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谷氨酰胺合成酶基因GS1和GS2的高效表达增强转基因水稻对氮素缺乏的耐性(英文) 被引量:28
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作者 孙辉 黄其满 苏金 《植物生理与分子生物学学报》 CAS CSCD 北大核心 2005年第5期492-498,共7页
构建了同时含有胞质谷氨酰胺合成酶(GS1)cDNA和叶绿体谷氨酰胺合成酶(GS2)cDNA的植物表达载体p2GS,通过农杆菌介导法用它们转化了水稻品种“中花10号”的成熟胚愈伤组织,经潮霉素(Hyg)筛选培养及分化再生,获得了抗Hyg的转基因水稻植株。... 构建了同时含有胞质谷氨酰胺合成酶(GS1)cDNA和叶绿体谷氨酰胺合成酶(GS2)cDNA的植物表达载体p2GS,通过农杆菌介导法用它们转化了水稻品种“中花10号”的成熟胚愈伤组织,经潮霉素(Hyg)筛选培养及分化再生,获得了抗Hyg的转基因水稻植株。PCR和基因组Southern杂交分析结果证明,GS1和GS2基因均已经整合到转基因水稻的基因组内。Northern杂交实验结果证实,GS1和GS2基因在转基因水稻的转录水平上得到了有效表达。在以0.7mmol/L的(NH4)2SO4取代了其中氮成分的MS培养基上测试植株生长量,结果表明转基因植株鲜重增长量显著高于对照,证明高效表达GS增强了转基因水稻对土壤氮素缺乏的耐性。 展开更多
关键词 谷氨酰胺合成酶 转基因水稻 氮素缺乏耐性
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浅谈玉米的氮素营养诊断 被引量:1
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作者 巨艳春 《科技促进发展》 2010年第8期275-275,共1页
玉米的高大植株和广大的叶面积显示它能消耗大量的肥料,因此它比小株禾谷类要容易出现缺乏症。玉米生长周期中需要大量的氮肥,易感氮不足。因此也被称为氮指示植物。缺氮是严重影响玉米产量和品质的重要因素之一,尽早对玉米植株缺氮做... 玉米的高大植株和广大的叶面积显示它能消耗大量的肥料,因此它比小株禾谷类要容易出现缺乏症。玉米生长周期中需要大量的氮肥,易感氮不足。因此也被称为氮指示植物。缺氮是严重影响玉米产量和品质的重要因素之一,尽早对玉米植株缺氮做出诊断、及时采取补救措施,对指导玉米生产具有重要的意义。 展开更多
关键词 玉米 氮素营养诊断 氮素缺乏
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卷心菜生理性病害的防治
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作者 唐高霞 《吉林农业》 2000年第5期16-17,共2页
关键词 卷心菜 生理性病害 防治 氮素缺乏 钙素缺乏
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Fertilizer-Induced Advances in Corn Growth Stage and Quantitative Definitions of Nitrogen Deficiencies 被引量:5
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作者 J. ZHANG A. M. BLACKMER +2 位作者 P. M. KYVERYGA B. W. VAN DE WOESTYNE T. M. BLACKMER 《Pedosphere》 SCIE CAS CSCD 2008年第1期60-68,共9页
Evidence that nitrogen (N) fertilization tends to accelerate maturation as well as increase rates of growth has received little attention when diagnosing N deficiencies in corn (Zea mays L.).Such a tendency could be a... Evidence that nitrogen (N) fertilization tends to accelerate maturation as well as increase rates of growth has received little attention when diagnosing N deficiencies in corn (Zea mays L.).Such a tendency could be a potential source of errors when the diagnosis is solely based on comparing plants with different rates of growth.Whether N fertilization could accelerate rates of growth and maturation was tested in a field study with 12 paired plots representing relatively large variability in soil properties and landscape positions.The plots were located under conditions where preplant N fertilization reduced or avoided temporary N shortages for some plants but did not reduce for other plants early in the season.We measured corn heights to the youngest leaf collar,stages of growth and chlorophyll meter readings (CMRs). The added N advanced growth stages as well as increased corn heights and CMRs at any given time.Fertilization effects on corn heights,growth stages and ear weights were statistically significant (P<0.05) despite substantial variability associated with landscape.Reductions in growth due to a temporary shortage of N within a growth stage might be partially offset by longer periods of growth within that stage to physiological maturity.Temporary shortages of N,therefore,may produce symptoms of N deficiency in situations where subsequent additions of N should not be expected to increase yields.Recognition of these two somewhat different effects (i.e.,increase growth rates and advance growth stages) on corn growth could help to define N deficiency more precisely and to improve the accuracy of diagnosing N status in production agriculture. 展开更多
关键词 CHLOROPHYLL CORN FERTILIZER growth stage nitrogen deficiency
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Differences in Physiological Age Affect Diagnosis of Nitrogen Deficiencies in Cornfields
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作者 J. ZHANG A. M. BLACKMER T. M. BLACKMER 《Pedosphere》 SCIE CAS CSCD 2008年第5期545-553,共9页
Many studies have shown that chlorophyll meter readings (CMRs) can be used to diagnose deficiencies of nitrogen (N) during the growth of corn (Zea mays L.) in small-plot trials, but there is need to address addi... Many studies have shown that chlorophyll meter readings (CMRs) can be used to diagnose deficiencies of nitrogen (N) during the growth of corn (Zea mays L.) in small-plot trials, but there is need to address additional problems encountered when diagnoses are made in fields of the size managed in production agriculture. A noteworthy difference between smallplot trials and production agriculture is the extent to which the effects of N are confounded with the effects of other factors such as tillage, landscape, soil organic matter and moisture content. We illustrate how some of these factors can cause differences in the physiological age of plants and introduce errors in the diagnoses of N deficiencies. We suggest methods (measuring the height to the youngest leaf collar and assigning leaf numbers by using the first leaf with pubescence and the ear leaf as references to identify growth stages) for minimizing these errors. The simplified method of growth stage identification can be used to select appropriate plants and leaves for making diagnoses in fields and to distinguish the effects of N from the effects of other factors that influence plant growth. 展开更多
关键词 CHLOROPHYLL corn (Zea mays L.) nitrogen deficiency physiological age
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