“氨基酸纸层析分离方法”实验教学设计

讨论了"氨基酸纸层析分离方法"实验教学的改革问题,调整实验步骤以节约教学时间;增加实验内容以增强实验的研究性、定量分析的可靠性;设置不同的药品成分,通过实验教学培养学生认真、诚实的科学研究精神。

转氨基作用与氨基酸纸层析实验的改进

目的改进转氨基作用与氨基酸纸层析方式和方法,提高实验效率。方法在新的转氨基反应容器和点样方式下,比较缓冲液类型、转氨基时间、肝匀浆浓度及保存时间、磷酸吡哆醛用量对转氨基的影响。结果适当缩短反应时间、适当稀释和保存肝匀浆对转氨基影响较小;缓冲液类型和磷酸吡哆醛不影响转氨基。结论本研究改进了转氨基反应条件和点样方式,提高了实验效率,改善了实验结果。

改良纸层析法测定发酶液中异亮氨酸的含量

传统的氨基酸纸层析法是以特制的滤纸为支持物,用特殊配方的溶剂混合液作展层剂,然后喷刷显色剂茚三酮,烘烤显色,展层与显色分开操作,给实验带来不便。本文对把显色剂直接加到展层剂中进行了研究,用该方法定量测定了发酵液中的异亮氨酸含量。实验表明:改良后的纸层析法简化了操作,节约试剂,测定L-异亮氨酸含量具有较高的准确度和精密度,适合在L-异亮氨酸生产中应用。

从实验报告撰写入手 培养学生综合能力

指出学生生物化学实验报告中存在的主要问题,以大量的实例总结生物化学实验报告的撰写方法,并从端正态度、做好预习、团队合作、教师批改、网上讨论和课堂讲评等几个方面,对如何保证学生写好实验报告进行分析。

Colorimetric Method for Determination of Amino Acids on Thin Layer and Filter Paper Chromatography Using a Modified Ninhydrin Reagent

A simple and rapid colorimetric method for the and Whatman filter paper chromatography is described. determination of some amino acids on thin layer chromatography （TLC） The studied compounds are cysteine, proline, methionine, tryptophan glutmine, glycine, thyrosine, threonine, leucine and lysine. The method is based on the formation of color from the reaction of stannous chloride （SnCI2） and ninhydrin reagents. The procedure has been successfully applied to the assay of the standard amino acid preparation after TLC separation, and the results of the studied compounds were clear and favorable, comparing to the official methods. However, the improved TLC is much more efficient for the detection of amino acid. The main advantage of this method is that the detection of amino acids can be preformed visually.