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Transfer of Lysine-rich Protein Gene into Rice and Production of Fertile Transgenic Plants 被引量:54
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作者 高越峰 荆玉祥 +3 位作者 沈世华 田世平 匡廷云 Samuel S.M.SUN 《Acta Botanica Sinica》 CSCD 2001年第5期506-511,共6页
Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest effici... Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter. The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. Genomic PCR and Southern blotting analyses showed that the lys gene has been integrated into rice genome. Simultaneously, the results of GUS histochemical assay demonstrated that gus report gene is also expressed in leaves, stems and roots of the transgenic rice plants. Data analysis showed that lysine content in most of the 11 transgenic plants is differently improved, and in one of them increased by 16.04%. 展开更多
关键词 lysine-rich protein gene microprojectile bombardment transgenic rice lysine content
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烧成系统小设备损坏案例分析及其引发的思考
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作者 张中国 《新世纪水泥导报》 CAS 2023年第5期66-68,共3页
热电偶损坏后,系统温度异常变化;氨水喷枪的损坏,导致氨水消耗量的增加。在分析和处理这类事故的过程中引发思考,结论是:做好设备的定期点检与维护工作就显得尤为重要,不能要求设备带病上岗,更也不能忽视设备带病服役,要建立完善关键设... 热电偶损坏后,系统温度异常变化;氨水喷枪的损坏,导致氨水消耗量的增加。在分析和处理这类事故的过程中引发思考,结论是:做好设备的定期点检与维护工作就显得尤为重要,不能要求设备带病上岗,更也不能忽视设备带病服役,要建立完善关键设备定期检查制度和做好维护工作,当好设备检查医生,为生产设备安全、高效、低能耗运行奠定良好基础。 展开更多
关键词 分解炉温度 热电偶 氨水消耗 氨水枪 技术管理
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