[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete...[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete primary structure and high purity was obtained from the sheep blood clot after the steps of cutting the sheep blood clot with ophthalmic scissors, cell lysis with tissue DNA extracts and digested by proteinase K, extracting with phenol/chloroform and precipitating with ethanol were performed. [ Result] The concentration of the extracted DNA was 159.90 ±0.70 ng/μl and the ratio of the A260/A280 was 1.80 ＋0.01. The sheep microsatellite locus of BM203 was amplified by using the extracted DNA from the sheep blood clot as template of PCR, and the PCR result was perfect. [Conclusion]This method is simple and feasible, the quantity and quality of the extracted DNA can satisfy the demands for the subsequent researches. It is worth to extending and using for reference.展开更多
作为Sigma-Aldrich Co Ltd一部分的Fluka Chemicals研制出一种新的RNA分离盒,其Micro Selelct区具有快速、一步提取RNA的功能。这种盒使用guanidiam硫氰酸盐-酚-氯仿提取法,能获得纯RNA,不含DNA且污染蛋白含量低于用guanidium-CsC1法制...作为Sigma-Aldrich Co Ltd一部分的Fluka Chemicals研制出一种新的RNA分离盒,其Micro Selelct区具有快速、一步提取RNA的功能。这种盒使用guanidiam硫氰酸盐-酚-氯仿提取法,能获得纯RNA,不含DNA且污染蛋白含量低于用guanidium-CsC1法制备的样品。因为没有离心步骤,所以能同时加工大量样品。Fluka介绍说,这种盒可应用于小规模和大规模制备RNA。展开更多
基金Supported by Natural Science Foundation of Shanxi Province(2007011081 )Returning Brains Project in Shanxi Province(2007066 )Agricultural Science and Technology Achievement Transformation Fund Project(2008GB2A300032)~~
文摘[ Objective] The aim was to establish the method of extracting genomic DNA from sheep blood clot on the basis of the improvement of method for extracting genomic DNA from tissues. [Method]The genomic DNA with complete primary structure and high purity was obtained from the sheep blood clot after the steps of cutting the sheep blood clot with ophthalmic scissors, cell lysis with tissue DNA extracts and digested by proteinase K, extracting with phenol/chloroform and precipitating with ethanol were performed. [ Result] The concentration of the extracted DNA was 159.90 ±0.70 ng/μl and the ratio of the A260/A280 was 1.80 ＋0.01. The sheep microsatellite locus of BM203 was amplified by using the extracted DNA from the sheep blood clot as template of PCR, and the PCR result was perfect. [Conclusion]This method is simple and feasible, the quantity and quality of the extracted DNA can satisfy the demands for the subsequent researches. It is worth to extending and using for reference.
文摘作为Sigma-Aldrich Co Ltd一部分的Fluka Chemicals研制出一种新的RNA分离盒,其Micro Selelct区具有快速、一步提取RNA的功能。这种盒使用guanidiam硫氰酸盐-酚-氯仿提取法,能获得纯RNA,不含DNA且污染蛋白含量低于用guanidium-CsC1法制备的样品。因为没有离心步骤,所以能同时加工大量样品。Fluka介绍说,这种盒可应用于小规模和大规模制备RNA。
