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基于同位素示踪和分子生物学技术对潮白河沉积物中氮还原功能特征的研究 被引量:6
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作者 彭强 关傲梅 +1 位作者 齐维晓 曲久辉 《环境科学学报》 CAS CSCD 北大核心 2021年第11期4615-4624,共10页
沉积物氮还原功能对于调控和管理河流氮负荷具有重要作用.本研究选取潮白河不同区域河段表层沉积物作为研究对象,采用^(15)N同位素示踪技术、16S rRNA扩增子测序和实时荧光定量PCR(qPCR)技术,研究了潮白河沉积物中氮还原过程(反硝化、... 沉积物氮还原功能对于调控和管理河流氮负荷具有重要作用.本研究选取潮白河不同区域河段表层沉积物作为研究对象,采用^(15)N同位素示踪技术、16S rRNA扩增子测序和实时荧光定量PCR(qPCR)技术,研究了潮白河沉积物中氮还原过程(反硝化、厌氧氨氧化(anaerobic ammonia oxidation,anammox)和DNRA过程(dissimilatory nitrate reduction to ammonium,异化硝酸盐还原为铵))速率特征、相关功能基因丰度和微生物群落结构特征.结果表明:潮白河沉积物潜在反硝化、anammox、DNRA和N_(2)O释放速率分别为(25.82±0.98)~(142.32±17.06)、(0.58±0.10)~(4.51±0.78)、(2.91±0.46)~(19.59±4.15)和(-1.09±0.17)~(3.30±0.57)nmol·g^(-1)·h^(-1)(以N计,下同).沉积物氮还原以反硝化过程为主(贡献率71.86%~91.65%),DNRA也有较高的贡献率(7.03%~25.32%),anammox过程贡献较小.相关分析表明,氮还原相关功能基因丰度与潮白河沉积物氮还原过程速率存在显著正相关关系,微生物群落中α-变形菌纲、硝基螺旋菌纲、疣微菌纲、脱硫杆菌门、硬壁菌门丰度与沉积物氮还原过程速率存在显著的相关关系. 展开更多
关键词 氮还原过程 潜在速率 功能基因 微生物群落组成 ^(15)N同位素示踪
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Conversion of Fuel-N to N2O and NOx during Coal Combustion in Combustors of Different Scale 被引量:3
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作者 周昊 黄燕 +2 位作者 莫桂源 廖子昱 岑可法 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第9期999-1006,共8页
With focus on investigating the effect of combustor scale on the conversion of fuel-N to NOx and N20, experiments are carried out in three combustors, including single coal particle combustion test rig, laboratory sca... With focus on investigating the effect of combustor scale on the conversion of fuel-N to NOx and N20, experiments are carried out in three combustors, including single coal particle combustion test rig, laboratory scale circulating fluidized-bed boiler (CFB) and full scale CFB in this work. For single coal particle combustion, the majority of f-uel-N (65%-82%) is released as NOx, while only a little (less than 8%) fuel-N yields N20. But in labora- tory scale CFB, the conversion of fuel-N to N20 is increases, but the conversion of fuel-N to NOx is quite less than that of single coal particle combustion. This is because much char in CFB can promote the NOx reduction by in- creasing N20 formation. In full scale CFB, both of the conversion of fuel-N to NOx and the conversion of fuel-N to N20 are smaller than laboratory scale CFB. 展开更多
关键词 fuel-N N2O NOx COAL fluidized bed
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