Objective] The aim was to explore the potential of Nitzschia capitel ata as excel ent baits and bioenergy, and the optimal light intensity and nitrogen-to-phos-phorus (N/P) ratio suitable for its growth were researc...Objective] The aim was to explore the potential of Nitzschia capitel ata as excel ent baits and bioenergy, and the optimal light intensity and nitrogen-to-phos-phorus (N/P) ratio suitable for its growth were researched as wel . [Method] With light intensity gradient set, Nitzschia capitel ata was cultured with the same nutrients at (25 ±1) ℃ and light cycle at 12 h/d to select optimal light intensity. Then, Nitzschia capitel ata was cultured with the same condition, and nitrogen-to-phospho-rus ratios at 5∶1, 6∶1, 7∶1 and 8∶1. [Result] With light intensity at 3 000 lx, specific growth rate and standing stock achieved the highest at 0.51 d-1 and 7.97×104 cel s/ml. The growth condition with nitrogen-to-phosphorus ratio at 6∶1 was the most suitable for Nitzschia capitel ata growth. [Conclusion] The optimal light intensity and nitrogen-to-phosphorus ratio were 3 000 lx and 6∶1, respectively, for Nitzschia capitel ata.展开更多
This paper studied the effects of different ratios of nitrogen and phospho- rus on the growth and competition of Anabaena sp. strain PCC and chloralla vul- gads (low nitrogen-phosphorus ratio group: N/P=16:1; Mediu...This paper studied the effects of different ratios of nitrogen and phospho- rus on the growth and competition of Anabaena sp. strain PCC and chloralla vul- gads (low nitrogen-phosphorus ratio group: N/P=16:1; Medium low nitrogen-phospho- rus ratio group: N/P=32:1; Medium high nitrogen-phosphorus ratio group: N/P=64:1; High nitrogen-phosphorus ratio group: N/P=320:1). Results suggested that the largest amount of anabaena sp.strain PCC survived in medium high nitrogen-phosphorus ratio group. The nitrogen-phosphorus ratio showed no significant influences on the growth of Chlorella vulgaris, but it exerted dramatic influences on the growth of Chlore/la vulgaris of the mixed cultivation system. The largest amount of Ch/orel/a vulgaris can be found in the medium-high nitrogen-phosphorus ratio group. The inhi- bition parameter of nitrogen-phosphorus on the algae was distinctive. Anabaena sp. strain PCC had advantages in the competition with the low nitrogen-phosphorus ra- tio and medium-low nitrogen-phosphorus ratio. Potential instability existed between anabaena sp.strain PCC and Chlorella vulgaris when the nitrogen to phosphorus ratio was medium-high and high.展开更多
[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on th...[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application.展开更多
Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios o...Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen (nitrate-nitrogen) or organic nitrogen (urea-nitrogen) as the sole nitrogen source at initial N:P ratios ranging from 1:1 to 32:1. The favorable N:P of 16:1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N:P of 4:1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N:P of 4:1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N:P of 4: lwere markedly higher than those from cultures with other N:P ratios (p〈 0.05). The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.展开更多
Inspired by the importance of Redfield-type C:N:P ratios in global soils,we looked for analogous patterns in peatlands and aimed at deciphering the potential affecting factors.By analyzing a suite of peatlands soil da...Inspired by the importance of Redfield-type C:N:P ratios in global soils,we looked for analogous patterns in peatlands and aimed at deciphering the potential affecting factors.By analyzing a suite of peatlands soil data(n = 1031),mean soil organic carbon(SOC),total nitrogen(TN) and total phosphorous(TP) contents were 50.51%,1.45% and 0.13%,respectively,while average C:N,C:P and N:P ratios were 26.72,1186.00 and 46.58,respectively.C:N ratios showed smaller variations across different vegetation coverage and had less spatial heterogeneity than C:P and N:P ratios.No consistent C:N:P ratio,though with a general value of 1245:47:1,was found for entire peatland soils in China.The Northeast China,Tibet,Zoigê Plateau and parts of Xinjiang had high soil SOC,TN,TP,and C:P ratio.Qinghai,parts of the lower reaches of the Yangtze River,and the coast zones have low TP and N:P ratio.Significant differences for SOC,TN,TP,C:N,C:P and N:P ratios were observed across groups categorized by predominant vegetation.Moisture,temperature and precipitation all closely related to SOC,TN,TP and their pairwise ratios.The hydrothermal coefficient(RH),defined as annual average precipitation divided by temperature,positively and significantly related to C:N,C:P and N:P ratios,implying that ongoing climate change may prejudice peatlands as carbon sinks during the past 50 years in China.展开更多
Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was a...Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was always much greater than the theoretical Redfield ratio of 16:1 found below the thermocline zone. It was in general higher near the coast and lower in the central part. With increasing depth, the ratio became smaller and smaller. This distribution pattern is attributed to: 1) the anthropogenic influence of the surface N and P which makes the N/P ratio differ from the normal value; 2) the easy adsorption of P on particles hinders P transport to the central part; 3) below the thermocline zone, the N and P mainly come from the remineralization of the sedimented phytoplankton residues which have almost the theoretical Redfield value and; 4) the existence of the Yellow Sea Bottom Cold Water which inhibits the vertical exchange of the water.展开更多
