OBJECTIVE:To compare the effects of carbamylβ-methylcholine chloride(CMCC) administered by intra-acupuncture point injection(IAI),intramuscular injection(IMI),and intravenous injection(IVI),and to analyze the mechani...OBJECTIVE:To compare the effects of carbamylβ-methylcholine chloride(CMCC) administered by intra-acupuncture point injection(IAI),intramuscular injection(IMI),and intravenous injection(IVI),and to analyze the mechanisms.METHODS:In the IAI group,CMCC was injected into the Zusanli acupoint(ST 36) immediately after 30-min stimulation by electro-acupuncture(EA) at the acupoints,and into the femoral vein and skeletal muscle in IVI and IMI groups,respectively.Intra-gastric pressure was detected.The plasma concentration of CMCC was measured at various times.RESULTS:The gastric effect of CMCC in the IVI group was enhanced and attenuated more rapidly than in the other groups.In the IAI group,this effect was significantly stronger than that in the IMI group at 2 min and 15 min,but not significantly different between the two groups at 5 min and 30 min.Plasma concentration of CMCC in the IAI group was similar to that in the IVI group at 2 min,but higher than that in the IMI group.The concentration in the IAI group was higher than that in the IV group and similar to that in the IMI group at 5,15 and 30 min,indicating rapid increase and slower reduction of the plasma concentration of the drug in the IAI group.There was a positive correlation between the plasma concentration of CMCC and intragastric pressure in all groups.CONCLUSION:The effect of IAI with CMCC was stronger than that of IMI and longer-lasting than that of IVI,which correlated with the blood concentration of CMCC.展开更多
Objective： To observe the intervening effect of electroacupuncmre at Ganshu （BL 18） and Zusanli （ST 36） on ERK phosphorylation in rats of hepatofibrosis, and to explore the possible molecular mechanisms of electr...Objective： To observe the intervening effect of electroacupuncmre at Ganshu （BL 18） and Zusanli （ST 36） on ERK phosphorylation in rats of hepatofibrosis, and to explore the possible molecular mechanisms of electroacupunctre for hepatofibrosis. Methods： Forty male Wistar rats were randomly divided into normal group, model group, electroacupuncture group, and medicine group. Rats in the latter three groups were hypodermically injected with the mixture of olive oil and 30% carbon tetrachloride and drank tap water containing 10% alcohol to induce hepatic fibrosis model. Rats in the electroacupuncture group were stimulated at Zusanli （ST 36） and Ganshu （BL 18） by electroacupuncture, and rats in the medicine group were treated with Colchicine. The collagen hyperplasia of rats＇ hepatic tissue was observed by Sirius Red staining, and protein expression of ERK and phosphorylated ERK （P-ERK） were observed by Western Blot. Results： Compared with those in the normal group, collagen deposition in hepatic tissue obviously increased in the model group, and P-ERK protein expression was also up-regulated obviously （P〈0.01）. Compared with those in the model group, collagen deposition in hepatic tissue decreased in the electroacupuncmre and medicine groups, and P-ERK protein expressed was down-regulated （P〈0.01）. The difference between the electroacupuncture and medicine groups had no significance. Conclusion： Electroacupuncture can prevent or delay the development of hepatic fibrosis in rats by down-regulating the protein expression of P-ERK to block MAPK/ERK signal pathway and to suppress the synthesis of extracellular matrix.展开更多
基金Supported by Beijing Natural Science Foundation Grant(grant number 7072053)the National Basic Research Pro-gram of China (973 Program) of China Grant(2006CB504506)+1 种基金the National Natural Science Foundationof China (NSFC) Grants (30873295 and 30801485)the Business fees of China academy of Chinese Medical Sciences for independent Topics of Basic Researchers (ZZ2007001)
文摘OBJECTIVE:To compare the effects of carbamylβ-methylcholine chloride(CMCC) administered by intra-acupuncture point injection(IAI),intramuscular injection(IMI),and intravenous injection(IVI),and to analyze the mechanisms.METHODS:In the IAI group,CMCC was injected into the Zusanli acupoint(ST 36) immediately after 30-min stimulation by electro-acupuncture(EA) at the acupoints,and into the femoral vein and skeletal muscle in IVI and IMI groups,respectively.Intra-gastric pressure was detected.The plasma concentration of CMCC was measured at various times.RESULTS:The gastric effect of CMCC in the IVI group was enhanced and attenuated more rapidly than in the other groups.In the IAI group,this effect was significantly stronger than that in the IMI group at 2 min and 15 min,but not significantly different between the two groups at 5 min and 30 min.Plasma concentration of CMCC in the IAI group was similar to that in the IVI group at 2 min,but higher than that in the IMI group.The concentration in the IAI group was higher than that in the IV group and similar to that in the IMI group at 5,15 and 30 min,indicating rapid increase and slower reduction of the plasma concentration of the drug in the IAI group.There was a positive correlation between the plasma concentration of CMCC and intragastric pressure in all groups.CONCLUSION:The effect of IAI with CMCC was stronger than that of IMI and longer-lasting than that of IVI,which correlated with the blood concentration of CMCC.
基金National Nature Science Foundation (30600833)Shanghai Leading Academic Discipline Project (S30304)
文摘Objective： To observe the intervening effect of electroacupuncmre at Ganshu （BL 18） and Zusanli （ST 36） on ERK phosphorylation in rats of hepatofibrosis, and to explore the possible molecular mechanisms of electroacupunctre for hepatofibrosis. Methods： Forty male Wistar rats were randomly divided into normal group, model group, electroacupuncture group, and medicine group. Rats in the latter three groups were hypodermically injected with the mixture of olive oil and 30% carbon tetrachloride and drank tap water containing 10% alcohol to induce hepatic fibrosis model. Rats in the electroacupuncture group were stimulated at Zusanli （ST 36） and Ganshu （BL 18） by electroacupuncture, and rats in the medicine group were treated with Colchicine. The collagen hyperplasia of rats＇ hepatic tissue was observed by Sirius Red staining, and protein expression of ERK and phosphorylated ERK （P-ERK） were observed by Western Blot. Results： Compared with those in the normal group, collagen deposition in hepatic tissue obviously increased in the model group, and P-ERK protein expression was also up-regulated obviously （P〈0.01）. Compared with those in the model group, collagen deposition in hepatic tissue decreased in the electroacupuncmre and medicine groups, and P-ERK protein expressed was down-regulated （P〈0.01）. The difference between the electroacupuncture and medicine groups had no significance. Conclusion： Electroacupuncture can prevent or delay the development of hepatic fibrosis in rats by down-regulating the protein expression of P-ERK to block MAPK/ERK signal pathway and to suppress the synthesis of extracellular matrix.
