In the extremely competitive world of business and manufacturing, solid waste disposal is often overlooked. There are associated costs with excess raw materials, scrap parts, poor use of resources, and outdated materi...In the extremely competitive world of business and manufacturing, solid waste disposal is often overlooked. There are associated costs with excess raw materials, scrap parts, poor use of resources, and outdated materials. All of these contribute to a company＇s solid waste stream, and must be addressed when calculating the costs of disposal. In addition, it is not ecologically or economically sound decision to allow recyclables to enter landfills. Waste analysis and minimization can be very financially advantageous to companies and should be evaluated. This paper discusses a methodology and a case study that uses a solid waste audit process with a focus on urethane recycling.展开更多
The highly potent antitumor agent ansamitocin P3 is a macrolactam isolated from Actinosynnema pretiosum ATCC 31565. A 120-kb DNA fragment was previously identified as the ansamitocin biosynthetic gene cluster, and con...The highly potent antitumor agent ansamitocin P3 is a macrolactam isolated from Actinosynnema pretiosum ATCC 31565. A 120-kb DNA fragment was previously identified as the ansamitocin biosynthetic gene cluster, and contains genes for polyketide assembly, precursor synthesis, post-polyketide synthesis modification, and regulation. Within the biosynthetic gene cluster, asm8 encodes an 1117-amino-acid protein with a high degree of similarity to the large ATP-binding LuxR family-type regulators. In the current study, we determined that inactivation of asm8 by gene replacement in ATCC 31565 resulted in the complete loss of ansamitocin production, and that complementation with a cloned asm8 gene restored ansamitocin biosynthesis. Interestingly, the disruption of asm8 decreased the transcription of genes responsible for 3-amino-5-hydroxybenzoate (AHBA) formation, the starter unit required for ansamitocin biosynthesis. Subsequently, feeding of exogenous AHBA to the asm8 mutant restored ansamitocin biosynthesis, which showed that Asm8 is a specific positive regulator in AHBA biosynthesis. In addition, investigation of asm8 homologs identified two new ansamitocin producers, and inactivation of the asm8 homolog in A. pretiosum ATCC 31280 abolished ansamitocin production in this strain. Characterization of the positive regulator Asm8 and discovery of the two new ansamitocin producers paves the way for further improving production of this important antitumor agent.展开更多
The necessary and sufficient conditions under which a ring satisfies regular power-substitution are investigated. It is shown that a ring R satisfies regular powersubstitution if and only if a-b in R implies that ther...The necessary and sufficient conditions under which a ring satisfies regular power-substitution are investigated. It is shown that a ring R satisfies regular powersubstitution if and only if a-b in R implies that there exist n ∈ N and a U E GLn （R） such that aU = Ub if and only if for any regular x ∈ R there exist m,n ∈ N and U ∈ GLn（R） such that x^mIn = xmUx^m, where a-b means that there exists x,y, z∈ R such that a =ybx, b = xaz and x= xyx = xzx. It is proved that every directly finite simple ring satisfies regular power-substitution. Some applications for stably free R-modules are also obtained.展开更多
文摘In the extremely competitive world of business and manufacturing, solid waste disposal is often overlooked. There are associated costs with excess raw materials, scrap parts, poor use of resources, and outdated materials. All of these contribute to a company＇s solid waste stream, and must be addressed when calculating the costs of disposal. In addition, it is not ecologically or economically sound decision to allow recyclables to enter landfills. Waste analysis and minimization can be very financially advantageous to companies and should be evaluated. This paper discusses a methodology and a case study that uses a solid waste audit process with a focus on urethane recycling.
基金supported by grants from the Ministry of Science and Technology of China (2012CB721005, 2012AA02A706, 2011ZX08009-001, 2012AA022107)the National Natural Science Foundation of China (31070070, 31121064)+1 种基金Ministry of Education of China (20110073110048)the SJTU-UM Collaborative Program
文摘The highly potent antitumor agent ansamitocin P3 is a macrolactam isolated from Actinosynnema pretiosum ATCC 31565. A 120-kb DNA fragment was previously identified as the ansamitocin biosynthetic gene cluster, and contains genes for polyketide assembly, precursor synthesis, post-polyketide synthesis modification, and regulation. Within the biosynthetic gene cluster, asm8 encodes an 1117-amino-acid protein with a high degree of similarity to the large ATP-binding LuxR family-type regulators. In the current study, we determined that inactivation of asm8 by gene replacement in ATCC 31565 resulted in the complete loss of ansamitocin production, and that complementation with a cloned asm8 gene restored ansamitocin biosynthesis. Interestingly, the disruption of asm8 decreased the transcription of genes responsible for 3-amino-5-hydroxybenzoate (AHBA) formation, the starter unit required for ansamitocin biosynthesis. Subsequently, feeding of exogenous AHBA to the asm8 mutant restored ansamitocin biosynthesis, which showed that Asm8 is a specific positive regulator in AHBA biosynthesis. In addition, investigation of asm8 homologs identified two new ansamitocin producers, and inactivation of the asm8 homolog in A. pretiosum ATCC 31280 abolished ansamitocin production in this strain. Characterization of the positive regulator Asm8 and discovery of the two new ansamitocin producers paves the way for further improving production of this important antitumor agent.
基金supported by the grant of Hangzhou Normal University (No.200901).
文摘The necessary and sufficient conditions under which a ring satisfies regular power-substitution are investigated. It is shown that a ring R satisfies regular powersubstitution if and only if a-b in R implies that there exist n ∈ N and a U E GLn （R） such that aU = Ub if and only if for any regular x ∈ R there exist m,n ∈ N and U ∈ GLn（R） such that x^mIn = xmUx^m, where a-b means that there exists x,y, z∈ R such that a =ybx, b = xaz and x= xyx = xzx. It is proved that every directly finite simple ring satisfies regular power-substitution. Some applications for stably free R-modules are also obtained.