Field experiments were conducted during 2008-2010 to investigate the ef- fects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape (Brassica napus L.) - Xiangzayou...Field experiments were conducted during 2008-2010 to investigate the ef- fects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape (Brassica napus L.) - Xiangzayou763, and to determine the optimum NPK ratio and dose in Hunan, China. The results showed that NPK ratio (1:0.50:0.50) with 180 kg/hm2 N, 90 kg/hm2 P2O5 and 90 kg/hm2 K20 was the optimum combination for the highest seed yield (2 231.13 kg/hm2) and economic benefit (9 816.97 Yuan/hm2), of which 1 641.23 Yuan/hm2 being set for fertilizer in- vestment (VCR=4.11). Besides, the effects of N, P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical. N had the greatest ef- fect on plant growth of winter oilseed rape, followed by P and K. Total leaf number, green leaf number, leaf length and width, rhizome diameter, branch number, dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments. By correlation and path analysis of yield components, the contribution rate to seed yield was found: effective silique number per plant〉seed number per silique〉l 000-seed weight.展开更多
Fertilizers are heavily applied in orchards of the hilly and mountainous topography of South China and may increase nutrient loadings to receiving waters.A simple runoff collecting system was used to measure the effec...Fertilizers are heavily applied in orchards of the hilly and mountainous topography of South China and may increase nutrient loadings to receiving waters.A simple runoff collecting system was used to measure the effects of different fertilization treatments on total N and P concentrations of surface runoff in a Chinese chestnut (Castanea mollissima Blume) orchard in Dongyuan County,Guangdong Province,China.In such orchards,fertilizer was typically applied in two short furrows or pits on either side of each tree.Treatments included three application depths (surface,10cm and 20 cm),and three application rates (low,median and high).Results showed that 90.5% of the runoff water samples had a total N concentration higher than 0.35 mgL^(-1) and 54.2% had a total P concentration higher than 0.1 mgL^(-1).Fertilizer application at all depths and at all but the lowest rate significantly increased total N and P concentrations in runoff water.Fertilization with chemical compound fertilizer at a soil depth of 20cm produced significantly lower (P<0.05) total N concentration in runoff than both surface and 10-cm depth fertilization,and significantly lower (P<0.05) total P concentration in runoff than surface fertilization.Total N and P concentrations in runoff significantly increased with the application rate of organic fertilizers.With the exception of total P concentrations,which were not significantly different between the control and fertilization at a rate of 119 kg P ha-1 in organic form,all the other fertilization treatments produced significantly higher total N and total P concentrations in runoff than the control.A fertilization depth≥20cm and an application rate≤72 kg N ha^(-1) or 119 kg P ha^(-1) for compound organic fertilizer was suggested to substantially reduce N and P runoff losses from hillslope orchards and to protect receiving waters in South China.展开更多
Much attention has been paid to the stoichiometry of carbon(C), nitrogen(N), and phosphorus(P) because of their significance for plant growth and climate change. However, other nutrients, such as sulfur(S), are often ...Much attention has been paid to the stoichiometry of carbon(C), nitrogen(N), and phosphorus(P) because of their significance for plant growth and climate change. However, other nutrients, such as sulfur(S), are often ignored. In this study, we analyzed the stoichiometry of N, P, and S in leaves of 348 plant species in China's forests. The results show higher N content and higher molar ratios of N/P and P/S in Angiospermae than in Gymnospermae. At the family level, Ulmaceae absorbed more N and P from soils than other families, and Cupressaceae absorbed more S than other families. In addition,except for bamboo and other tropical forests, leaf N and P content of China's forests generally increased from low to middle latitudes and then slightly decreased or plateaued at high latitudes. Plant ecotypes, taxonomic groups, environmental conditions, atmospheric S precipitation, and soil-available N and P significantly affected the distribution and stoichiometry of leaf N, P, and S in China's forests.Our study indicates that China's forests are likely limited by P and S deficiencies which may increase in the future.展开更多
基金Supported by Guizhou Provincial Science&Technology Fund((2010)2067)~~
文摘Objective] The aim was to explore the potential of Nitzschia capitel ata as excel ent baits and bioenergy, and the optimal light intensity and nitrogen-to-phos-phorus (N/P) ratio suitable for its growth were researched as wel . [Method] With light intensity gradient set, Nitzschia capitel ata was cultured with the same nutrients at (25 ±1) ℃ and light cycle at 12 h/d to select optimal light intensity. Then, Nitzschia capitel ata was cultured with the same condition, and nitrogen-to-phospho-rus ratios at 5∶1, 6∶1, 7∶1 and 8∶1. [Result] With light intensity at 3 000 lx, specific growth rate and standing stock achieved the highest at 0.51 d-1 and 7.97×104 cel s/ml. The growth condition with nitrogen-to-phosphorus ratio at 6∶1 was the most suitable for Nitzschia capitel ata growth. [Conclusion] The optimal light intensity and nitrogen-to-phosphorus ratio were 3 000 lx and 6∶1, respectively, for Nitzschia capitel ata.
