶表达量增加促使活性增强有助于清除AR诱发的活性氧积累,增强植物对酸雨的耐受性,调控能力受酸雨强度限制.
