Objective:To investigate the mechanisms of electroacupuncture(EA)at Zusanli(ST 36),Liangmen(ST 21)and Sanyinjiao(SP 6)in intervening diabetic gastroparesis(DGP)based on calcium-activated chloride channel.Methods:Forty...Objective:To investigate the mechanisms of electroacupuncture(EA)at Zusanli(ST 36),Liangmen(ST 21)and Sanyinjiao(SP 6)in intervening diabetic gastroparesis(DGP)based on calcium-activated chloride channel.Methods:Forty Sprague-Dawley rats were randomly divided into four groups,including a normal control group(group A),a model group(group B),an EA group(group C)and a metoclopramide group(group D),with 10 rats in each group.A single intraperitoneal injection of 2%streptozotocin(STZ)combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model.After intervention,gastrointestinal propulsive rate was observed;the expression level of transmembrane protein 16A(TMEM16A)was examined by immunohistochemistry;the Ca2+concentration in interstitial cells of Cajal(ICCs)was detected by immunofluorescence;and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel(ICaCC)in ICCs in gastric antrum.Results:After modeling,the blood glucose levels in group B,group C and group D were significantly increased compared with group A(all P<0.01);after intervention,compared with group B,the blood glucose levels in group C and group D were significantly decreased(P<0.05,P<0.01);the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C(P<0.01).The gastrointestinal propulsive rates in group B,group C and group D were significantly different from that in group A(P<0.01 or P<0.05);the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B(P<0.01,P<0.01).The expressions of TMEM16A in group B and group C were decreased compared with group A(P<0.01,P<0.05);the expressions of TMEM16A in group C and group D were increased compared with group B(P<0.01,P<0.05).The fluorescence intensity of Ca2+was significantly lower in group B than in group A(P<0.01);the fluorescence intensity of Ca2+was significantly higher in group C and group D than in group B(P<0.01,P<0.05).ICaCC in ICCs in group B was significantly decreased compared with group A;ICaCC in group C and group D were increased compared with group B.Conclusion:EA at Zusanli(ST 36),Liangmen(ST 21)and Sanyinjiao(SP 6)can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.展开更多
文摘Objective:To investigate the mechanisms of electroacupuncture(EA)at Zusanli(ST 36),Liangmen(ST 21)and Sanyinjiao(SP 6)in intervening diabetic gastroparesis(DGP)based on calcium-activated chloride channel.Methods:Forty Sprague-Dawley rats were randomly divided into four groups,including a normal control group(group A),a model group(group B),an EA group(group C)and a metoclopramide group(group D),with 10 rats in each group.A single intraperitoneal injection of 2%streptozotocin(STZ)combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model.After intervention,gastrointestinal propulsive rate was observed;the expression level of transmembrane protein 16A(TMEM16A)was examined by immunohistochemistry;the Ca2+concentration in interstitial cells of Cajal(ICCs)was detected by immunofluorescence;and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel(ICaCC)in ICCs in gastric antrum.Results:After modeling,the blood glucose levels in group B,group C and group D were significantly increased compared with group A(all P<0.01);after intervention,compared with group B,the blood glucose levels in group C and group D were significantly decreased(P<0.05,P<0.01);the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C(P<0.01).The gastrointestinal propulsive rates in group B,group C and group D were significantly different from that in group A(P<0.01 or P<0.05);the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B(P<0.01,P<0.01).The expressions of TMEM16A in group B and group C were decreased compared with group A(P<0.01,P<0.05);the expressions of TMEM16A in group C and group D were increased compared with group B(P<0.01,P<0.05).The fluorescence intensity of Ca2+was significantly lower in group B than in group A(P<0.01);the fluorescence intensity of Ca2+was significantly higher in group C and group D than in group B(P<0.01,P<0.05).ICaCC in ICCs in group B was significantly decreased compared with group A;ICaCC in group C and group D were increased compared with group B.Conclusion:EA at Zusanli(ST 36),Liangmen(ST 21)and Sanyinjiao(SP 6)can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.