基金Supported by Modern Agricultural Production Technological System Construction(No:CARS-49)Central Public-Interest Scientific Institution Basal Research Fund(No:2013JBFM06)Jiangsu Wuxi Agricultural Scientific Cooperation Program~~
文摘This paper studied the effects of different ratios of nitrogen and phospho- rus on the growth and competition of Anabaena sp. strain PCC and chloralla vul- gads (low nitrogen-phosphorus ratio group: N/P=16:1; Medium low nitrogen-phospho- rus ratio group: N/P=32:1; Medium high nitrogen-phosphorus ratio group: N/P=64:1; High nitrogen-phosphorus ratio group: N/P=320:1). Results suggested that the largest amount of anabaena sp.strain PCC survived in medium high nitrogen-phosphorus ratio group. The nitrogen-phosphorus ratio showed no significant influences on the growth of Chlorella vulgaris, but it exerted dramatic influences on the growth of Chlore/la vulgaris of the mixed cultivation system. The largest amount of Ch/orel/a vulgaris can be found in the medium-high nitrogen-phosphorus ratio group. The inhi- bition parameter of nitrogen-phosphorus on the algae was distinctive. Anabaena sp. strain PCC had advantages in the competition with the low nitrogen-phosphorus ra- tio and medium-low nitrogen-phosphorus ratio. Potential instability existed between anabaena sp.strain PCC and Chlorella vulgaris when the nitrogen to phosphorus ratio was medium-high and high.
基金Supported by National Science and Technology Support Program(2006BAD05B06-04)~~
文摘[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application.
基金partially supported by Tianjin Marine Revitalization Plan of Science and Technology (No.KJXH2013-16) from Tianjin Oceanic Administration,China
文摘Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sus- tainable production. In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth, chlorophyll content and accumulation of lipids in Dunaliella tertiolecta, experiments were carried out in modified microalgal medium with inorganic nitrogen (nitrate-nitrogen) or organic nitrogen (urea-nitrogen) as the sole nitrogen source at initial N:P ratios ranging from 1:1 to 32:1. The favorable N:P of 16:1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate. Decrease in chlorophyll content were observed at the N:P of 4:1 in both nitrate-N and urea-N cultures. It was also observed that the maximum lipids concentration was obtained at the N:P of 4:1 in both nitrate and urea nutrient medium. The lipid productivity and lipid content of cultures in the urea-N medium at the N:P of 4: lwere markedly higher than those from cultures with other N:P ratios (p〈 0.05). The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D. tertiolecta. Conversely, higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitro- gen to phosphorus. The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumu- lation in D. tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level.
基金Under the auspices of National Key Research Program of China(No.2016YFC0500404-5)National Natural Science Foundation of China(No.41671081,41471081,41671087)Foundation of Jilin Province(No.20140520141JH)
文摘Inspired by the importance of Redfield-type C:N:P ratios in global soils,we looked for analogous patterns in peatlands and aimed at deciphering the potential affecting factors.By analyzing a suite of peatlands soil data(n = 1031),mean soil organic carbon(SOC),total nitrogen(TN) and total phosphorous(TP) contents were 50.51%,1.45% and 0.13%,respectively,while average C:N,C:P and N:P ratios were 26.72,1186.00 and 46.58,respectively.C:N ratios showed smaller variations across different vegetation coverage and had less spatial heterogeneity than C:P and N:P ratios.No consistent C:N:P ratio,though with a general value of 1245:47:1,was found for entire peatland soils in China.The Northeast China,Tibet,Zoigê Plateau and parts of Xinjiang had high soil SOC,TN,TP,and C:P ratio.Qinghai,parts of the lower reaches of the Yangtze River,and the coast zones have low TP and N:P ratio.Significant differences for SOC,TN,TP,C:N,C:P and N:P ratios were observed across groups categorized by predominant vegetation.Moisture,temperature and precipitation all closely related to SOC,TN,TP and their pairwise ratios.The hydrothermal coefficient(RH),defined as annual average precipitation divided by temperature,positively and significantly related to C:N,C:P and N:P ratios,implying that ongoing climate change may prejudice peatlands as carbon sinks during the past 50 years in China.
