Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with ind...Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with indirect detection . The experiments were carried out with homemade CE apparatus under the following operating conditior conditions: a fused-silica capillary col- umn of 50.0cm effect length and of 75m i.d. was used. 7 organic acids were used as BGAE, and a positive potential of separation in CZE with indirect detection. After optimizing for l3 native amino acids were established. Conclusion The choice of BGAe is an important factor influencing the efficiency of separation in CZE with indiect detection .After optimizing the separation conditions a baseline separation for 13 native amino acids is obtained.展开更多
As benzoxazin-4-ones and quinazolines linked to indole nucleus acting as a good pharmacophore, the synthesis of these compounds has significant meaning. The key intermediates 2-（2＇, 5＇-disubstituted-indol-2＇-yl...As benzoxazin-4-ones and quinazolines linked to indole nucleus acting as a good pharmacophore, the synthesis of these compounds has significant meaning. The key intermediates 2-（2＇, 5＇-disubstituted-indol-2＇-yl）-4H-3, 1-benzoxazin-4-ones （3） were synthesized from cyclocondensation of indole-2-carbonyl chlorides （2） and anthranilic acid. Compound （3） on reaction with thiosemicarbazide and o-phenylene diamine afforded the compound （4） and （6） respectively. Compound （4） subjected to intramolecular cyclization under thermal conditions above its melting point afforded the compound （5）. Similarly compound （3） on fusion with o- phenylene diamine gave compound （7）. Structures of these compounds were confirmed by their spectral studies. The compounds were screened for their antimicrobial activity and the results were reported.展开更多
A copper-catalyzed highly stereoselective cyclopropanation of 1,2-disubstituted olefins with α-nitrodiazo acetates has been developed, giving the desired products in up to 97 % yields, up to 〉99/1 dr and up to 98 % ...A copper-catalyzed highly stereoselective cyclopropanation of 1,2-disubstituted olefins with α-nitrodiazo acetates has been developed, giving the desired products in up to 97 % yields, up to 〉99/1 dr and up to 98 % ee, which provides an efficient access to the synthesis of optical active cyclopropane α-amino acids and unnatural α-amino acid derivatives.展开更多
文摘Aim To strdy the separation of native amino acids using capillary zone electro- pboresis (CZE) with indirect ultraviolet detecition. Methods 13 native amino acids were sepa- rated by capillary electrophoresis with indirect detection . The experiments were carried out with homemade CE apparatus under the following operating conditior conditions: a fused-silica capillary col- umn of 50.0cm effect length and of 75m i.d. was used. 7 organic acids were used as BGAE, and a positive potential of separation in CZE with indirect detection. After optimizing for l3 native amino acids were established. Conclusion The choice of BGAe is an important factor influencing the efficiency of separation in CZE with indiect detection .After optimizing the separation conditions a baseline separation for 13 native amino acids is obtained.
文摘As benzoxazin-4-ones and quinazolines linked to indole nucleus acting as a good pharmacophore, the synthesis of these compounds has significant meaning. The key intermediates 2-（2＇, 5＇-disubstituted-indol-2＇-yl）-4H-3, 1-benzoxazin-4-ones （3） were synthesized from cyclocondensation of indole-2-carbonyl chlorides （2） and anthranilic acid. Compound （3） on reaction with thiosemicarbazide and o-phenylene diamine afforded the compound （4） and （6） respectively. Compound （4） subjected to intramolecular cyclization under thermal conditions above its melting point afforded the compound （5）. Similarly compound （3） on fusion with o- phenylene diamine gave compound （7）. Structures of these compounds were confirmed by their spectral studies. The compounds were screened for their antimicrobial activity and the results were reported.
基金supported by the National Natural Science Foundation of China(21421091,21432011 and21272250)the Chinese Academy of Sciences
文摘A copper-catalyzed highly stereoselective cyclopropanation of 1,2-disubstituted olefins with α-nitrodiazo acetates has been developed, giving the desired products in up to 97 % yields, up to 〉99/1 dr and up to 98 % ee, which provides an efficient access to the synthesis of optical active cyclopropane α-amino acids and unnatural α-amino acid derivatives.
