A field experiment, involving lime N (calcium cyanamide, CaCN2) fertilization as a control measure, was conducted to study environmental problems induced by long-term heavy N application in Japanese tea fields. Long-t...A field experiment, involving lime N (calcium cyanamide, CaCN2) fertilization as a control measure, was conducted to study environmental problems induced by long-term heavy N application in Japanese tea fields. Long-term tea cultivation caused serious soil acidification. Seventy-seven percent of the 70 tea fields investigated had soil pH values below 4.0, and 9% below 3.0, with the lowest value of 2.7. Moreover, excess N application in tea fields put a threat to plant growth, induced serious nitrate contamination to local water, and caused high nitrous oxide loss. Compared with the conventional high N application treatment (1100 kg N ha-1) without lime N, the low N application (400 kg N ha-1) with calcium cyanamide effectively stopped soil acidification as well as achieved the same or slightly higher levels in tea yield and in total N and amino acid contents of tea shoots. The application of calcium cyanamide could be a suitable fertilization for the prevention of environmental problems in tea cultivation.展开更多
Objective: To study the effect of nitric oxide-induced tyrosine phosphorylation of large-conductance calcium-activated potassium (BK Ca) channel α subunit on vascular hyporesponsiveness in rats. Methods: A total of 4...Objective: To study the effect of nitric oxide-induced tyrosine phosphorylation of large-conductance calcium-activated potassium (BK Ca) channel α subunit on vascular hyporesponsiveness in rats. Methods: A total of 46 Wistar rats of either sex, weighing 250 g±20 g, were used in this study. Models of vascular hyporesponsiveness induced by hemorrhagic shock (30 mm Hg for 2 hours) in vivo and by L-arginine in vitro were established respectively. The vascular responsiveness of isolated superior mesenteric arteries to norepinephrine was observed. Tyrosine phosphorylation of BK Ca α subunit was evaluated with methods of immunoprecipitation and Western blotting. Results: In the smooth muscle cells of the superior mesenteric arteries, the expression of BK Ca α subunit tyrosine phosphorylation increased following hemorrhagic shock, and L-arginine could induce BK Ca channel α subunit tyrosine phosphorylation in a time- and dose-dependent manner. L-NAME (Nω-nitro-L-arginine-methyl-ester), a nitric oxide synthetase inhibitor, could partly restore the decreased vasoresponsiveness of the superior mesenteric arteries after hemorrhagic shock in rats. Down-regulating the protein tyrosine phosphorylation with genistein, a widely-used special protein tyrosine kinase inhibitor, could partly improve the decreased vasoresponsiveness of the superior mesenteric arteries induced by L-arginine in vitro, while up-regulating the protein tyrosine phosphorylation with Na3VO4, a protein tyrosine phosphatase inhibitor, could further decrease the nitric oxide-induced vascular hyporesponsiveness, which could be partly ameliorated by 0.1 mmol/L tetrabutylammonium chloride (TEA), a selective BK Ca inhibitor at this concentration. Conclusions: Nitric oxide can induce the tyrosine phosphorylation of BK Ca α subunit, which influences the vascular hyporesponsiveness in hemorrhagic shock rats or induced by L-arginine in vitro.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 40471066)the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX3-SW-417)
文摘A field experiment, involving lime N (calcium cyanamide, CaCN2) fertilization as a control measure, was conducted to study environmental problems induced by long-term heavy N application in Japanese tea fields. Long-term tea cultivation caused serious soil acidification. Seventy-seven percent of the 70 tea fields investigated had soil pH values below 4.0, and 9% below 3.0, with the lowest value of 2.7. Moreover, excess N application in tea fields put a threat to plant growth, induced serious nitrate contamination to local water, and caused high nitrous oxide loss. Compared with the conventional high N application treatment (1100 kg N ha-1) without lime N, the low N application (400 kg N ha-1) with calcium cyanamide effectively stopped soil acidification as well as achieved the same or slightly higher levels in tea yield and in total N and amino acid contents of tea shoots. The application of calcium cyanamide could be a suitable fertilization for the prevention of environmental problems in tea cultivation.
文摘Objective: To study the effect of nitric oxide-induced tyrosine phosphorylation of large-conductance calcium-activated potassium (BK Ca) channel α subunit on vascular hyporesponsiveness in rats. Methods: A total of 46 Wistar rats of either sex, weighing 250 g±20 g, were used in this study. Models of vascular hyporesponsiveness induced by hemorrhagic shock (30 mm Hg for 2 hours) in vivo and by L-arginine in vitro were established respectively. The vascular responsiveness of isolated superior mesenteric arteries to norepinephrine was observed. Tyrosine phosphorylation of BK Ca α subunit was evaluated with methods of immunoprecipitation and Western blotting. Results: In the smooth muscle cells of the superior mesenteric arteries, the expression of BK Ca α subunit tyrosine phosphorylation increased following hemorrhagic shock, and L-arginine could induce BK Ca channel α subunit tyrosine phosphorylation in a time- and dose-dependent manner. L-NAME (Nω-nitro-L-arginine-methyl-ester), a nitric oxide synthetase inhibitor, could partly restore the decreased vasoresponsiveness of the superior mesenteric arteries after hemorrhagic shock in rats. Down-regulating the protein tyrosine phosphorylation with genistein, a widely-used special protein tyrosine kinase inhibitor, could partly improve the decreased vasoresponsiveness of the superior mesenteric arteries induced by L-arginine in vitro, while up-regulating the protein tyrosine phosphorylation with Na3VO4, a protein tyrosine phosphatase inhibitor, could further decrease the nitric oxide-induced vascular hyporesponsiveness, which could be partly ameliorated by 0.1 mmol/L tetrabutylammonium chloride (TEA), a selective BK Ca inhibitor at this concentration. Conclusions: Nitric oxide can induce the tyrosine phosphorylation of BK Ca α subunit, which influences the vascular hyporesponsiveness in hemorrhagic shock rats or induced by L-arginine in vitro.