文摘Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was always much greater than the theoretical Redfield ratio of 16:1 found below the thermocline zone. It was in general higher near the coast and lower in the central part. With increasing depth, the ratio became smaller and smaller. This distribution pattern is attributed to: 1) the anthropogenic influence of the surface N and P which makes the N/P ratio differ from the normal value; 2) the easy adsorption of P on particles hinders P transport to the central part; 3) below the thermocline zone, the N and P mainly come from the remineralization of the sedimented phytoplankton residues which have almost the theoretical Redfield value and; 4) the existence of the Yellow Sea Bottom Cold Water which inhibits the vertical exchange of the water.
基金Supported by the Youth Fund of Orient Science and Technology College,Hunan Agricultural University(14QNZ09)Cultivation Physiology Station of National Technical System in Rape Industrial,National Key Technology Support Program(2012BAD15B04,2010BAD01B01)+1 种基金Special Fund of Government in Hunan Province,National Natural Science Foundation of China(31071851,31101596,3132130)Open Fund Project of Innovation Platform in Colleges and Universities of Hunan Province(12K064)
文摘Field experiments were conducted during 2008-2010 to investigate the ef- fects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape (Brassica napus L.) - Xiangzayou763, and to determine the optimum NPK ratio and dose in Hunan, China. The results showed that NPK ratio (1:0.50:0.50) with 180 kg/hm2 N, 90 kg/hm2 P2O5 and 90 kg/hm2 K20 was the optimum combination for the highest seed yield (2 231.13 kg/hm2) and economic benefit (9 816.97 Yuan/hm2), of which 1 641.23 Yuan/hm2 being set for fertilizer in- vestment (VCR=4.11). Besides, the effects of N, P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical. N had the greatest ef- fect on plant growth of winter oilseed rape, followed by P and K. Total leaf number, green leaf number, leaf length and width, rhizome diameter, branch number, dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments. By correlation and path analysis of yield components, the contribution rate to seed yield was found: effective silique number per plant〉seed number per silique〉l 000-seed weight.
基金Project supported by the Science and Technology Department of Guangdong Province,China (No.2004B33301007)the Rockefeller Brothers Fund,America.
文摘Fertilizers are heavily applied in orchards of the hilly and mountainous topography of South China and may increase nutrient loadings to receiving waters.A simple runoff collecting system was used to measure the effects of different fertilization treatments on total N and P concentrations of surface runoff in a Chinese chestnut (Castanea mollissima Blume) orchard in Dongyuan County,Guangdong Province,China.In such orchards,fertilizer was typically applied in two short furrows or pits on either side of each tree.Treatments included three application depths (surface,10cm and 20 cm),and three application rates (low,median and high).Results showed that 90.5% of the runoff water samples had a total N concentration higher than 0.35 mgL^(-1) and 54.2% had a total P concentration higher than 0.1 mgL^(-1).Fertilizer application at all depths and at all but the lowest rate significantly increased total N and P concentrations in runoff water.Fertilization with chemical compound fertilizer at a soil depth of 20cm produced significantly lower (P<0.05) total N concentration in runoff than both surface and 10-cm depth fertilization,and significantly lower (P<0.05) total P concentration in runoff than surface fertilization.Total N and P concentrations in runoff significantly increased with the application rate of organic fertilizers.With the exception of total P concentrations,which were not significantly different between the control and fertilization at a rate of 119 kg P ha-1 in organic form,all the other fertilization treatments produced significantly higher total N and total P concentrations in runoff than the control.A fertilization depth≥20cm and an application rate≤72 kg N ha^(-1) or 119 kg P ha^(-1) for compound organic fertilizer was suggested to substantially reduce N and P runoff losses from hillslope orchards and to protect receiving waters in South China.
基金support from the National Natural Science Foundation of China(41522207,41571130042)the State’s Key Project of Research and Development Plan of China(2016YFA0601002)
文摘Much attention has been paid to the stoichiometry of carbon(C), nitrogen(N), and phosphorus(P) because of their significance for plant growth and climate change. However, other nutrients, such as sulfur(S), are often ignored. In this study, we analyzed the stoichiometry of N, P, and S in leaves of 348 plant species in China's forests. The results show higher N content and higher molar ratios of N/P and P/S in Angiospermae than in Gymnospermae. At the family level, Ulmaceae absorbed more N and P from soils than other families, and Cupressaceae absorbed more S than other families. In addition,except for bamboo and other tropical forests, leaf N and P content of China's forests generally increased from low to middle latitudes and then slightly decreased or plateaued at high latitudes. Plant ecotypes, taxonomic groups, environmental conditions, atmospheric S precipitation, and soil-available N and P significantly affected the distribution and stoichiometry of leaf N, P, and S in China's forests.Our study indicates that China's forests are likely limited by P and S deficiencies which may increase in the future.